CRIS Order Instructions


A molecular pathology CRIS order is required prior to submission of a specimen for molecular pathology testing. The CRIS order is listed under the heading of Anatomic Pathology, sub-heading, Molecular Pathology. The molecular pathology order is driven by specimen sources which are divided into 5 types.

When entering CRIS order for a requested physician, please enter REQUESTED BY physician’s name. This will assure that the requested physician’s name appears correctly on clinical report.


1. Blood Specimen

  1. In CRIS:  Order Entry / Anatomic Pathology / Molecular Pathology, Blood.
  2. Enter a collection date which accurately reflects the date of specimen collection.
  3. Enter in Brief Clinical History to communicate with molecular pathologists.
  4. From “Test Requested”, select Hematopathology and check one or more tests. Or from “Test Requested”, select Exome Germline Variant Control.
  5. Or from “Test Requested”, select COMPASS and check TruSight Oncology 500 (TSO500)


      Available Tests – Hematopathology:

  • B Cell Clonality
  • T Cell Clonality
  • EBV (EBNA2)
  • HHV8
  • HTLV-1
  • BRAF V600E Mutation
  • MYD88 L256P Mutation

      Available Tests – COMPASS:

  • Exome Germline Variant Control (for Whole Exome DNA and RNA Sequencing)
  • TruSight Oncology 500 (TSO500)

Also see liquid specimen collection and handling instructions:

https://ccrod.cancer.gov/confluence/display/CCRLP/Liquid+Specimen+Collection+and+Handling


2. Bone Marrow Aspirate Specimen

  1. In CRIS:  Order Entry / Anatomic Pathology / Molecular Pathology, Bone Marrow.
  2. Enter a collection date which accurately reflects the date of specimen collection.
  3. Enter in Brief Clinical History to communicate with molecular pathologists.
  4. From “Test Requested”, select Hematopathology
  5. from “Test Requested”, select COMPASS and check TruSight Oncology (TSO500) 

      Available Tests – Hematopathology:

  • B Cell Clonality
  • T Cell Clonality
  • EBV (EBNA2)
  • HHV8
  • HTLV-1
  • BRAF V600E Mutation
  • MYD88 L256P Mutation

       Available Tests – COMPASS:

  • TruSight Oncology 500 (TSO500)

Also see liquid specimen collection and handling instructions:

https://ccrod.cancer.gov/confluence/display/CCRLP/Liquid+Specimen+Collection+and+Handling


3. CSF or Pleural Fluid Specimen

  1. In CRIS:  Order Entry / Anatomic Pathology / Molecular Pathology, Body Fluid.
  2. Enter a collection date which accurately reflects the date of specimen collection.
  3. Enter in Brief Clinical History to communicate with molecular pathologists.
  4. From “Specimen Source”, select type of body fluid.
  5. From “Test Requested”, select Hematopathology and check one or more tests.

      Available Tests – Hematopathology:

  • B Cell Clonality
  • T Cell Clonality
  • EBV (EBNA2)
  • HHV8
  • HTLV-1
  • BRAF V600E Mutation
  • MYD88 L256P Mutation

Also see liquid specimen collection and handling instructions:

https://ccrod.cancer.gov/confluence/display/CCRLP/Liquid+Specimen+Collection+and+Handling


4. Tissue Specimen

  1. In CRIS:  Order Entry / Anatomic Pathology / Molecular Pathology, Tissue.
  2. Enter a collection date which accurately reflects the date of specimen collection.
  3. Enter in Brief Clinical History to communicate with molecular pathologists.
  4. From “Test Requested”, select one of 3 test categories (Hematopathology, Single Mutation, COMPASS) to see and select one or more tests.
  5. If COMPASS Whole Exome DNA and RNA Sequencing is selected, you must obtain a written consent from the patient AND put in a separate CRIS order of Exome Germline Variant Control with two options of control materials #1: Molecular Pathology, Blood or #2: Molecular Pathology, Outside Material Other (Type-In) Saliva. Please check consenting and ordering SOP# ADGC-5 at https://ccrod.cancer.gov/confluence/pages/viewpage.action?pageId=73203825#CCRPolicies/StandardOperatingProcedures(SOPs)-ADCR.

      Available Tests – Hematopathology:

  • B Cell Clonality
  • T Cell Clonality
  • EBV (EBNA2)
  • HHV8
  • HTLV-1
  • BRAF V600E Mutation
  • MYD88 L256P Mutation

      Available Tests – Single Mutation:

  • BRAF V600E Mutation
  • MYD88 L256P Mutation

      Available Tests – COMPASS:

  • TruSight Oncology 500 (TSO500)
  • Whole Exome DNA and RNA Sequencing

Also see FFPE tumor tissue requirement and submission instructions:

https://ccrod.cancer.gov/confluence/display/CCRLP/FFPE+Tissue+Submissions


5. Outside Material (only for blood or bone marrow received in your clinic from outside of the NIH Clinical Center or saliva from patient)

  1. In CRIS: Order Entry / Anatomic Pathology / Molecular Pathology, Outside Material.
  2. Enter a collection date which accurately reflects the date of specimen collection.
  3. Enter in Brief Clinical History to communicate with the molecular pathologists.
  4. From “Specimen Source”, select Blood, or Bone Marrow, or Other (Type-In).
  5. From “Test Requested”, select one test category from dropdown list.
  6. Check the test(s) to order.
  7. Label the specimen tube with patient name and NIH MRN. Bring the specimen to the room 3S247.

      Available Tests – Hematopathology:

  • B Cell Clonality
  • T Cell Clonality
  • EBV (EBNA2)
  • HHV8
  • HTLV-1
  • BRAF V600E Mutation
  • MYD88 L256P Mutation

      Available Tests – COMPASS:

  • Exome Germline Variant Control (for Whole Exome DNA and RNA Sequencing)

Also see liquid specimen collection and handling instructions:

https://ccrod.cancer.gov/confluence/display/CCRLP/Liquid+Specimen+Collection+and+Handling
Last updated by Lumelski, Victoria (NIH/NCI) [E] on Dec 08, 2023