LOCUS PYCL2.TXT 6287 BP DS-DNA CIRCULAR SYN 26-MAR-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Plasmid from Blueheron biotechnology. Used for cloning codon optimized genes into it. FEATURES Location/Qualifiers promoter 113..310 /note="EF-1alpha promoter core" promoter 113..1291 /note="EF1a promoter and UTR" exon 311..343 /note="EF-1a exon 1" intron 344..1283 /note="EF-1a intron A" exon 1283..1291 /note="EF-1a exon 2 leader" CDS 1385..1387 /note="artificial start" CDS <1388..>1585 /note="EYFP C-terminal fragment" CDS 1586..>1609 /note="linker" CDS 1610..>3031 /note="HPV16 L2" mRNA 3104..>3692 /note="WPRE" polyA_signal 3740..>3965 /note="SV40 late polyA" rep_origin 3966..4720 /note="MB1 ori" promoter 4895..5069 /note="SV40 early promoter" repeat_region 4895..4958 /note="SV40 21 bp repeats" rep_origin 4918..5069 /note="SV40 ori" mRNA 4967..5016 /note="SV40 transcription start points" promoter 5104..5159 /note="EM7" CDS 5179..5973 /note="Kan/Neo resistance" polyA_signal 6021..6287 /note="HSV TK pA signal" polyA_site 6079..6097 /note="HSV TK pA site" BASE COUNT 1310 A 1921 C 1722 G 1334 T 0 OTHER ORIGIN - 1 CCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTGCAAAGA TGGATAAAGT TTTAAACAGA 61 GAGGAATCTT TGCAGCTAAT GGACCTTGTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 121 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 181 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 241 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 301 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 361 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 421 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 481 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 541 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 601 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 661 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 721 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 781 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 841 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 901 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 961 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1021 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1081 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1141 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1201 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1261 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA 1321 GATCCATCCT GTACGGAAGT GTTACTTCTG CTCTAAAAGC TGCGGAATTG TACCCGCGGC 1381 CGCCATGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA 1441 CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC CAGTCCGCCC TGAGCAAAGA 1501 CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC 1561 TCTCGGCATG GACGAGCTGT ACAAGGGCTC CGGAGGCACT AGAGCCACCA TGAGGCACAA 1621 GAGGAGCGCC AAGAGGACCA AGAGGGCCAG CGCCACCCAG CTGTACAAGA CCTGCAAGCA 1681 GGCCGGCACC TGCCCCCCCG ACATCATCCC CAAGGTGGAG GGCAAGACCA TCGCCGACCA 1741 GATCCTGCAG TACGGCAGCA TGGGCGTGTT CTTCGGCGGC CTGGGCATCG GCACCGGCAG 1801 CGGCACCGGC GGCAGGACCG GCTACATCCC CCTGGGCACC AGGCCCCCCA CCGCCACCGA 1861 CACCCTGGCC CCCGTGAGGC CCCCCCTGAC CGTGGACCCC GTGGGCCCCA GCGACCCCAG 1921 CATCGTGAGC CTGGTGGAGG AGACCAGCTT CATCGACGCC GGCGCCCCCA CCAGCGTGCC 1981 CAGCATCCCC CCCGACGTGA GCGGCTTCAG CATCACCACC AGCACCGACA CCACCCCCGC 2041 CATCCTGGAC ATCAACAACA CCGTGACCAC CGTGACCACC CACAACAACC CCACCTTCAC 2101 CGACCCCAGC GTGCTGCAGC CCCCCACCCC CGCCGAGACC GGCGGCCACT TCACCCTGAG 2161 CAGCAGCACC ATCAGCACCC ACAACTACGA GGAGATCCCC ATGGACACCT TCATCGTGAG 2221 CACCAACCCC AACACCGTGA CCAGCAGCAC CCCCATCCCC GGCAGCAGGC CCGTGGCCAG 2281 GCTGGGCCTG TACAGCAGGA CCACCCAGCA GGTGAAGGTG GTGGACCCCG CCTTCGTGAC 2341 CACCCCCACC AAGCTGATCA CCTACGACAA CCCCGCCTAC GAGGGCATCG ACGTGGACAA 2401 CACCCTGTAC TTCAGCAGCA ACGACAACAG CATCAACATC GCCCCCGACC CCGACTTCCT 2461 GGACATCGTG GCCCTGCACA GGCCCGCCCT GACCAGCAGG AGGACCGGCA TCAGGTACAG 2521 CAGGATCGGC AACAAGCAGA CCCTGAGGAC CAGGAGCGGC AAGAGCATCG GCGCCAAGGT 2581 GCACTACTAC TACGACCTGA GCACCATCGA CCCCGCCGAG GAGATCGAGC TGCAGACCAT 2641 CACCCCCAGC ACCTACACCA CCACCAGCCA CGCCGCCAGC CCCACCAGCA TCAACAACGG 2701 CCTGTACGAC ATCTACGCCG ACGACTTCAT CACCGACACC AGCACCACCC CCGTGCCCAG 2761 CGTGCCCAGC ACCAGCCTGA GCGGCTACAT CCCCGCCAAC ACCACCATCC CCTTCGGTGG 2821 CGCCTACAAC ATCCCCCTGG TGAGCGGCCC CGACATCCCC ATCAACATCA CCGACCAGGC 2881 CCCCAGCCTG ATCCCCATCG TGCCCGGCAG CCCCCAGTAC ACCATCATCG CCGACGCCGG 2941 CGACTTCTAC CTGCACCCCA GCTACTACAT GCTGAGGAAG AGGAGGAAGA GGCTGCCCTA 3001 CTTCTTCAGC GACGTGAGCC TGGCCGCCTG AAAGCTTTTT GAATTCTTTG GATCCACTAG 3061 TGGATCCCCC GGGCTGCAGG AATTCGATAT CAAGCTTATC GATAATCAAC CTCTGGATTA 3121 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG 3181 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC 3241 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA 3301 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC 3361 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT 3421 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC 3481 CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG TTGCCACCTG 3541 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC 3601 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC 3661 GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGTCGGCCGC TTCCCTTTAG 3721 TGAGGGTTAA TGCTTCGAGC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA 3781 ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT 3841 GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT 3901 CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT 3961 ATGGAATTCT TAATTAACTA GCCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 4021 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 4081 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 4141 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 4201 CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 4261 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 4321 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 4381 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 4441 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 4501 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 4561 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 4621 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 4681 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TAATTAAGCT GTACACTGTG GAATGTGTGT 4741 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 4801 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 4861 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 4921 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG ACTGACTAAT TTTTTTTATT 4981 TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 5041 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 5101 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACT 5161 CACTATAGGA GGGCCACCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 5221 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 5281 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 5341 GGTGCCCTGA ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 5401 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 5461 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 5521 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 5581 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 5641 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 5701 AAGGCGAGCA TGCCCGACGG CGAGGATCTC GTCGTGACAC ATGGCGATGC CTGCTTGCCG 5761 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 5821 GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 5881 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 5941 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 6001 ACCAAGCGAA TTCGCTAGAG GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG 6061 AACCCGCGCT ATGACGGCAA TAAAAAGACA GAATAAAACG CACGGTGTTG GGTCGTTTGT 6121 TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA 6181 TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA 6241 AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAG //