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Miscellaneous Protocols

293TT CellsA useful cell line with stable expression of SV40 small t and Large T antigens
Siliconized PlasticwareSources for siliconized tips and tubes, which may prevent titer loss due to plastic adsorbtion
Colorimetric SEAPAlternative method for detecting secreted alkaline phosphatase in the neutralization assay
Modified Hirt ExtractSpin column-based method for extracting plasmid DNA from mammalian cells.
DNA Load DyeRecipe for DNA sample dye for agarose gel analysis.
mAb productionMethod for generating monoclonal antibodies against recombinant antigens.
Methods for immunofluorescent staining of cultured cells
FFPE StainingMethods for immunofluorescent staining of sections of formalin-fixed paraffin embedded specimens
Last updated by Buck, Christopher (NIH/NCI) [E] on Oct 21, 2018