LOCUS pGwf 6195 bp DNA circular SYN 17-Jun-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers misc_feature complement(<49..57) /note="SV40 minor late 19S RNA" promoter <49..>393 /note="SV40 promoter ( SELP)" enhancer 71..213 /note="SV40 enhancer elements" misc_feature 218..281 /note="SV40 21bp repeats" rep_origin 238..>373 /note="SV40 Ori" mRNA 287..293 /note="late-early transcription startpoints" mutation 307 /note="G->A kills SELP ATG" mRNA 327..333 /note="early-early transcription start points" CDS 418..1137 /note="EGFP" polyA_signal 1153..>1374 /note="SV40 Late PolyA" mutation 1444 /note="missing A (h Lsf sequencing)" misc_difference 1465..>1656 /note="rhesus-derived" promoter <1465..2643 /note="EF1a promoter and UTR" exon 1663..1695 /note="EF-1a exon 1" intron 1696..2635 /note="EF-1a intron A" primer_bind 2625..2644 /note="a1179R" exon 2635..2643 /note="EF-1a exon 2 leader" CDS 2678..3055 /note="H2B (HIST1H2BJ)" CDS 3056..3088 /note="linker" CDS 3089..3106 /note="His tag" CDS 3107..3121 /note="Linker" CDS 3122..3634 /note="NanoLuc" primer_bind complement(3168..3186) /note="NwRev sequencing primer" mRNA 3653..4241 /note="WPRE" polyA_signal 4277..4946 /note="hEF1a polyA signal" polyA_site 4575 /note="site of polyA addition" CDS 4839 /note="This Sty1 doesn't cut (Gwf Asylum ReX Jan2010)" rep_origin 4947..5680 /note="MB1 Ori" promoter 5681..5767 /note="EM7 promoter" CDS 5768..6142 /note="Zeo" terminator 6143..6195 /note="terminator (rpmB/G)" BASE COUNT 1404 A 1609 C 1714 G 1467 T 1 OTHER ORIGIN ? 1 CCCTGCAGGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT GTGGAATGTG 61 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 121 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 181 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 241 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 301 ATTTATACAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 361 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTTGATTGGG ATCCACCGGT CGCCACCATG 421 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC 481 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC 541 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC 601 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG 661 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC 721 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG 781 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG 841 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC 901 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC 961 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC 1021 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG 1081 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC 1141 GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA 1201 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA 1261 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC 1321 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG 1381 ATAAGGATCC GGGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT 1441 TGCGGTGGAG AAGAGCATGC GTGAGGCTCC GGTGCCCGTC AGTGGGCAGA GCGCACATCG 1501 CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACCGGTGC CTAGAGAAGG 1561 TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCCTTT TCCCGAGGGT 1621 GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCTGTGAACG TTCTTTTTCG CAACGGGTTT 1681 GCCGCCAGAA CACAGGTAAG TGCCGTGTGT GGTTCCCGCG GGCCTGGCCT CTTTACGGGT 1741 TATGGCCCTT GCGTGCCTTG AATTACTTCC ACCTGGCTGC AGTACGTGAT TCTTGATCCC 1801 GAGCTTCGGG TTGGAAGTGG GTGGGAGAGT TCGAGGCCTT GCGCTTAAGG AGCCCCTTCG 1861 CCTCGTGCTT GAGTTGAGGC CTGGCCTGGG CGCTGGGGCC GCCGCGTGCG AATCTGGTGG 1921 CACCTTCGCG CCTGTCTCGC TGCTTTCGAT AAGTCTCTAG CCATTTAAAA TTTTTGATGA 1981 CCTGCTGCGA CGCTTTTTTT CTGGCAAGAT AGTCTTGTAA ATGCGGGCCA AGATCTGCAC 2041 ACTGGTATTT CGGTTTTTGG GGCCGCGGGC GGCGACGGGG CCCGTGCGTC CCAGCGCACA 2101 TGTTCGGCGA GGCGGGGCCT GCGAGCGCGG CCACCGAGAA TCGGACGGGG GTAGTCTCAA 2161 GCTGGCCGGC CTGCTCTGGT GCCTGGCCTC GCGCCGCCGT GTATCGCCCC GCCCTGGGCG 2221 GCAAGGCTGG CCCGGTCGGC ACCAGTTGCG TGAGCGGAAA GATGGCCGCT TCCCGGCCCT 2281 GCTGCAGGGA GCTCAAAATG GAGGACGCGG CGCTCGGGAG AGCGGGCGGG TGAGTCACCC 2341 ACACAAAGGA AAAGGGCCTT TCCGTCCTCA GCCGTCGCTT CATGTGACTC CACGGAGTAC 2401 CGGGCGCCGT CCAGGCACCT CGATTAGTTC TCGAGCTTTT