LOCUS pGwf 6109 bp DNA circular SYN 17-Jun-2003 DEFINITION . FEATURES Location/Qualifiers misc_feature complement(<49..57) /note="SV40 minor late 19S RNA" promoter <49..>393 /note="SV40 promoter (#SELP)" enhancer 71..213 /note="SV40 enhancer elements" misc_feature 218..281 /note="SV40 21bp repeats" rep_origin 238..>373 /note="SV40 Ori" mRNA 287..293 /note="late-early transcription startpoints" mutation 307 /note="G->A kills SELP ATG" mRNA 327..333 /note="early-early transcription start points" CDS 418..1137 /note="EGFP" polyA_signal 1153..>1374 /note="SV40 Late PolyA" misc_difference 1465..>1656 /note="rhesus-derived" promoter <1465..2643 /note="EF1a promoter and UTR" exon 1663..1695 /note="EF-1a exon 1" intron 1696..2635 /note="EF-1a intron A" exon 2635..2643 /note="EF-1a exon 2 leader" insertion_seq 2667..2691 /note="attB1" CDS 2697..2717 /note="Tev recognition (ENLYFQ//G)" CDS 2721..3473 /note="MCV ALTO (codmod)" CDS 2787..2789 /note="ATG2" CDS 2838..2840 /note="ATG3" CDS 2901..2903 /note="ATG4" CDS 2970..2972 /note="ATG5" CDS 3048..3050 /note="ATG6" CDS 3084..3086 /note="ATG7" misc_recomb 3475..3480 /note="Avr2" misc_recomb 3481..3505 /note="attB2" mRNA 3567..>4155 /note="WPRE" polyA_signal 4191..4860 /note="hEF1a polyA signal" polyA_site 4489 /note="site of polyA addition" CDS 4753 /note="This Sty1 doesn't cut (Gwf Asylum ReX Jan2010)" rep_origin 4861..5594 /note="MB1 Ori" promoter 5595..5681 /note="EM7 promoter" CDS 5682..6056 /note="Zeo" terminator 6057..6109 /note="terminator (rpmB/G)" BASE COUNT 1388 A 1660 C 1638 G 1422 T 1 OTHER ORIGIN 1 CCCTGCAGGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT GTGGAATGTG 61 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 121 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 181 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 241 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 301 ATTTATACAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 361 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTTGATTGGG ATCCACCGGT CGCCACCATG 421 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC 481 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC 541 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC 601 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG 661 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC 721 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG 781 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG 841 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC 901 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC 961 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC 1021 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG 1081 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC 1141 GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA 1201 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA 1261 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC 1321 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG 1381 ATAAGGATCC GGGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT 1441 TGCGGTGGAG AAGAGCATGC GTGAGGCTCC GGTGCCCGTC AGTGGGCAGA GCGCACATCG 1501 CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACCGGTGC CTAGAGAAGG 1561 TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCCTTT TCCCGAGGGT 1621 GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCTGTGAACG TTCTTTTTCG CAACGGGTTT 1681 GCCGCCAGAA CACAGGTAAG TGCCGTGTGT GGTTCCCGCG GGCCTGGCCT CTTTACGGGT 1741 TATGGCCCTT GCGTGCCTTG AATTACTTCC ACCTGGCTGC AGTACGTGAT TCTTGATCCC 1801 GAGCTTCGGG TTGGAAGTGG GTGGGAGAGT TCGAGGCCTT GCGCTTAAGG AGCCCCTTCG 1861 CCTCGTGCTT GAGTTGAGGC CTGGCCTGGG CGCTGGGGCC GCCGCGTGCG AATCTGGTGG 1921 CACCTTCGCG CCTGTCTCGC TGCTTTCGAT AAGTCTCTAG CCATTTAAAA TTTTTGATGA 1981 CCTGCTGCGA CGCTTTTTTT CTGGCAAGAT AGTCTTGTAA ATGCGGGCCA AGATCTGCAC 2041 ACTGGTATTT CGGTTTTTGG GGCCGCGGGC GGCGACGGGG CCCGTGCGTC CCAGCGCACA 2101 TGTTCGGCGA GGCGGGGCCT GCGAGCGCGG CCACCGAGAA TCGGACGGGG GTAGTCTCAA 2161 GCTGGCCGGC CTGCTCTGGT GCCTGGCCTC GCGCCGCCGT GTATCGCCCC GCCCTGGGCG 2221 GCAAGGCTGG CCCGGTCGGC ACCAGTTGCG TGAGCGGAAA GATGGCCGCT TCCCGGCCCT 2281 GCTGCAGGGA GCTCAAAATG GAGGACGCGG CGCTCGGGAG AGCGGGCGGG TGAGTCACCC 2341 ACACAAAGGA AAAGGGCCTT