LOCUS pAsylum__ 1894 bp DNA linear SYN 09-May-2018 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers source <1..>48 /note="SV40 enhancer (fragment)" source complement(<188..251) /note="SV40 pA (fragment)" source <557..645 /note="hEF1a polyA signal (fragment)" frag join(1..>73,<107..1894) /date="09-May-2018" /note="1 to 1861 of pAsylum w:INSERT [Split]" primer_bind 24..45 /note="Asylum-R primer" CDS 74..106 /note="Insert 2" CDS <149..>187 /note="INSERT" frag 149..187 /date="03-Aug-2016" /note="16 to 62 of Oligos - pAsylum Plasmid Add In" frag join(188..>1894,1..>73,<107..>148) /date="03-Aug-2016" /note="1 to 1851 of pAsylum[1] [Split]" primer_bind complement(206..227) /note="Asylum-L primer" terminator complement(302..345) /note="rrnB T1 terminator" rRNA complement(349..468) /note="5S rRNA fragment (rrnB operon)" rep_origin 646..1379 /note="MB1 Ori" promoter 1380..1466 /note="EM7 promoter" CDS 1467..1841 /note="Zeo" terminator 1842..1894 /note="rpm B/G terminator" BASE COUNT 455 A 462 C 509 G 468 T 0 OTHER ORIGIN ? 1 CCCTGCAGGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACGC AACTGTTGGG 61 AAGGGCGATC GGTCTCGAGT CATTCGAAGA TGAGCTCAGT CGGCCGGCGG GCCTCTTCGC 121 TATTACGCCA GCCCGGATCC TTATCGATGA GACCGGTTCT GAATTCGAGG GTACCTGCAA 181 CGTTCAGTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA 241 TTGTTGTTGT TCTCCCGGCG GATTTGTCCT ACTCAGGAGA GCGTTCACCG ACAAACAACA 301 GATAAAACGA AAGGCCCAGT CTTTCGACTG AGCCTTTCGT TTTATTTGAT GCCTGGCAGT 361 TCCCTACTCT CGCATGGGGA GACCCCACAC TACCATCGGC GCTACGGCGT TTCACTTCTG 421 AGTTCGGCAT GGGGTCAGGT GGGACCACCG CGCTACTGCC GCCAGGCAAA TTCTGTTTTA 481 TCAGACCGCT TCTGCGTTCT GATTTAATCT GTATCAGGCT GAAAATCTTC TCTCATCCGC 541 CAAAACAGCC AAGCTTCATG TTGCTAAACC AATGTTAAGT GAATCTTTGG AAACAAAATG 601 TTTCCAAATT ACTGGGATGT GCATGTTGAA ACGTGGGTTA ATTAACTAGC CATGACCAAA 661 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA 721 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG 781 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT 841 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC 901 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG 961 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG 1021 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA 1081 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC 1141 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG 1201 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 1261 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC 1321 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTA 1381 ATTAAATTTT TCAAAAGTAG TTGACAATTA ATCATCGGCA TAGTATATCG GCATAGTATA 1441 ATACGACTCA CTATAGGAGG GCCATCATGG CCAAGTTGAC CAGTGCTGTC CCAGTGCTCA 1501 CAGCCAGGGA TGTGGCTGGA GCTGTTGAGT TCTGGACTGA CAGGTTGGGG TTCTCCAGAG 1561 ATTTTGTGGA GGATGACTTT GCAGGTGTGG TCAGAGATGA TGTCACCCTG TTCATCTCAG 1621 CAGTCCAGGA CCAGGTGGTG CCTGACAACA CCCTGGCTTG GGTGTGGGTG AGAGGACTGG 1681 ATGAGCTGTA TGCTGAGTGG AGTGAGGTGG TCTCCACCAA CTTCAGGGAT GCCAGTGGCC 1741 CTGCCATGAC AGAGATTGGA GAGCAGCCCT GGGGGAGAGA GTTTGCCCTG AGAGACCCAG 1801 CAGGCAACTG TGTGCACTTT GTGGCAGAGG AGCAGGACTG AGGATAAGAA TTGTAACAAA 1861 AAACCCCGCC CCGGCGGGGT TTTTTGTTAA TTAA //