LOCUS       pAsylum                 1766 bp    DNA     circular SYN 17-Jun-2003
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_recomb     139..166
                     /note="Gateway BP Clonase target for removal of bacterial
                     backbone"
                     /note="attB1.1"
     regulatory      173..276
                     /regulatory_class="promoter"
                     /gene="bla"
                     /note="AmpR promoter"
     regulatory      233..238
                     /regulatory_class="CAAT_signal"
     regulatory      257..262
                     /regulatory_class="TATA_box"
     regulatory      274..279
                     /regulatory_class="ribosome_binding_site"
                     /note="Shine-Dalgarno"
     CDS             285..659
                     /note="Zeocin resistance"
                     /product="ShBle"
     3'UTR           660..757
                     /note="pUC57 AmpR 3'UTR"
     regulatory      758..808
                     /regulatory_class="terminator"
                     /note="rrnB T2"
     misc_recomb     809..1040
                     /note="Gateway BP Clonase target for removal of bacterial
                     backbone"
                     /note="attP1"
     3'UTR           1049..1123
                     /note="annotated as Ori in pAsylum"
                     /note="pUC57 AmpR 3' UTR"
     rep_origin      1124..1712
                     /note="MB1 Ori"
     misc_feature    1170..1194
                     /note="Ori hairpin"
ORIGIN
        1 TTCGAAGAAA CATGTTCTTG AATTCAAGAA GAATCGATGT CGTCTTAATT AATCTAGAAA
       61 GAAGGATATC CTACTAAGAT CTAACAACCT CGAGCATCAT AAGCTTCTAC TACGGCCGCA
      121 ACAAAATTAA ATTTTTCAGG GGACAACTTT GTACAAAAAA GTTGGCAAAG TACGCGGAAC
      181 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC
      241 CTGATAAATG CTTCAATAAT ATTGAAAAAG TTAGGAGGTG CATAATGGCC AAGTTGACCA
      301 GTGCTGTCCC AGTGCTCACA GCCAGGGATG TGGCTGGAGC TGTTGAGTTC TGGACTGACA
      361 GGTTGGGGTT CTCCAGAGAT TTTGTGGAGG ATGACTTTGC AGGTGTGGTC AGAGATGATG
      421 TCACCCTGTT CATCTCAGCA GTCCAGGACC AGGTGGTGCC TGACAACACC CTGGCTTGGG
      481 TGTGGGTGAG AGGACTGGAT GAGCTGTATG CTGAGTGGAG TGAGGTGGTG TCCACCAACT
      541 TCAGGGATGC CAGTGGCCCT GCCATGACAG AGATTGGAGA GCAGCCCTGG GGGAGAGAGT
      601 TTGCCCTGAG AGATCCAGCA GGCAACTGTG TGCACTTTGT GGCAGAGGAG CAGGACTGAT
      661 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT
      721 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAA ATTAAGCAGA AGGCCATCCT
      781 GACGGATGGC CTTTTTGCGT TTCTACAAAA ATAATGATTT TATTTTGACT GATAGTGACC
      841 TGTTCGTTGC AACAAATTGA TAAGCAATGC TTTTTTATAA TGCCAACTTT GTACAAAAAA
      901 GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT TTTGCATAAA
      961 AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT CACTATGAAT CAACTACTTA
     1021 GATGGTATTA GTGACCTGTA CCTGCAGGCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT
     1081 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC
     1141 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC
     1201 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC
     1261 CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
     1321 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT
     1381 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT
     1441 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT
     1501 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT
     1561 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG
     1621 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT
     1681 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
     1741 TCCTGGCCTT TTGCTGGCCT TTTGCT
//