LOCUS P16L2HA.GB 6080 BP DS-DNA CIRCULAR SYN 26-MAR-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Plasmid from Blueheron biotechnology. Used for cloning codon optimized genes into it. FEATURES Location/Qualifiers promoter 113..310 /note="EF-1alpha promoter core" promoter 113..1291 /note="EF1a promoter and UTR" exon 311..343 /note="EF-1a exon 1" intron 344..1283 /note="EF-1a intron A" exon 1283..1291 /note="EF-1a exon 2 leader" CDS 1396..>2814 /note="HPV16 L2 (codon modified)" CDS <2815..2817 /note="linker gly" CDS 2818..>2847 /note="HA tag" mRNA 2897..>3485 /note="WPRE" polyA_signal 3533..>3758 /note="SV40 late polyA" rep_origin 3759..4513 /note="MB1 ori" promoter 4688..4862 /note="SV40 early promoter" repeat_region 4688..4751 /note="SV40 21 bp repeats" rep_origin 4711..4862 /note="SV40 ori" mRNA 4760..4809 /note="SV40 transcription start points" promoter 4897..4952 /note="EM7" CDS 4972..5766 /note="Kan/Neo resistance" polyA_signal 5814..6080 /note="HSV TK pA signal" polyA_site 5872..5890 /note="HSV TK pA site" BASE COUNT 1264 A 1852 C 1664 G 1300 T 0 OTHER ORIGIN - 1 CCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTGCAAAGA TGGATAAAGT TTTAAACAGA 61 GAGGAATCTT TGCAGCTAAT GGACCTTGTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 121 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 181 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 241 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 301 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 361 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 421 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 481 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 541 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 601 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 661 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 721 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 781 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 841 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 901 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 961 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1021 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1081 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1141 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1201 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1261 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA 1321 GATCCATCCT GTACGGAAGT GTTACTTCTG CTCTAAAAGC TGCGGAATTG TACCCGCGGC 1381 CGCTCTAGAG CCACCATGAG GCACAAGAGG AGCGCCAAGA GGACCAAGAG GGCCAGCGCC 1441 ACCCAGCTGT ACAAGACCTG CAAGCAGGCC GGCACCTGCC CCCCCGACAT CATCCCCAAG 1501 GTGGAGGGCA AGACCATCGC CGACCAGATC CTGCAGTACG GCAGCATGGG CGTGTTCTTC 1561 GGCGGCCTGG GCATCGGCAC CGGCAGCGGC ACCGGCGGCA GGACCGGCTA CATCCCCCTG 1621 GGCACCAGGC CCCCCACCGC CACCGACACC CTGGCCCCCG TGAGGCCCCC CCTGACCGTG 1681 GACCCCGTGG GCCCCAGCGA CCCCAGCATC GTGAGCCTGG TGGAGGAGAC CAGCTTCATC 1741 GACGCCGGCG CCCCCACCAG CGTGCCCAGC ATCCCCCCCG ACGTGAGCGG CTTCAGCATC 1801 ACCACCAGCA CCGACACCAC CCCCGCCATC CTGGACATCA ACAACACCGT GACCACCGTG 1861 ACCACCCACA ACAACCCCAC CTTCACCGAC CCCAGCGTGC TGCAGCCCCC CACCCCCGCC 1921 GAGACCGGCG GCCACTTCAC CCTGAGCAGC AGCACCATCA GCACCCACAA CTACGAGGAG 1981 ATCCCCATGG ACACCTTCAT CGTGAGCACC AACCCCAACA CCGTGACCAG CAGCACCCCC 2041 ATCCCCGGCA GCAGGCCCGT GGCCAGGCTG GGCCTGTACA GCAGGACCAC CCAGCAGGTG 2101 AAGGTGGTGG ACCCCGCCTT CGTGACCACC CCCACCAAGC TGATCACCTA CGACAACCCC 2161 GCCTACGAGG GCATCGACGT GGACAACACC CTGTACTTCA GCAGCAACGA CAACAGCATC 2221 AACATCGCCC CCGACCCCGA CTTCCTGGAC ATCGTGGCCC TGCACAGGCC CGCCCTGACC 2281 AGCAGGAGGA CCGGCATCAG GTACAGCAGG ATCGGCAACA AGCAGACCCT GAGGACCAGG 2341 AGCGGCAAGA GCATCGGCGC CAAGGTGCAC TACTACTACG ACCTGAGCAC CATCGACCCC 2401 GCCGAGGAGA TCGAGCTGCA GACCATCACC CCCAGCACCT ACACCACCAC CAGCCACGCC 2461 GCCAGCCCCA CCAGCATCAA CAACGGCCTG TACGACATCT ACGCCGACGA CTTCATCACC 2521 GACACCAGCA CCACCCCCGT GCCCAGCGTG CCCAGCACCA GCCTGAGCGG CTACATCCCC 2581 GCCAACACCA CCATCCCCTT CGGTGGCGCC TACAACATCC CCCTGGTGAG CGGCCCCGAC 2641 ATCCCCATCA ACATCACCGA CCAGGCCCCC AGCCTGATCC CCATCGTGCC CGGCAGCCCC 2701 CAGTACACCA TCATCGCCGA CGCCGGCGAC TTCTACCTGC ACCCCAGCTA CTACATGCTG 2761 AGGAAGAGGA GGAAGAGGCT GCCCTACTTC TTCAGCGACG TGAGCCTGGC CGCCGGATAC 2821 CCCTACGACG TCCCCGACTA CGCCTGAGAC TAGTGGATCC CCCGGGCTGC AGGAATTCGA 2881 TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT 2941 TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA 3001 TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC 3061 TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC 3121 TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT 3181 CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG 3241 GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC 3301 CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA 3361 CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG 3421 GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC 3481 CCCGCATCGA TACCGTCGGC CGCTTCCCTT TAGTGAGGGT TAATGCTTCG AGCAGACATG 3541 ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT 3601 ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA 3661 GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT 3721 TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGAAT TCTTAATTAA CTAGCCATGA 3781 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 3841 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 3901 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 3961 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG 4021 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 4081 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 4141 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 4201 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC 4261 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 4321 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 4381 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 4441 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 4501 TCTTAATTAA GCTGTACACT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 4561 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 4621 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 4681 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 4741 TCTCCGCCCC ATGACTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC 4801 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 4861 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAGC ACGTGTTGAC AATTAATCAT 4921 CGGCATAGTA TATCGGCATA GTATAATACG ACTCACTATA GGAGGGCCAC CATGATTGAA 4981 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC 5041 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 5101 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG 5161 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT 5221 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 5281 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG 5341 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 5401 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG 5461 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA CGGCGAGGAT 5521 CTCGTCGTGA CACATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 5581 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG 5641 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 5701 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC 5761 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GAATTCGCTA GAGGGAGGCT 5821 AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG 5881 ACAGAATAAA ACGCACGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG 5941 CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC 6001 CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG 6061 GGCGGCAGGC CCTGCCATAG //