We demonstrated that the sites of interaction of HMGN with nucleosomes partially overlap the binding sites of histone H1 in chromatin (Alfonso PG, et al. J Mol Biol. 236: 189-98, 1994).
In collaboration with the Hansen Laboratory, we demonstrated that HMGN1 counteracts the repressive activity of histone H1 (Ding HF, et al. Mol Cell Biol. 17: 5843-55 1997).
We demonstrated that HMGN5 competes with H1 and alters transcription and chromatin structure (Rochman M, et al. Mol Cell. 35: 642-56, 2009).
We are interested in the mechanism whereby possible H1-HMGN interactions affect the structure and function of chromatin.