LOCUS PU-7257 (L 7256 BP DS-DNA CIRCULAR SYN 10-MAR-1998 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers CDS complement(<228..1543) /note="LacZ [Split]" frag 228..1645 /note="4814 to 6231 of pSC11 MCS2 (annot)" promoter <1661..>2344 /note="URR fragment [Split]" rep_origin 2205..2282 /note="Minimal Ori (Fields p2211)" misc_RNA 2342..>2348 /note="P89 cap site (Type 1)" polyA_signal 2378..2647 /note="HSV TK pA signal" polyA_site 2436..2454 /note="HSV TK pA sites" promoter 2760..3938 /note="EF1a promoter" enhancer 2760..2957 /note="EF-1alpha promoter core domain" exon 2958..2990 /note="EF-1a exon 1" intron 2991..3930 /note="EF-1a intron A" exon 3930..3938 /note="EF-1a exon 2 leader" CDS 3999..4715 /note="EGFP (weighs 27kD)" polyA_signal 4730..5007 /note="BGH polyA" rep_origin 5437..6110 /note="Coli Ori" CDS complement(6262..7120) /note="AmpR" promoter complement(7155..7190) /note="AmpR's promoter" BASE COUNT 1690 A 1921 C 1954 G 1691 T 0 OTHER ORIGIN - 1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTCGCTCA 241 TCGCCGGTAG CCAGCGCGGA TCATCGGTCA GACGATTCAT TGGCACCATG CCGTGGGTTT 301 CAATATTGGC TTCATCCACC ACATACAGGC CGTAGCGGTC GCACAGCGTG TACCACAGCG 361 GATGGTTCGG ATAATGCGAA CAGCGCACGG CGTTAAAGTT GTTCTGCTTC ATCAGCAGGA 421 TATCCTGCAC CATCGTCTGC TCATCCATGA CCTGACCATG CAGAGGATGA TGCTCGTGAC 481 GGTTAACGCC TCGAATCAGC AACGGCTTGC CGTTCAGCAG CAGCAGACCA TTTTCAATCC 541 GCACCTCGCG GAAACCGACA TCGCAGGCTT CTGCTTCAAT CAGCGTGCCG TCGGCGGTGT 601 GCAGTTCAAC CACCGCACGA TAGAGATTCG GGATTTCGGC GCTCCACAGT TTCGGGTTTT 661 CGACGTTGAG ACGTAGTGTG ACGCGATCGG CATAACCACC ACGCTCATCG ATAATTTCAC 721 CGCCGAAAGG CGCGGTGCCG CTGGCGACCT GCGTTTCACC CTGCCATAAA GAAACTGTTA 781 CCCGTAGGTA GTCACGCAAC TCGCCGCACA TCTGAACTTC AGCCTCCAGT ACAGCGCGGC 841 TGAAATCATC ATTAAAGCGA GTGGCAACAT GGAAATCGCT GATTTGTGTA GTCGGTTTAT 901 GCAGCAACGA GACGTCACGG AAAATGCCGC TCATCCGCCA CATATCCTGA TCTTCCAGAT 961 AACTGCCGTC ACTCCAACGC AGCACCATCA CCGCGAGGCG GTTTTCTCCG GCGCGTAAAA 1021 ATGCGCTCAG GTCAAATTCA GACGGCAAAC GACTGTCCTG GCCGTAACCG ACCCAGCGCC 1081 CGTTGCACCA CAGATGAAAC GCCGAGTTAA CGCCATCAAA AATAATTCGC GTCTGGCCTT 1141 CCTGTAGCCA GCTTTCATCA ACATTAAATG TGAGCGAGTA ACAACCCGTC GGATTCTCCG 1201 TGGGAACAAA CGGCGGATTG ACCGTAATGG GATAGGTTAC GTTGGTGTAG ATGGGCGCAT 1261 CGTAACCGTG CATCTGCCAG TTTGAGGGGA CGACGACAGT ATCGGCCTCA GGAAGATCGC 1321 ACTCCAGCCA GCTTTCCGGC ACCGCTTCTG GTGCCGGAAA CCAGGCAAAG CGCCATTCGC 1381 CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC 1441 AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC 1501 AGTCACGACG TTGTAAAACG ACGGGATCCC TCGAGGAATT CATTTATAGC ATAGAAAAAA 1561 ACAAAATGAA ATTCTACTAT ATTTTTACAT ACATATATTC TAAATATGAA AGTGGTGATT 1621 GTGACTAGCG TAGCATCGCT TCTAGTTAGT TATTACTAGC CGCGTGCAAA GCACCGGCGG 1681 CGGTAGATGC GGGGTAAGTA CTGAATTTTA ATTCGACCTA TCCCGGTAAA GCGAAAGCGA 1741 CACGCTTTTT TTTCACACAT AGCGGGACCG AACACGTTAT AAGTATCGAT TAGGTCTATT 1801 TTTGTCTCTC TGTCGGAACC AGAACTGGTA AAAGTTTCCA TTGCGTCTGG GCTTGTCTAT 1861 CATTGCGTCT CTATGGTTTT TGGAGGATTA GACCGGGCCA CCAGTAATGG TGCATAGCGG 1921 ATGTCTGTAC CGCCATCGGT GCACCGATAT AGGTTTGGGG CTCCCCAAGG GACTGCTGGG 1981 ATGACAGCTT CATATTATAT TGAATGGGCG CATAATCAGC TTAATTGGTG AGGACAAGCT 2041 ACAAGTTGTA ACCTGATCTC CACAAAGTAC CGTTGCCGGT CGGGGTCAAA CCGTCTTCGG 2101 TGCTCGAAAC CGCCTTAAAC TACAGACAGG TCCCAGCCAA GTAGGCGGAT CAAAACCTCA 2161 AAAAGGCGGG AGCCAATCAA AATGCAGCAT TATATTTTAA GCTCACCGAA ACCGGTAAGT 2221 AAAGACTATG TATTTTTTCC CAGTGAATAA TTGTTGTTAA CAATAATCAC ACCATCACCG 2281 TTTTTTCAAG CGGGAAAAAA TAGCCAGCTA ACTATAAAAA GCTGCTGACA GACCCCGGTT 2341 TTCATTAAGC TTGGTACCGA GCTCGGATCC ACTAGGGGGA GGCTAACTGA AACACGGAAG 2401 GAGACAATAC CGGAAGGAAC CCGCGCTATG ACGGCAATAA AAAGACAGAA TAAAACGCAC 2461 GGTGTTGGGT CGTTTGTTCA TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT 2521 ACCCCACCGA GACCCCATTG GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC 2581 CCCCCAAGTT CGGGTGAAGG CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC 2641 ATAGCCTCAG CTACCGGACT CAGATCTCGA GCTCAAGCTT GCAAAGATGG ATAAAGTTTT 2701 AAACAGAGAG GAATCTTTGC AGCTAATGGA CCTTGTAGGT CTTGAAAGGA GTGGGAATTG 2761 GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG 2821 GAGGGGTCGG CAATTGAACC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG 2881 ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG 2941 TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG GTAAGTGCCG 3001 TGTGTGGTTC CCGCGGGCCT GGCCTCTTTA CGGGTTATGG CCCTTGCGTG CCTTGAATTA 3061 CTTCCACCTG GCTGCAGTAC