LOCUS pMXB10 7592 bp DNA circular SYN 23-Nov-2007 DEFINITION Control vector pMXB10, complete sequence. ACCESSION - KEYWORDS . SOURCE Control vector pMXB10 ORGANISM Control vector pMXB10 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7813) AUTHORS Telenti,A., Southworth,M., Alcaide,F., Daugelat,S., Jacobs,W.R. Jr. and Perler,F.B. TITLE The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein JOURNAL J Bacteriol 179, 6378-6382 (1997) REFERENCE 2 (bases 1 to 7813) AUTHORS Evans,T.C. Jr., Benner,J. and Xu,M.-Q. TITLE Semisynthesis of cytotoxic proteins using a modified protein splicing element JOURNAL Protein Sci 7, 2256-2264 (1998) REFERENCE 3 (bases 1 to 7813) AUTHORS Southworth,M.W., Amaya,K., Evans,T.C., Xu,M.-Q. and Perler,F.B. TITLE Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi Gyrase A intein JOURNAL BioTechniques 27, 110-120 (1999) REFERENCE 4 (bases 1 to 7813) AUTHORS Perler,F.B. TITLE InBase, the intein database JOURNAL Nucleic Acids Res 30 (1), 383-384 (2002) REFERENCE 5 (bases 1 to 7813) AUTHORS Ghosh,I. and Xu,M.Q. TITLE Direct Submission JOURNAL Submitted (23-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers CDS 140..1000 /note="AmpR" sig_peptide 140..208 /note="bla signal peptide" rep_origin 1042..1555 /note="M13 Ori" rep_origin 1666..2254 /note="MB1 Ori" CDS complement(2623..2814) /note="rop" CDS complement(3371..4453) /note="LacI" primer_bind 5637..5656 /note="T7 primer" promoter 5637..5654 /note="T7 promoter (transcript start 5654 clockwise)" protein_bind 5656..5680 /note="LacI binding site" RBS 5710..5716 /note="Shine Dalgarno" misc_recomb 5725..7443 /note="fusion protein weighs 65kD" misc_recomb 5725..6870 /note="cleaved MBP weighs 42 kD" CDS 5725..5742 /note="leader (MCS)" CDS 5743..6840 /note="maltose binding protein (MalE)" CDS 6841..>6852 /note="linker (MCS)" CDS 6853..6873 /note="Tev recognition (ENLYFQ//G)" misc_recomb 6871..7443 /note="cleaved t antigen weighs 22.5kD" CDS 6877..>7434 /note="small t antigen" CDS 6877..7110 /note="T antigen common leader" CDS 7435..7443 /note="Xho1 (LE)" terminator 7464..7586 /note="T7 Tphi transcription terminator" primer_bind 7509..7527 /note="T7 rev primer" BASE COUNT 1941 A 1870 C 2032 G 1749 T 0 OTHER ORIGIN ? 1 AACTACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT 61 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA 121 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT 181 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC 241 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT 301 CCTTGAGAGT TTTCGCCCCG AAGAACGTTC TCCAATGATG AGCACTTTTA AAGTTCTGCT 361 ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA 421 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG 481 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA 541 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 601 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA 661 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 721 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT 781 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG 841 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 901 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA 961 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC 1021 ATATATACTT TAGATTGATT TACCCCGGTT GATAATCAGA AAAGCCCCAA AAACAGGAAG 1081 ATTGTATAAG CAAATATTTA AATTGTAAAC GTTAATATTT TGTTAAAATT CGCGTTAAAT 1141 TTTTGTTAAA TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA 1201 TCAAAAGAAT AGCCCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA 1261 TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA 1321 CTACGTGAAC CATCACCCAA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA AGCACTAAAT 1381 CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG 1441 AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC 1501 ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTAAAAG 1561 GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC 1621 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 1681 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 1741 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 1801 ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 1861 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 1921 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 1981 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 2041 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 2101 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 2161 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 2221 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 2281 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC 2341 TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC 2401 CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 2461 ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC 2521 GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC 2581 CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC 2641 ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GCAGCGATTC 2701 ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT 2761 CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC 2821 CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT 2881 GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA 2941 ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCC 3001 GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT 3061 CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT 3121 TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC 3181 GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT 3241 CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA 3301 GGCTCTCAAG GGCATCGGTC GAGATCCCGG TGCCTAATGA GTGAGCTAAC TTACATTAAT 3361 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG 3421 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCCAGGGTG GTTTTTCTTT 3481 TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC CTGGCCCTGA GAGAGTTGCA 3541 GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC CTGTTTGATG GTGGTTAACG 3601 GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC CACTACCGAG ATATCCGCAC 3661 CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC CAGCGCCATC TGATCGTTGG 3721 CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT TTGCATGGTT TGTTGAAAAC 3781 CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG CTGAATTTGA TTGCGAGTGA 3841 GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC AGAACTTAAT GGGCCCGCTA 3901 ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC CACGCCCAGT CGCGTACCGT 3961 CTTCATGGGA GAAAATAATA CTGTTGATGG GTGTCTGGTC AGAGACATCA AGAAATAACG 4021 CCGGAACATT AGTGCAGGCA GCTTCCACAG CAATGGCATC CTGGTCATCC AGCGGATAGT 4081 TAATGATCAG CCCACTGACG CGTTGCGCGA GAAGATTGTG CACCGCCGCT TTACAGGCTT 4141 CGACGCCGCT TCGTTCTACC ATCGACACCA CCACGCTGGC ACCCAGTTGA TCGGCGCGAG 4201 ATTTAATCGC CGCGACAATT TGCGACGGCG CGTGCAGGGC CAGACTGGAG GTGGCAACGC 4261 CAATCAGCAA CGACTGTTTG CCCGCCAGTT GTTGTGCCAC GCGGTTGGGA ATGTAATTCA 4321 GCTCCGCCAT CGCCGCTTCC ACTTTTTCCC GCGTTTTCGC AGAAACGTGG CTGGCCTGGT 4381 TCACCACGCG GGAAACGGTC TGATAAGAGA CACCGGCATA CTCTGCGACA TCGTATAACG 4441 TTACTGGTTT CACATTCACC ACCCTGAATT GACTCTCTTC CGGGCGCTAT CATGCCATAC 4501 CGCGAAAGGT TTTGCGCCAT TCGATGGTGT CCGGGATCTC GACGCTCTCC