LOCUS PYNL2.TXT 6605 BP DS-DNA CIRCULAR SYN 26-MAR-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Plasmid from Blueheron biotechnology. Used for cloning codon optimized genes into it. FEATURES Location/Qualifiers promoter 113..310 /note="EF-1alpha promoter core" promoter 113..1291 /note="EF1a promoter and UTR" exon 311..343 /note="EF-1a exon 1" intron 344..1283 /note="EF-1a intron A" exon 1283..1291 /note="EF-1a exon 2 leader" CDS 1385..>1903 /note="EYFP N-terminal fragment" mutation 1577..1577 /note="T->C makes F64L" CDS 1904..>1927 /note="linker" CDS 1928..>3349 /note="HPV16 L2" mRNA 3422..>4010 /note="WPRE" polyA_signal 4058..>4283 /note="SV40 late polyA" rep_origin 4284..5038 /note="MB1 ori" promoter 5213..5387 /note="SV40 early promoter" repeat_region 5213..5276 /note="SV40 21 bp repeats" rep_origin 5236..5387 /note="SV40 ori" mRNA 5285..5334 /note="SV40 transcription start points" promoter 5422..5477 /note="EM7" CDS 5497..6291 /note="Kan/Neo resistance" polyA_signal 6339..6605 /note="HSV TK pA signal" polyA_site 6397..6415 /note="HSV TK pA site" BASE COUNT 1391 A 2014 C 1816 G 1384 T 0 OTHER ORIGIN - 1 CCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTGCAAAGA TGGATAAAGT TTTAAACAGA 61 GAGGAATCTT TGCAGCTAAT GGACCTTGTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 121 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 181 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 241 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 301 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 361 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 421 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 481 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 541 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 601 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 661 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 721 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 781 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 841 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 901 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 961 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1021 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1081 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1141 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1201 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1261 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA 1321 GATCCATCCT GTACGGAAGT GTTACTTCTG CTCTAAAAGC TGCGGAATTG TACCCGCGGC 1381 CGCCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT 1441 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC 1501 CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC 1561 CACCCTCGTG ACCACCCTCG GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT 1621 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT 1681 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC 1741 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG 1801 GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA 1861 GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGGCTCCG GAGGCACTAG 1921 AGCCACCATG AGGCACAAGA GGAGCGCCAA GAGGACCAAG AGGGCCAGCG CCACCCAGCT 1981 GTACAAGACC TGCAAGCAGG CCGGCACCTG CCCCCCCGAC ATCATCCCCA AGGTGGAGGG 2041 CAAGACCATC GCCGACCAGA TCCTGCAGTA CGGCAGCATG GGCGTGTTCT TCGGCGGCCT 2101 GGGCATCGGC ACCGGCAGCG GCACCGGCGG CAGGACCGGC TACATCCCCC TGGGCACCAG 2161 GCCCCCCACC GCCACCGACA CCCTGGCCCC CGTGAGGCCC CCCCTGACCG TGGACCCCGT 2221 GGGCCCCAGC GACCCCAGCA TCGTGAGCCT GGTGGAGGAG ACCAGCTTCA TCGACGCCGG 2281 CGCCCCCACC AGCGTGCCCA GCATCCCCCC CGACGTGAGC GGCTTCAGCA TCACCACCAG 2341 CACCGACACC ACCCCCGCCA TCCTGGACAT CAACAACACC GTGACCACCG TGACCACCCA 2401 CAACAACCCC ACCTTCACCG ACCCCAGCGT GCTGCAGCCC CCCACCCCCG CCGAGACCGG 2461 CGGCCACTTC ACCCTGAGCA GCAGCACCAT CAGCACCCAC AACTACGAGG AGATCCCCAT 2521 GGACACCTTC ATCGTGAGCA CCAACCCCAA CACCGTGACC AGCAGCACCC CCATCCCCGG 2581 CAGCAGGCCC GTGGCCAGGC TGGGCCTGTA CAGCAGGACC ACCCAGCAGG TGAAGGTGGT 2641 GGACCCCGCC TTCGTGACCA CCCCCACCAA GCTGATCACC TACGACAACC CCGCCTACGA 2701 GGGCATCGAC GTGGACAACA CCCTGTACTT CAGCAGCAAC GACAACAGCA TCAACATCGC 2761 