LOCUS pFx7MCS 8608 bp DNA circular SYN 22-May-2024 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE - ORGANISM . . COMMENT Annotated with pLannotate v1.2.2 FEATURES Location/Qualifiers rep_origin complement(2..1346) /note="2 micron origin" rep_origin 3..1349 /note="2# ori" regulatory 183..287 /regulatory_class="terminator" /note="FLP terminator" CDS complement(1503..1664) /note="RAF1 (fragment)" regulatory 1686..1711 /regulatory_class="promoter" /note="LEU2 promoter (fragment)" CDS 1715..1753 /note="LEU2 (fragment)" 3'UTR complement(1754..1887) /note="FDH1 3' UTR" CDS complement(join(1888..3009,3245..3268)) /note="formaldehyde dehydrogenase from Candida maltosa; confers formaldehyde resistance; expressed from a spliced mRNA" /product="FDH1" regulatory complement(3292..3702) /regulatory_class="promoter" /note="FDH1 promoter" regulatory complement(3357..3380) /regulatory_class="promoter" /note="ARG4 promoter (fragment)" regulatory complement(3399..3418) /regulatory_class="" /note="CAAT" CDS complement(3633..3773) /note="MacVector changed the stop location from 1956 to 1956 based on the universal genetic code" /note="RAF1 (fragment)" misc_feature 3773..6406 /note="pUC18" CDS 3780..3875 /note="MacVector changed the stop location from 2198 to 2198 based on the universal genetic code" /note="lacZ# (fragment)" primer_bind complement(3781..3798) /note="M13-fwd" CDS 3868..4029 /note="MacVector changed the stop location from 2354 to 2354 based on the universal genetic code" /note="LacZ alpha" misc_feature complement(3939..4038) /note="bom (fragment)" CDS complement(4140..4175) /note="rop (fragment)" regulatory 4270..4374 /regulatory_class="promoter" /note="AmpR promoter" CDS 4375..5235 /note="AmpR" primer_bind complement(4593..4612) /note="Ampicillin resistance gene, reverse primer" /note="Amp-R" rep_origin 5406..5994 /note="ori" rep_origin 5426..6008 /note="pMB1 origin (pBR322)" /note="pMB1 ori" misc_feature 5718 /note="Escherichia coli replication initiation base" CDS 6177..6269 /note="lacI (fragment)" protein_bind 6282..6303 /note="CAP binding site" regulatory 6318..6348 /regulatory_class="promoter" /note="lac promoter" misc_binding 6352..6374 /note="LacO" protein_bind 6356..6372 /note="lac operator" primer_bind 6380..6400 /note="M13-rev" regulatory 6430..6799 /regulatory_class="enhancer" /note="GAL10-UAS" protein_bind complement(6608..6725) /note="UAS" misc_feature 6822..7084 /note="PYK1-Pr" misc_feature 7091..7097 /note="yeast Kozak" CDS 7098..8186 /product="VP1" CDS 8234..8323 /note="PGK1 (fragment)" regulatory 8238..8608 /regulatory_class="terminator" /note="PGK1 terminator" ORIGIN 1 ATGATCCAAT ATCAAAGGAA ATGATAGCAT TGAAGGATGA GACTAATCCA ATTGAGGAGT 61 GGCAGCATAT AGAACAGCTA AAGGGTAGTG CTGAAGGAAG CATACGATAC CCCGCATGGA 121 ATGGGATAAT ATCACAGGAG GTACTAGACT ACCTTTCATC CTACATAAAT AGACGCATAT 181 AAGTACGCAT TTAAGCATAA ACACGCACTA TGCCGTTCTT CTCATGTATA TATATATACA 241 GGCAACACGC AGATATAGGT GCGACGTGAA CAGTGAGCTG TATGTGCGCA GCTCGCGTTG 301 CATTTTCGGA AGCGCTCGTT TTCGGAAACG CTTTGAAGTT CCTATTCCGA AGTTCCTATT 361 CTCTAGCTAG AAAGTATAGG AACTTCAGAG CGCTTTTGAA AACCAAAAGC GCTCTGAAGA 421 CGCACTTTCA AAAAACCAAA AACGCACCGG ACTGTAACGA GCTACTAAAA TATTGCGAAT 481 ACCGCTTCCA CAAACATTGC TCAAAAGTAT CTCTTTGCTA TATATCTCTG TGCTATATCC 541 CTATATAACC TACCCATCCA CCTTTCGCTC CTTGAACTTG CATCTAAACT CGACCTCTAC 601 ATTTTTTATG TTTATCTCTA GTATTACTCT TTAGACAAAA AAATTGTAGT AAGAACTATT 661 CATAGAGTGA ATCGAAAACA ATACGAAAAT GTAAACATTT CCTATACGTA GTATATAGAG 721 ACAAAATAGA AGAAACCGTT CATAATTTTC TGACCAATGA AGAATCATCA ACGCTATCAC 781 TTTCTGTTCA CAAAGTATGC GCAATCCACA TCGGTATAGA ATATAATCGG GGATGCCTTT 841 ATCTTGAAAA AATGCACCCG CAGCTTCGCT AGTAATCAGT AAACGCGGGA AGTGGAGTCA 901 GGCTTTTTTT ATGGAAGAGA AAATAGACAC CAAAGTAGCC TTCTTCTAAC CTTAACGGAC 961 CTACAGTGCA AAAAGTTATC AAGAGACTGC ATTATAGAGC GCACAAAGGA GAAAAAAAGT 1021 AATCTAAGAT GCTTTGTTAG AAAAATAGCG CTCTCGGGAT GCATTTTTGT AGAACAAAAA 1081 AGAAGTATAG ATTCTTTGTT GGTAAAATAG CGCTCTCGCG TTGCATTTCT GTTCTGTAAA 1141 AATGCAGCTC AGATTCTTTG TTTGAAAAAT TAGCGCTCTC GCGTTGCATT TTTGTTTTAC 1201 AAAAATGAAG CACAGATTCT TCGTTGGTAA AATAGCGCTT TCGCGTTGCA TTTCTGTTCT 1261 GTAAAAATGC AGCTCAGATT CTTTGTTTGA AAAATTAGCG CTCTCGCGTT GCATTTTTGT 1321 TCTACAAAAT GAAGCACAGA TGCTTCGTTA ACAAAGATAT GCTATTGAAG TGCAAGATGG 1381 AAACGCAGAA AATGAACCGG GGATGCGACG TGCAAGATTA CCTATGCAAT AGATGCAATA 1441 GTTTCTCCAG GAACCGAAAT ACATACATTG TCTTCCGTAA AGCGCTAGAC TATATATTAT 1501 TATACAGGTT CAAATATACT ATCTGTTTCA GGGAAAACTC CCAGGTTCGG ATGTTCAAAA 1561 TTCAATGATG GGTAACAAGT ACGATCGTAA ATCTGTAAAA CAGTTTGTCG GATATTAGGC 1621 TGTATCTCCT CAAAGCGTAT TCGAATATCA TTGAGAAGCT GCATTTTTTT TTTTTTTTTT 1681 TTTTTTATAT ATATTTCAAG GATATACCAT TGTAATGTCT GCCCCTAAGA AGATCGTCGT 1741 TTTGCCAGGT GACGATTCAG ATAATGAATA GAATTTTTAG TAATCCATTT TTTATTTTTA 1801 TTTTTATTTG AAGGTTATAA TAATAGATAA TATATACGTA TCTTAATTAC AAACGAACAA 1861 ACAAACAACT TAAAGTTTGC TGTCTAGTCA TGACAAGTCA ACAACAGCTC TGATACAATC 1921 ACCAGCATGC ATTTCTTCAA AAGCTTTGTT AATGGCTGCC AAAGGTTCTC TGTGGGTAAT 1981 GAATTCTTCA ACCTTCAATT TACCATCCAA GTAATTGTTA ACAATTCCTG GTAATTGGGA 2041 TCTACCTTTA ACACCACCGA AAGCAGCTCC TTTCCAAGTT CTACCGGTAA CCAATTGGAA 2101 TGGTCTGGTG GAAATTTCTT TACCAGCTGC AGCAACACCA ATAATGACGG AAGTACCCCA 2161 ACCTTTATGA CAAGCTTCCA AAGCGTTTCT CATAACCCCA ACATTACCGG TACAATCAAA 2221 AGTGAAATCA CAACCACCAT CGGTCATTTC AATCAATTTA TCGACAATAG TGGTACCTTC 2281 TGGTAATTTG GTTGGGTTAA CAAAGGCAGT GGCACCAAGT TTTTGACCCC ATTCTTCTTT 2341 CTTGTCACTG ATATCAACTA AAATGATTTG AGCAGCTCCT CTTTCAGCAC AACCTTGAAT 2401 GACGGATAAC CCGACAATAC CACCACCGAA AACAGCAACG TTGTCACCTT TTTGAACGTT 2461 GGCGGTGATG GTGGCGGCAC CATAACCAGT AGTAATACCA CAACCTAACA AACAAGCTTT 2521 GTCAAATTCA GCTTTTGGAT TGATGGCAAC AACAGAAATG TCAGCAACAA CAGTATATTG 2581 GGAGAAAGTG GAACAACCCA TAAAGTGGAG AATTTCTTTA CCTTTGCATG TAAATCTTGA 2641 AGTACCATCT GGCATAACAC CTTTACCTTG GGTAGCTCTA ATTTTACCAC ACAAGTTGGT 2701 TTTACCAGAT TTACAGAATT TACATTCACC ACATTCTGGG GTATACAAGG CAATGACATG 2761 GTCACCAACT TTAACATTGG TGACACCTTC ACCAATACTT TCGACAATAC CGGCACCTTC 2821 ATGTCCCAAA ATAACTGGGA AAGCACCTTC TGGATCAACC CCACTTAAAG TGTAAGCATC 2881 AGTGTGACAA ACACCAGTGT CATAAAGTTT GATACGGACT TCATGAGCTT TTGGTGGAGC 2941 AACAGTGACA TCTTCAATGG ATAATGGTTT AGCAGCTTCC CAAGCAACAG CGGCTTTACA 3001 GGTAATTGGC TGAGTAAAGT TAGTAAAATG