| Pseudovirus Production | Production of pseudoviruses (or capsids) using 293TT cells, including purification by ultracentrifugation through an iodixanol (Optiprep) gradient |
| Revised Production | An updated pseudovirus harvest method that removes "cold" capsids containing cellular DNA fragments |
| Improved Maturation | A protocol modification for achieving more uniform capsid maturation |
| BKV Harvest | Protocol modifications designed to facilitate harvest of BK polyomavirus-based reporter vectors and virions |
| Native MCV Production | Methods for producing and propagating Merkel cell polyomavirus virions in cell culture |
| Propagation | Use of a self-packaging L1+L2 plasmid for infectious amplification of pseudovirus stocks |
| Gel Filtration | Rapid purification of pseudovirus out of crude cell lysates (or Optiprep) using agarose gel filtration columns |
| Dye Conjugation | Coupling AlexaFluor dyes to capsids |
| Encapsidated DNA Analysis | Extraction and analysis of packaged DNA |
Silent modification of genes to remove expression-inhibitory elements (MS Excel file download) | |
| Reconstitution of cloned HPV genomes by intramolecular re-ligation | |
| Modified Hirt Extract | Spin column-based method for extracting plasmid DNA from mammalian cells |
| 293TT Cells | An HEK-293T based cell line modified to express high levels of SV40 Large T antigen |