Miscellaneous Protocols
293TT Cells | A useful cell line with stable expression of SV40 small t and Large T antigens |
Siliconized Plasticware | Sources for siliconized tips and tubes, which may prevent titer loss due to plastic adsorbtion |
Colorimetric SEAP | Alternative method for detecting secreted alkaline phosphatase in the neutralization assay |
Modified Hirt Extract | Spin column-based method for extracting plasmid DNA from mammalian cells. |
DNA Load Dye | Recipe for DNA sample dye for agarose gel analysis. |
mAb production | Method for generating monoclonal antibodies against recombinant antigens. |
Methods for immunofluorescent staining of cultured cells | |
FFPE Staining | Methods for immunofluorescent staining of sections of formalin-fixed paraffin embedded specimens |
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