AACATAATTT CTTTTTTTTG AGTTTATGTT 3061 TTAACCACTA GGGTGATTTT CGAATTGCTC ATGGTGTGTG GGTATAAAAT ATGATGAAAA 3121 TTTCCCTTTT TTTCAAAAAA AAAAAAATTA ACAATTTTCT CCACCAATTG AAAATCATTT 3181 TCCAATTTCA ATTAAGAAAG AACAAATGGA AAAAAAAGGG GGATAAATGA ATTGAGTTAC 3241 ATACTTTTCC AACAGTTGAT TCAGACATTA TTATAGTAAC AATTGATAAA ATAAGTAAGT 3301 TAATTAATCA ATGGTAAGAC TAGAAAATGA AAAGAAGAAG AAGGAGTTGA TTTGGTTTTT 3361 ATAATCTTTT TTTTTTTTTT CTTCGTTCTA TCAGTTTCAT TGAACTTCTT TGTTTCTCGC 3421 GCTGAACGTT GCAGGGAATT GGTTACCCGA TTAGGCTCAT CTTTACGGCT TACTCACGTT 3481 TTTTTTTTTA GTTTCGCATA ATTTTGACCA TGCTTAATAA TTAGGTATCA CAAATAGCGC 3541 GGAAACTTAT CGCTTAAACT CATATAATCA CGAAGTATCT CACCCGCAAT ATTGTTTAGA 3601 AAAGTATTGG TTCTATCTTG ATTACTAATT CTCTATTTTG ATGTAACGTT GACACACTTA 3661 AACTCAGATA TTCTCTTTGT GTTGACTATC GCTTGCTTTT TCCGAAGATA GAATCTTAGA 3721 TCACACTGCC TTTGCTGAGC TGGATCAATA GAGTAACAAA AGAGTGGTAA GGAGCTTGGC 3781 ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG 3841 CCTTGCAGCA CATCCCCCTT TCGCCAGTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC 3901 CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGCGCC TGATGCGGTA TTTTCTCCTT 3961 ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT 4021 GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT 4081 TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT 4141 CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT GATACGCCTA 4201 TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG 4261 GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG 4321 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT 4381 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT 4441 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG 4501 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA 4561 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT 4621 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG 4681 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT 4741 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA 4801 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT 4861 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA 4921 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG 4981 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC 5041 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT 5101 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG 5161 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG 5221 ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA 5281 CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA 5341 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA 5401 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG 5461 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT 5521 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC 5581 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG 5641 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG 5701 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA 5761 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC 5821 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG 5881 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 