Experimental Pathology Laboratory (EPL)

Dr. Stephen Hewitt, Section Head, EPL
Building 10, Room 6B 
Phone: 301-496-0040 

Dr. Svetlana Pack, Deputy Section Head, EPL
Building 10, Room 2N115
Phone: 301-451-2723 

EPL Core Services:

Histology  

Frozen Tissue

• Cryosections
• H&Es

Fixed Tissue (Human & Animal) 

• Tissue Fixation, including alternative fixatives
• Tissue Impregnation & Embedding
• Cell block preparation (agarose)
• Microtomy
• Histology Stains (H&E, PAS, Masson, additional stains with development and validation)

Immunohistochemistry   

• Non-clinical Abs on human tissue (formal menu in development)
• Animal tissue
• Double staining (with development and validation)
• Fluorescent IHC with 2ndary Abs
• IHC on Cryosections (requires assay development and validation)

In Situ Assays

• DNA (FISH)
• RNA (flourescence or chromagenic via bDNA)

Laser Capture Microdisection

•  Pixel II (multiple), Veritas, and XT instruments from Arcturus/Life Technologies

Tissue Microarray

• 0.6-2.0 mm core based TMAs

Imaging

• Whole Slide Imaging (20X and 40X)
• Whole Slide Image Hosting
• Upright fluorescent microscope imaging
• Inverted microscope with fluorescent imaging capacity