CCCCGACCCC GACTTCCTGG ACATCGTGGC CCTGCACAGG CCCGCCCTGA CCAGCAGGAG 2821 GACCGGCATC AGGTACAGCA GGATCGGCAA CAAGCAGACC CTGAGGACCA GGAGCGGCAA 2881 GAGCATCGGC GCCAAGGTGC ACTACTACTA CGACCTGAGC ACCATCGACC CCGCCGAGGA 2941 GATCGAGCTG CAGACCATCA CCCCCAGCAC CTACACCACC ACCAGCCACG CCGCCAGCCC 3001 CACCAGCATC AACAACGGCC TGTACGACAT CTACGCCGAC GACTTCATCA CCGACACCAG 3061 CACCACCCCC GTGCCCAGCG TGCCCAGCAC CAGCCTGAGC GGCTACATCC CCGCCAACAC 3121 CACCATCCCC TTCGGTGGCG CCTACAACAT CCCCCTGGTG AGCGGCCCCG ACATCCCCAT 3181 CAACATCACC GACCAGGCCC CCAGCCTGAT CCCCATCGTG CCCGGCAGCC CCCAGTACAC 3241 CATCATCGCC GACGCCGGCG ACTTCTACCT GCACCCCAGC TACTACATGC TGAGGAAGAG 3301 GAGGAAGAGG CTGCCCTACT TCTTCAGCGA CGTGAGCCTG GCCGCCTGAA AGCTTTTTGA 3361 ATTCTTTGGA TCCACTAGTG GATCCCCCGG GCTGCAGGAA TTCGATATCA AGCTTATCGA 3421 TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC 3481 TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG 3541 TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT 3601 GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC 3661 TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC 3721 TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT 3781 GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT 3841 CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT 3901 CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT 3961 TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG 4021 TCGGCCGCTT CCCTTTAGTG AGGGTTAATG CTTCGAGCAG ACATGATAAG ATACATTGAT 4081 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT 4141 GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT 4201 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA 4261 AACCTCTACA AATGTGGTAT GGAATTCTTA ATTAACTAGC CATGACCAAA ATCCCTTAAC 4321 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 4381 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 4441 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 4501 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 4561 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 4621 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 4681 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 4741 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 4801 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 4861 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 4921 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 4981 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTA ATTAAGCTGT 5041 ACACTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 5101 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 5161 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 5221 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGAC 5281 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG 5341 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 5401 ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA ATCATCGGCA TAGTATATCG 5461 GCATAGTATA ATACGACTCA CTATAGGAGG GCCACCATGA TTGAACAAGA TGGATTGCAC 5521 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 5581 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 5641 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG ACGAGGCAGC GCGGCTATCG 5701 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 5761 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 5821 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 5881 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 5941 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 6001 GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG AGGATCTCGT CGTGACACAT 6061 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 6121 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 6181 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 6241 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 6301 TGGGGTTCGA AATGACCGAC CAAGCGAATT CGCTAGAGGG AGGCTAACTG AAACACGGAA 6361 GGAGACAATA CCGGAAGGAA CCCGCGCTAT GACGGCAATA AAAAGACAGA ATAAAACGCA 6421 CGGTGTTGGG TCGTTTGTTC ATAAACGCGG GGTTCGGTCC CAGGGCTGGC ACTCTGTCGA 6481 TACCCCACCG AGACCCCATT GGGGCCAATA CGCCCGCGTT TCTTCCTTTT CCCCACCCCA 6541 CCCCCCAAGT TCGGGTGAAG GCCCAGGGCT CGCAGCCAAC GTCGGGGCGG CAGGCCCTGC 6601 CATAG //