5941 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC 6001 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT 6061 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC 6121 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC 6181 CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC 6241 AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT 6301 CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG 6361 AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCTTGGCTGC 6421 AGGTCGACGG ATCAAAAATC ATCGCTTCGC TGATTAATTA CCCCAGAAAT AAGGCTAAAA 6481 AACTAATCGC ATTATCATCC TATGGTTGTT AATTTGATTC GTTCATTTGA AGGTTTGTGG 6541 GGCCAGGTTA CTGCCAATTT TTCCTCTTCA TAACCATAAA AGCTAGTATT GTAGAATCTT 6601 TATTGTTCGG AGCAGTGCGG CGCGAGGCAC ATCTGCGTTT CAGGAACGCG ACCGGTGAAG 6661 ACGAGGACGC ACGGAGGAGA GTCTTCCTTC GGAGGGCTGT CACCCGCTCG GCGGCTTCTA 6721 ATCCGTACTT CAATATAGCA ATGAGCAGTT AAGCGTATTA CTGAAAGTTC CAAAGAGAAG 6781 GTTTTTTTAG GCTAATCGGC CTCGAATTCG AGCTCGGTAC CTTTAGTGTC TTCCTAAAAA 6841 AAAAAAAAGG CTCGCCATCA AAACGATATT CGTTGGCTTT TTTTTCTGAA TTATAAATAC 6901 TCTTTGGTAA CTTTTCATTT CCAAGAACCT CTTTTTTCCA GTTATATCAT GGTCCCCTTT 6961 CAAAGTTATT CTCTACTCTT TTTCATATTC ATTCTTTTTC ATCCTTTGGT TTTTTATTCT 7021 TAACTTGTTT ATTATTCTCT CTTGTTTCTA TTTACAAGAC ACCAATCAAA ACAAATAAAA 7081 CATCTCTAGA ATAAACAATG GCTCCAACCA AAAGAAAAGG TGAATGTCCT GGAGCGGCGC 7141 CTAAGAAACC TAAAGAGCCC GTACAAGTAC CCAAGCTGTT GATAAAAGGC GGCGTTGAAG 7201 TGCTGGAGGT TAAAACTGGG GTTGACGCGA TTACAGAAGT TGAATGCTTC CTTAACCCCG 7261 AAATGGGAGA TCCGGATAAT GATCTAAGAG GGTACAGTCT GAGATTAACG GCGGAAACTG 7321 CGTTTGATTC CGACTCACCT GACAGAAAGA TGTTACCTTG TTACAGTACC GCAAGAATCC 7381 CGCTTCCAAA CCTGAACGAG GATTTGACCT GTGGCAACCT TCTTATGTGG GAGGCCGTAA 7441 CTGTAAAAAC GGAAGTAATC GGCATTACAA GTATGCTTAA TCTACATGCG GGATCCCAGA 7501 AGGTGCACGA AAATGGTGGC GGCAAACCTA TACAGGGGAG TAACTTTCAT TTTTTTGCTG 7561 TGGGTGGAGA CCCACTGGAG ATGCAAGGAG TACTTATGAA CTACAGAACG AAGTACCCAG 7621 AGGGAACTGT GACCCCGAAA AATCCGACTG CGCAGTCACA AGTTATGAAC ACGGATCATA 7681 AAGCCTATTT GGACAAGAAC AATGCGTACC CTGTAGAGTG CTGGATACCA GATCCATCAA 7741 AGAACGAAAA TACCCGTTAT TTCGGCACTT ATACGGGCGG CGAGAATGTT CCACCAGTGT 7801 TGCATGTTAC AAACACAGCT ACAACTGTAC TATTAGACGA ACAGGGCGTC GGACCTCTGT 7861 GTAAGGCAGA CTCTTTATAC GTCTCTGCGG CAGATATTTG TGGTTTATTC ACTAATTCAA 7921 GTGGTACCCA GCAGTGGCGT GGCTTGCCAC GTTACTTCAA AATAAGACTT AGGAAAAGGT 7981 CAGTAAAAAA TCCTTACCCA ATCAGCTTCT TGTTATCAGA TTTAATAAAT AGGCGTACTC 8041 AAAGGGTCGA CGGGCAGCCT ATGTACGGGA TGGAGAGCCA AGTGGAAGAG GTTAGGGTTT 8101 TCGATGGGAC AGAACAGCTA CCTGGGGACC CGGATATGAT TAGATATATA GATAGGCAGG 8161 GTCAACTGCA GACTAAAATG GTGTAACAAT GACCCGGGTA CTTAAGGGAT CCAAGGGCCC 8221 AACCTAGGTC TAGATCTCCC ATGTCTCTAC TGGTGGTGGT GCTTCTTTGG AATTATTGGA 8281 AGGTAAGGAA TTGCCAGGTG TTGCTTTCTT ATCCGAAAAG AAATAAATTG AATTGAATTG 8341 AAATCGATAG ATCAATTTTT TTCTTTTCTC TTTCCCCATC CTTTACGCTA AAATAATAGT 8401 TTATTTTATT TTTTGAATAT TTTTTATTTA TATACGTATA TATAGACTAT TATTTATCTT 8461 TTAATGATTA TTAAGATTTT TATTAAAAAA AAATTCGCTC CTCTTTTAAT GCCTTTATGC 8521 AGTTTTTTTT TCCCATTCGA TATTTCTATG TTCGGGTTCA GCGTATTTTA AGTTTAATAA 8581 CTCGAAAATT CTGCGTTCGT TAAAGCTT //