TCCGTCCTCA GCCGTCGCTT CATGTGACTC CACGGAGTAC 2401 CGGGCGCCGT CCAGGCACCT CGATTAGTTC TCGAGCTTTT GGAGTACGTC GTCTTTAGGT 2461 TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA GACTGAAGTT 2521 AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT 2581 TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG 2641 TGAGGAATTC TCTAGAGCTT GATCAAACAA GTTTGTACAA AAAAGCAGGC TCCGGAGAAA 2701 ACCTGTACTT TCAAGGCGCC ATGGGCCCAC TGAACAGCAA GAACGGAGGC GACCAAGAAG 2761 ACAGCGCCAG CGGACGCCAC ACCAACATGG GACCCATTCA TACCGGACCC ACCCAGGACC 2821 CCGAGTCATT GCCACCCATG CACCCTGGCG AACCTCCGGT CGAGGCCCAC CATCCAACCG 2881 CACGCGCACT GCCCCTCGGC ATGGGCCCAA GCCAAAGGCC ACGCTTGCAA ACCCCAAGCC 2941 CCGAAGATCC CATCTATCTG CCCAATACCA TGCGCAATCC ACCTCACCCA CTCGACCCAG 3001 TGGCCGAACG CCGCCCACCT ATCCAAGAGG AAAACCCAGC CCATCCAATG GAGCCCGTCT 3061 ATCTGGAAAT CTTGCCTGAG AGAATGGCAC CAGGACGCAT TAGCTCCGCC ATGAACCATT 3121 TCCCACCACT CTCACTCCCA AGGCCACTCC GTTCGCTGCG CAGCCCACCG CCACAAGAGG 3181 CCAGGCCAGG GTCACCAAGG TTGCCTCTGC CACGCCGCCC AAGGCACCTG TCCCTGCAAA 3241 TGCGCAACAC CGACCCGCCA CCAAGCCCAC CAAGGCGCCC ACTGTTGCAC AGCCAGGAGT 3301 CCGAGAACCT CGGCGGACCA GAGGCACTCC AGGCCCTGCT CGTGCAACAG GTGCTGCAGG 3361 CACTGCATCA GTCACAGAAG CGCACCGAAA AGTTGCTGTT TCTGCTCATC TTCCTGCTGA 3421 TCTTCCTGAT CATTCTGGCA ATGCTCTACA TCGTTATCAA GCAACAGATC TGAGCCTAGG 3481 ACCCAGCTTT CTTGTACAAA GTGGTTCGAT CTAGAATGGC TAGTGGATCC CCCGGGCTGC 3541 AGGAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT 3601 TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC 3661 CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT 3721 GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA 3781 CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT 3841 CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG 3901 CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA 3961 AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT 4021 CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC 4081 CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT 4141 GGGCCGCCTC CCCGCATCGA TACCGTCGGC CCACTGCTCC CTAAACCTGA GCTAGCATTA 4201 TCCCTAATAC CTGCCACCCC ACTCTTAATC AGTGGTGGAA GAACGGTCTC AGAACTGTTT 4261 GTTTCAATTG GCCATTTAAG TTTAGTAGTA AAAGACTGGT TAATGATAAC AATGCATCGT 4321 AAAACCTTCA GAAGGAAAGG AGAATGTTTT GTGGACCACT TTGGTTTTCT TTTTTGCGTG 4381 TGGCAGTTTT AAGTTATTAG TTTTTAAAAT CAGTACTTTT TAATGGAAAC AACTTGACCA 4441 AAAATTTGTC ACAGAATTTT GAGACCCATT AAAAAAGTTA AATGAGAAAC CTGTGTGTTC 4501 CTTTGGTCAA CACCGAGACA TTTAGGTGAA AGACATCTAA TTCTGGTTTT ACGAATCTGG 4561 AAACTTCTTG AAAATGTAAT TCTTGAGTTA ACACTTCTGG GTGGAGAATA GGGTTGTTTT 4621 CCCCCCACAT AATTGGAAGG GGAAGGAATA TCATTTAAAG CTATGGGAGG GTTTCTTTGA 4681 TTACAACACT GGAGAGAAAT GCAGCATGTT GCTGATTGCC TGTCACTAAA ACAGGCCAAA 4741 AACTGAGTCC TTNGGGTTGC ATAGAAAGCT TCATGTTGCT AAACCAATGT TAAGTGAATC 4801 TTTGGAAACA AAATGTTTCC AAATTACTGG GATGTGCATG TTGAAACGTG GGTTAATTAA 4861 CTAGCCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA 4921 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA 4981 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT 5041 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG 5101 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA 5161 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA 5221 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG 5281 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA 5341 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA 5401 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC 5461 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC 5521 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT 5581 GCTCACATGT TCTTAATTAA ATTTTTCAAA AGTAGTTGAC AATTAATCAT CGGCATAGTA 5641 TATCGGCATA GTATAATACG ACTCACTATA GGAGGGCCAT CATGGCCAAG TTGACCAGTG 5701 CTGTCCCAGT GCTCACAGCC AGGGATGTGG CTGGAGCTGT TGAGTTCTGG ACTGACAGGT 5761 TGGGGTTCTC CAGAGATTTT GTGGAGGATG ACTTTGCAGG TGTGGTCAGA GATGATGTCA 5821 CCCTGTTCAT CTCAGCAGTC CAGGACCAGG TGGTGCCTGA CAACACCCTG GCTTGGGTGT 5881 GGGTGAGAGG ACTGGATGAG CTGTATGCTG AGTGGAGTGA GGTGGTCTCC ACCAACTTCA 5941 GGGATGCCAG TGGCCCTGCC ATGACAGAGA TTGGAGAGCA GCCCTGGGGG AGAGAGTTTG 6001 CCCTGAGAGA CCCAGCAGGC AACTGTGTGC ACTTTGTGGC AGAGGAGCAG GACTGAGGAT 6061 AAGAATTGTA ACAAAAAACC CCGCCCCGGC GGGGTTTTTT GTTAATTAA //