GTGATTCTTG ATCCCGAGCT TCGGGTTGGA AGTGGGTGGG 3121 AGAGTTCGAG GCCTTGCGCT TAAGGAGCCC CTTCGCCTCG TGCTTGAGTT GAGGCCTGGC 3181 CTGGGCGCTG GGGCCGCCGC GTGCGAATCT GGTGGCACCT TCGCGCCTGT CTCGCTGCTT 3241 TCGATAAGTC TCTAGCCATT TAAAATTTTT GATGACCTGC TGCGACGCTT TTTTTCTGGC 3301 AAGATAGTCT TGTAAATGCG GGCCAAGATC TGCACACTGG TATTTCGGTT TTTGGGGCCG 3361 CGGGCGGCGA CGGGGCCCGT GCGTCCCAGC GCACATGTTC GGCGAGGCGG GGCCTGCGAG 3421 CGCGGCCACC GAGAATCGGA CGGGGGTAGT CTCAAGCTGG CCGGCCTGCT CTGGTGCCTG 3481 GCCTCGCGCC GCCGTGTATC GCCCCGCCCT GGGCGGCAAG GCTGGCCCGG TCGGCACCAG 3541 TTGCGTGAGC GGAAAGATGG CCGCTTCCCG GCCCTGCTGC AGGGAGCTCA AAATGGAGGA 3601 CGCGGCGCTC GGGAGAGCGG GCGGGTGAGT CACCCACACA AAGGAAAAGG GCCTTTCCGT 3661 CCTCAGCCGT CGCTTCATGT GACTCCACGG AGTACCGGGC GCCGTCCAGG CACCTCGATT 3721 AGTTCTCGAG CTTTTGGAGT ACGTCGTCTT TAGGTTGGGG GGAGGGGTTT TATGCGATGG 3781 AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC TTGATGTAAT 3841 TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG 3901 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGA ATTCTGTAGA GATCCCTCGA 3961 CCTCGAGATC CATTGTGCTG GATCCACCGG TCGCCACCAT GGTGAGCAAG GGCGAGGAGC 4021 TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT 4081 TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA 4141 TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG 4201 GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG 4261 CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA 4321 AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG 4381 GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA 4441 GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA 4501 TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC 4561 CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC 4621 TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG 4681 CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCCTAGA GCTCGCTGAT 4741 CAGCCTCGAC TGTGCCTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC 4801 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC 4861 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG 4921 GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA 4981 GGCGGAAAGA ACCAGCTGGG GCTCGAGTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 5041 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 5101 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 5161 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 5221 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 5281 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 5341 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 5401 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 5461 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 5521 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 5581 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 5641 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT 5701 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 5761 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 5821 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 5881 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 5941 GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 6001 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 6061 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 6121 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 6181 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 6241 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 6301 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 6361 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 6421 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 6481 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 6541 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 6601 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 6661 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 6721 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 6781 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 6841 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 6901 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 6961 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 7021 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 7081 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 7141 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 7201 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTC //