LOCUS pMLO7w 6949 bp DNA linear SYN 22-May-2017 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers misc_feature complement(49..57) /note="SV40 minor late 19S RNA" misc_feature 218..281 /note="SV40 21bp repeats" rep_origin 238..373 /note="SV40 Ori" mRNA 287..293 /note="late-early transcription startpoints" mRNA 327..333 /note="early-early transcription start points" CDS 418..1137 /note="EGFP" exon 1663..1695 /note="EF-1a exon 1" intron 1696..2635 /note="EF-1a intron A" exon 2635..2643 /note="EF-1a exon 2 leader" CDS 2697..2717 /note="Tev recognition (ENLYFQ//G)" CDS 2721..4314 /note="Marbled Eel LO7" mRNA 4407..4995 /note="WPRE" polyA_site 5329 /note="site of polyA addition" rep_origin 5701..6434 /note="MB1 Ori" CDS 6522..6896 /note="Zeo" BASE COUNT 1606 A 1897 C 1834 G 1611 T 1 OTHER ORIGIN ? 1 CCCTGCAGGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT GTGGAATGTG 61 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 121 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 181 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 241 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 301 ATTTATACAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 361 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTTGATTGGG ATCCACCGGT CGCCACCATG 421 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC 481 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC 541 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC 601 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG 661 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC 721 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG 781 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG 841 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC 901 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC 961 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC 1021 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG 1081 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC 1141 GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA 1201 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA 1261 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC 1321 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG 1381 ATAAGGATCC GGGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT 1441 TGCGGTGGAG AAGAGCATGC GTGAGGCTCC GGTGCCCGTC AGTGGGCAGA GCGCACATCG 1501 CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACCGGTGC CTAGAGAAGG 1561 TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCCTTT TCCCGAGGGT 1621 GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCTGTGAACG TTCTTTTTCG CAACGGGTTT 1681 GCCGCCAGAA CACAGGTAAG TGCCGTGTGT GGTTCCCGCG GGCCTGGCCT CTTTACGGGT 1741 TATGGCCCTT GCGTGCCTTG AATTACTTCC ACCTGGCTGC AGTACGTGAT TCTTGATCCC 1801 GAGCTTCGGG TTGGAAGTGG GTGGGAGAGT TCGAGGCCTT GCGCTTAAGG AGCCCCTTCG 1861 CCTCGTGCTT GAGTTGAGGC CTGGCCTGGG CGCTGGGGCC GCCGCGTGCG AATCTGGTGG 1921 CACCTTCGCG CCTGTCTCGC TGCTTTCGAT AAGTCTCTAG CCATTTAAAA TTTTTGATGA 1981 CCTGCTGCGA CGCTTTTTTT CTGGCAAGAT AGTCTTGTAA ATGCGGGCCA AGATCTGCAC 2041 ACTGGTATTT CGGTTTTTGG GGCCGCGGGC GGCGACGGGG CCCGTGCGTC CCAGCGCACA 2101 TGTTCGGCGA GGCGGGGCCT GCGAGCGCGG CCACCGAGAA TCGGACGGGG GTAGTCTCAA 2161 GCTGGCCGGC CTGCTCTGGT GCCTGGCCTC GCGCCGCCGT GTATCGCCCC GCCCTGGGCG 2221 GCAAGGCTGG CCCGGTCGGC ACCAGTTGCG TGAGCGGAAA GATGGCCGCT TCCCGGCCCT 2281 GCTGCAGGGA GCTCAAAATG GAGGACGCGG CGCTCGGGAG AGCGGGCGGG TGAGTCACCC 2341 ACACAAAGGA AAAGGGCCTT TCCGTCCTCA GCCGTCGCTT CATGTGACTC CACGGAGTAC 2401 CGGGCGCCGT CCAGGCACCT CGATTAGTTC TCGAGCTTTT GGAGTACGTC GTCTTTAGGT 2461 TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA GACTGAAGTT 2521 AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT 2581 TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG 2641 TGAGGAATTC TCTAGAGCTT GATCAAACAA GTTTGTACAA AAAAGCAGGC TCCGGAGAAA 2701 ACCTGTACTT TCAAGGCACC ATGGCAACAA ACAGAGCCGA CGACGCCTAC GACGAAGATC 2761 TCAGCCTCAC ACTCCAGCAG AATCTGACCT CATACCACGT CGCCGGGCCA TTGACAACCC 2821 AAGTCTATAC CCGCGAAGGC CAAAGCCACG GAGGCCCATC ACTCGTGACA CAGCTGAATC 2881 AGCAGGGGAC CGGCGATTGC GCCATCGATC TGCAGTGTCC CGTGGATGAA GCCATTCTGG 2941 TCGGCTCCAT CAGCTTCGTC GCCAGAGGCC ACGTCGTCAG TTACAACGCC GCAGGCGTGC 3001 TCCAAGCCGG CTGGCAATCA ACCGACCACA ATGGCGCCAA CTGGGCCCAA CTCAACGCCA 3061 ATCAAGCAAA CCAAGCCTCA AGAGTGTCCA TGCCATTCTT CTTTTTGAAT AACCAATTCA 3121 CCCAAATCGA TCTCGAGATC GGCCAGACCC AGTCCGTGAA TGCCTATCTG CCCTTGACCC 3181 GCAGCTCAGA ATTTGGACCC GGCCCAATTG GCATCCTGAA CCAGTATTTC AATCAACCAA 3241 GTGACGCCGG CTACTATCAC GAGAACAGTA CCTCCCATAC AAGCAGCGCC ACCCATTTGA 3301 CCCAATGTAG CGTCGCCGCC AATACCGATT TCTGGGTCGC CCAAGAACGC GTGGTCCACC 3361 AATTGAACAC AACCCACAAC CTGCTCAGCA GCTGGGATTT CGACGATCAT GAGGATAGGC 3421 GCATTTTGCC CCGCGGAATG AATCTCAGGA TTAGAGGCTG TACACGCCCC TACAATGACC 3481 GCAGCATCGT CGCCATGAGC CATAATGCAA ATGCCCAACA GACCTTGCGC TTGGTCTTCG 3541 ATTCCATGAG GGTCATCTAT ACAACCGTGA AGATCCCACA CAGCCACCGC TCCAGCGAGA 3601 CCATTGAGAG CCTGTTCGCA ACAATCGATA CCGGCCTCAG GAGCCATGCA TTGGTCCAGG 3661 GCCAACGCAG CGCCACCATC CCCATCACCC ATACCGATGC CGATATCGTG CCCGGCGTGA 3721 TCGTCCTGTT CGTCGCCGAT AACAACGCCT TCAATACAGT CAATTTGACC CAGACCCCCA 3781 GTGTCATCAC AAGCGTCCCA AACATTATCA CATCCGTCCG CTGTAACTTG AACGGCAACG 3841 GGATGCCCGA TTTCATGAGC ATCTATATGG AAAGCGTCTG TAACCCAACC GATATGTTGC 3901 ATCGCTGCGC CATGATTAAC GCCTTCTTGG GGCGCGCAGG CAATATGACA GGCCGCTCAC 3961 GCCAAGGCGA TCGCAGCCCC GGCGAAACCC ATTTGATGAG CTATACCGCC AGGGACGCAG 4021 CCAACGCCGC CTCCGTCCTG GCCATTAACG CCGTGATCTT GTGTACCGAT CCATCAACAA 4081 CCGTCGTCCG CGAAGCAGGC AGTGGCGTCT TGACCGGCAG ACTCGATGTC CAGATCGAGC 4141 TGGGGCAGAA CATCCTCAAC ACCCATCGCA TCTATGCCCT CAGTTTTTGC AAGCAACAAA 4201 TCGGCATCGG CAAAGTCCGC GAGCAAGATG GCACCCAGCC CCAATATGTC TTGTCCGTCT 4261 CCGAGACACG CCCCGCCTAT ACCAGGGTCA CCGTCGAAGA GATGAGTTAT TGAGCCTAGG 4321 ACCCAGCTTT CTTGTACAAA GTGGTTCGAT CTAGAATGGC TAGTGGATCC CCCGGGCTGC 4381 AGGAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT 4441 TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC 4501 CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT 4561 GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA 4621 CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT 4681 CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG 4741 CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA 4801 AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT 4861 CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC 4921 CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT 4981 GGGCCGCCTC CCCGCATCGA TACCGTCGGC CCACTGCTCC CTAAACCTGA GCTAGCATTA 5041 TCCCTAATAC CTGCCACCCC ACTCTTAATC AGTGGTGGAA GAACGGTCTC AGAACTGTTT 5101 GTTTCAATTG GCCATTTAAG TTTAGTAGTA AAAGACTGGT TAATGATAAC AATGCATCGT 5161 AAAACCTTCA GAAGGAAAGG AGAATGTTTT GTGGACCACT TTGGTTTTCT TTTTTGCGTG 5221 TGGCAGTTTT AAGTTATTAG TTTTTAAAAT CAGTACTTTT TAATGGAAAC AACTTGACCA 5281 AAAATTTGTC ACAGAATTTT GAGACCCATT AAAAAAGTTA AATGAGAAAC CTGTGTGTTC 5341 CTTTGGTCAA CACCGAGACA TTTAGGTGAA AGACATCTAA TTCTGGTTTT ACGAATCTGG 5401 AAACTTCTTG AAAATGTAAT TCTTGAGTTA ACACTTCTGG GTGGAGAATA GGGTTGTTTT 5461 CCCCCCACAT AATTGGAAGG GGAAGGAATA TCATTTAAAG CTATGGGAGG GTTTCTTTGA 5521 TTACAACACT GGAGAGAAAT GCAGCATGTT GCTGATTGCC TGTCACTAAA ACAGGCCAAA 5581 AACTGAGTCC TTNGGGTTGC ATAGAAAGCT TCATGTTGCT AAACCAATGT TAAGTGAATC 5641 TTTGGAAACA AAATGTTTCC AAATTACTGG GATGTGCATG TTGAAACGTG GGTTAATTAA 5701 CTAGCCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA 5761 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA 5821 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT 5881 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG 5941 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA 6001 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA 6061 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG 6121 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA 6181 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA 6241 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC 6301 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC 6361 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT 6421 GCTCACATGT TCTTAATTAA ATTTTTCAAA AGTAGTTGAC AATTAATCAT CGGCATAGTA 6481 TATCGGCATA GTATAATACG ACTCACTATA GGAGGGCCAT CATGGCCAAG TTGACCAGTG 6541 CTGTCCCAGT GCTCACAGCC AGGGATGTGG CTGGAGCTGT TGAGTTCTGG ACTGACAGGT 6601 TGGGGTTCTC CAGAGATTTT GTGGAGGATG ACTTTGCAGG TGTGGTCAGA GATGATGTCA 6661 CCCTGTTCAT CTCAGCAGTC CAGGACCAGG TGGTGCCTGA CAACACCCTG GCTTGGGTGT 6721 GGGTGAGAGG ACTGGATGAG CTGTATGCTG AGTGGAGTGA GGTGGTCTCC ACCAACTTCA 6781 GGGATGCCAG TGGCCCTGCC ATGACAGAGA TTGGAGAGCA GCCCTGGGGG AGAGAGTTTG 6841 CCCTGAGAGA CCCAGCAGGC AACTGTGTGC ACTTTGTGGC AGAGGAGCAG GACTGAGGAT 6901 AAGAATTGTA ACAAAAAACC CCGCCCCGGC GGGGTTTTTT GTTAATTAA //