GGAGTACGTC GTCTTTAGGT 2461 TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA GACTGAAGTT 2521 AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT 2581 TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG 2641 TGAGGAATTC TCTAGAGCAG TCGACGGTAC CGCCACCATG CCAGAGCCAG CGAAGTCTGC 2701 TCCCGCCCCG AAAAAGGGCT CCAAGAAGGC GGTGACTAAG GCGCAGAAGA AAGACGGCAA 2761 GAAGCGCAAG CGCAGCCGCA AGGAGAGCTA TTCCATCTAT GTGTACAAGG TTCTGAAGCA 2821 GGTCCACCCT GACACCGGCA TTTCGTCCAA GGCCATGGGC ATCATGAATT CGTTTGTGAA 2881 CGACATTTTC GAGCGCATCG CAGGTGAGGC TTCCCGCCTG GCGCATTACA ACAAGCGCTC 2941 GACCATCACC TCCAGGGAGA TCCAGACGGC CGTGCGCCTG CTGCTGCCTG GGGAGTTGGC 3001 CAAGCACGCC GTGTCCGAGG GTACTAAGGC CGTCACCAAG TATACCAGTG CTAAGGGAGC 3061 ATCGGATCCT AGTGCTTCGA ACCCCGGGCA CCACCATCAC CACCATGCCG GCAGCGGCAC 3121 CGTCTTCACA CTCGAAGATT TCGTTGGGGA CTGGCGACAG ACAGCCGGCT ACAACCTGGA 3181 CCAAGTCCTT GAACAGGGAG GTGTGTCCAG TTTGTTTCAG AATCTCGGGG TGTCCGTAAC 3241 TCCGATCCAA AGGATTGTCC TGAGCGGTGA AAATGGGCTG AAGATCGACA TCCATGTCAT 3301 CATCCCGTAT GAAGGTCTGA GCGGCGACCA AATGGGCCAG ATCGAAAAAA TTTTTAAGGT 3361 GGTGTACCCT GTGGATGATC ATCACTTTAA GGTGATCCTG CACTATGGCA CACTGGTAAT 3421 CGACGGGGTT ACGCCGAACA TGATCGACTA TTTCGGACGG CCGTATGAAG GCATCGCCGT 3481 GTTCGACGGC AAAAAGATCA CTGTAACAGG GACCCTGTGG AACGGCAACA AAATTATCGA 3541 CGAGCGCCTG ATCAACCCCG ACGGCTCCCT GCTGTTCCGA GTAACCATCA ACGGAGTGAC 3601 CGGCTGGCGG CTGTGCGAAC GCATTCTGGC GTGAGATATC AAGCTTATCG ATAATCAACC 3661 TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC 3721 GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT 3781 CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT 3841 TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG 3901 CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC 3961 GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC 4021 TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT 4081 TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC 4141 GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG 4201 CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CATCGATACC GTCGGCCCAC 4261 TGCTCCCTAA ACCTGAGCTA GCATTATCCC TAATACCTGC CACCCCACTC TTAATCAGTG 4321 GTGGAAGAAC GGTCTCAGAA CTGTTTGTTT CAATTGGCCA TTTAAGTTTA GTAGTAAAAG 4381 ACTGGTTAAT GATAACAATG CATCGTAAAA CCTTCAGAAG GAAAGGAGAA TGTTTTGTGG 4441 ACCACTTTGG TTTTCTTTTT TGCGTGTGGC AGTTTTAAGT TATTAGTTTT TAAAATCAGT 4501 ACTTTTTAAT GGAAACAACT TGACCAAAAA TTTGTCACAG AATTTTGAGA CCCATTAAAA 4561 AAGTTAAATG AGAAACCTGT GTGTTCCTTT GGTCAACACC GAGACATTTA GGTGAAAGAC 4621 ATCTAATTCT GGTTTTACGA ATCTGGAAAC TTCTTGAAAA TGTAATTCTT GAGTTAACAC 4681 TTCTGGGTGG AGAATAGGGT TGTTTTCCCC CCACATAATT GGAAGGGGAA GGAATATCAT 4741 TTAAAGCTAT GGGAGGGTTT CTTTGATTAC AACACTGGAG AGAAATGCAG CATGTTGCTG 4801 ATTGCCTGTC ACTAAAACAG GCCAAAAACT GAGTCCTTNG GGTTGCATAG AAAGCTTCAT 4861 GTTGCTAAAC CAATGTTAAG TGAATCTTTG GAAACAAAAT GTTTCCAAAT TACTGGGATG 4921 TGCATGTTGA AACGTGGGTT AATTAACTAG CCATGACCAA AATCCCTTAA CGTGAGTTTT 4981 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT 5041 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT 5101 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA 5161 TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG 5221 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA 5281 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG 5341 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA 5401 GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA 5461 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA 5521 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT 5581 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC 5641 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT AATTAAATTT TTCAAAAGTA 5701 GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACTC ACTATAGGAG 5761 GGCCATCATG GCCAAGTTGA CCAGTGCTGT CCCAGTGCTC ACAGCCAGGG ATGTGGCTGG 5821 AGCTGTTGAG TTCTGGACTG ACAGGTTGGG GTTCTCCAGA GATTTTGTGG AGGATGACTT 5881 TGCAGGTGTG GTCAGAGATG ATGTCACCCT GTTCATCTCA GCAGTCCAGG ACCAGGTGGT 5941 GCCTGACAAC ACCCTGGCTT GGGTGTGGGT GAGAGGACTG GATGAGCTGT ATGCTGAGTG 6001 GAGTGAGGTG GTCTCCACCA ACTTCAGGGA TGCCAGTGGC CCTGCCATGA CAGAGATTGG 6061 AGAGCAGCCC TGGGGGAGAG AGTTTGCCCT GAGAGACCCA GCAGGCAACT GTGTGCACTT 6121 TGTGGCAGAG GAGCAGGACT GAGGATAAGA ATTGTAACAA AAAACCCCGC CCCGGCGGGG 6181 TTTTTTGTTA ATTAA //