CTTATGCGAC 4561 TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG 4621 AATGGTGCAT GCCGGCATGC CGCCCTTTCG TCTTCAAGAA TTAATTCCCA ATTCCCCAGG 4681 CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG 4741 TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG 4801 CAACGGCCCG GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGAA TTAATTCCCC 4861 AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT 4921 TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGG GAGCGGATTT GAACGTTGCG 4981 AAGCAACGGC CCGGAGGGTG GCGGGCAGGA CGCCCGCCAT AAACTGCCAG GAATTAATTC 5041 CCCAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT 5101 TGTTTGTCGG TGAACGCTCT CCTGAGTAGG ACAAATCCGC CGGGAGCGGA TTTGAACGTT 5161 GCGAAGCAAC GGCCCGGAGG GTGGCGGGCA GGACGCCCGC CATAAACTGC CAGGAATTAA 5221 TTCCCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC 5281 TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC CGCCGGGAGC GGATTTGAAC 5341 GTTGCGAAGC AACGGCCCGG AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGAAT 5401 TAATTCCCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT 5461 ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGGG AGCGGATTTG 5521 AACGTTGCGA AGCAACGGCC CGGAGGGTGG CGGGCAGGAC GCCCGCCATA AACTGCCAGG 5581 AATTGGGGAT CGGAATTAAT TCCCGGTTTA AACCGGGGAT CTCGATCCCG CGAAATTAAT 5641 ACGACTCACT ATAGGGGAAT TGTGAGCGGA TAACAATTCC CCTCTAGAAA TAATTTTGTT 5701 TAACTTTAAG AAGGAGATAT ACATATGGGA AGAGCCATGG ATAAAATCGA AGAAGGTAAA 5761 CTGGTAATCT GGATTAACGG CGATAAAGGC TATAACGGTC TCGCTGAAGT CGGTAAGAAA 5821 TTCGAGAAAG ATACCGGAAT TAAAGTCACC GTTGAGCATC CGGATAAACT GGAAGAGAAA 5881 TTCCCACAGG TTGCGGCAAC TGGCGATGGC CCTGACATTA TCTTCTGGGC ACACGACCGC 5941 TTTGGTGGCT ACGCTCAATC TGGCCTGTTG GCTGAAATCA CCCCGGACAA AGCGTTCCAG 6001 GACAAGCTGT ATCCGTTTAC CTGGGATGCC GTACGTTACA ACGGCAAGCT GATTGCTTAC 6061 CCGATCGCTG TTGAAGCGTT ATCGCTGATT TATAACAAAG ATCTGCTGCC GAACCCGCCA 6121 AAAACCTGGG AAGAGATCCC GGCGCTGGAT AAAGAACTGA AAGCGAAAGG TAAGAGCGCG 6181 CTGATGTTCA ACCTGCAAGA ACCGTACTTC ACCTGGCCGC TGATTGCTGC TGACGGGGGT 6241 TATGCGTTCA AGTATGAAAA CGGCAAGTAC GACATTAAAG ACGTGGGCGT GGATAACGCT 6301 GGCGCGAAAG CGGGTCTGAC CTTCCTGGTT GACCTGATTA AAAACAAACA CATGAATGCA 6361 GACACCGATT ACTCCATCGC AGAAGCTGCC TTTAATAAAG GCGAAACAGC GATGACCATC 6421 AACGGCCCGT GGGCATGGTC CAACATCGAC ACCAGCAAAG TGAATTATGG TGTAACGGTA 6481 CTGCCGACCT TCAAGGGTCA ACCATCCAAA CCGTTCGTTG GCGTGCTGAG CGCAGGTATT 6541 AACGCCGCCA GTCCGAACAA AGAGCTGGCA AAAGAGTTCC TCGAAAACTA TCTGCTGACT 6601 GATGAAGGTC TGGAAGCGGT TAATAAAGAC AAACCGCTGG GTGCCGTAGC GCTGAAGTCT 6661 TACGAGGAAG AGTTGGCGAA AGATCCACGT ATTGCCGCCA CTATGGAAAA CGCCCAGAAA 6721 GGTGAAATCA TGCCGAACAT CCCGCAGATG TCCGCTTTCT GGTATGCCGT GCGTACTGCG 6781 GTGATCAACG CCGCCAGCGG TCGTCAGACT GTCGATGAAG CCCTGAAAGA CGCGCAGACT 6841 AATTCGAGCT CGGAAAACCT GTATTTTCAG GGCCATATGG ATTTAGTCCT AAATAGGAAA 6901 GAAAGAGAGG CTCTCTGCAA GCTTTTAGAG ATTGCTCCTA ATTGTTATGG CAACATCCCT 6961 CTGATGAAAG CTGCTTTCAA AAGAAGCTGC TTAAAGCATC ACCCTGATAA AGGGGGAAAT 7021 CCTGTTATAA TGATGGAATT GAACACCCTT TGGAGCAAAT TCCAGCAAAA TATCCACAAG 7081 CTCAGAAGTG ACTTCTCTAT GTTTGATGAG GTCAGTACAA AATTTCCTTG GGAAGAATAT 7141 GGAACTTTAA AGGATTATAT GCAAAGTGGA TATAATGCTA GATTTTGCAG AGGTCCTGGG 7201 TGCATGCTTA AGCAACTTAG AGATTCTAAG TGCGCTTGTA TTAGCTGTAA GTTGTCTCGC 7261 CAGCATTGTA GTCTAAAAAC TTTAAAGCAA AAAAACTGTC TGACGTGGGG AGAGTGTTTT 7321 TGCTATCAGT GCTTTATTCT TTGGTTTGGA TTTCCTCCTA CTTGGGAAAG TTTTGACTGG 7381 TGGCAAAAAA CTTTAGAAGA AACTGACTAC TGCTTACTGC ATCTGCACCT TTTCCTCGAG 7441 TGAGCTGCAG GAAGGGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC 7501 TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG 7561 TTTTTTGCTG AAAGGAGGAA CTATATCCGG AT //