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High-Throughput Imaging Facility (HiTIF)

Mission:  To support CCR researchers in the beginning-to-end design and implementation of High-Content imaging (HCI) assays

HCI allows the systematic characterization of multiple complex phenotypes at the single-cell level. HCI can be used to screen either siRNA libraries to probe and characterize molecular pathways of interest, or collections of small molecules to identify compounds with interesting biological properties and therapeutic potential. HCI is also extremely useful for the systematic quantification of exceedingly rare phenotypic events in large heterogeneous populations of cells. The HiTIF is equipped with automated liquid handling instrumentation for library management and dispensing of perturbing agents, with confocal fluorescence microscopy platforms for live, high-throughput image acquisition, and with automated image analysis and secondary data analysis software. We provide HiTIF users with expertise in the use of HCI instrumentation and software and in the design, troubleshooting and implementation of HCI assays to answer a wide range of biological questions.