LOCUS pMLO2-3w 7056 bp DNA linear SYN 22-May-2017 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers misc_feature complement(49..57) /note="SV40 minor late 19S RNA" misc_feature 218..281 /note="SV40 21bp repeats" rep_origin 238..373 /note="SV40 Ori" mRNA 287..293 /note="late-early transcription startpoints" mRNA 327..333 /note="early-early transcription start points" CDS 418..1137 /note="EGFP" exon 1663..1695 /note="EF-1a exon 1" intron 1696..2635 /note="EF-1a intron A" exon 2635..2643 /note="EF-1a exon 2 leader" CDS 2703..4421 /note="MAdO LO2-3" mRNA 4514..5102 /note="WPRE" polyA_site 5436 /note="site of polyA addition" rep_origin 5808..6541 /note="MB1 Ori" CDS 6629..7003 /note="Zeo" BASE COUNT 1613 A 1987 C 1882 G 1573 T 1 OTHER ORIGIN ? 1 CCCTGCAGGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT GTGGAATGTG 61 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 121 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 181 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 241 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 301 ATTTATACAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 361 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTTGATTGGG ATCCACCGGT CGCCACCATG 421 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC 481 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC 541 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC 601 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG 661 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC 721 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG 781 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG 841 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC 901 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC 961 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC 1021 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG 1081 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC 1141 GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA 1201 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA 1261 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC 1321 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG 1381 ATAAGGATCC GGGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT 1441 TGCGGTGGAG AAGAGCATGC GTGAGGCTCC GGTGCCCGTC AGTGGGCAGA GCGCACATCG 1501 CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACCGGTGC CTAGAGAAGG 1561 TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCCTTT TCCCGAGGGT 1621 GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCTGTGAACG TTCTTTTTCG CAACGGGTTT 1681 GCCGCCAGAA CACAGGTAAG TGCCGTGTGT GGTTCCCGCG GGCCTGGCCT CTTTACGGGT 1741 TATGGCCCTT GCGTGCCTTG AATTACTTCC ACCTGGCTGC AGTACGTGAT TCTTGATCCC 1801 GAGCTTCGGG TTGGAAGTGG GTGGGAGAGT TCGAGGCCTT GCGCTTAAGG AGCCCCTTCG 1861 CCTCGTGCTT GAGTTGAGGC CTGGCCTGGG CGCTGGGGCC GCCGCGTGCG AATCTGGTGG 1921 CACCTTCGCG CCTGTCTCGC TGCTTTCGAT AAGTCTCTAG CCATTTAAAA TTTTTGATGA 1981 CCTGCTGCGA CGCTTTTTTT CTGGCAAGAT AGTCTTGTAA ATGCGGGCCA AGATCTGCAC 2041 ACTGGTATTT CGGTTTTTGG GGCCGCGGGC GGCGACGGGG CCCGTGCGTC CCAGCGCACA 2101 TGTTCGGCGA GGCGGGGCCT GCGAGCGCGG CCACCGAGAA TCGGACGGGG GTAGTCTCAA 2161 GCTGGCCGGC CTGCTCTGGT GCCTGGCCTC GCGCCGCCGT GTATCGCCCC GCCCTGGGCG 2221 GCAAGGCTGG CCCGGTCGGC ACCAGTTGCG TGAGCGGAAA GATGGCCGCT TCCCGGCCCT 2281 GCTGCAGGGA GCTCAAAATG GAGGACGCGG CGCTCGGGAG AGCGGGCGGG TGAGTCACCC 2341 ACACAAAGGA AAAGGGCCTT TCCGTCCTCA GCCGTCGCTT CATGTGACTC CACGGAGTAC 2401 CGGGCGCCGT CCAGGCACCT CGATTAGTTC TCGAGCTTTT GGAGTACGTC GTCTTTAGGT 2461 TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA GACTGAAGTT 2521 AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT 2581 TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG 2641 TGAGGAATTC TCTAGAGCTT GATCAAACAA GTTTGTACAA AAAAGCAGGC TCCGGAGCCA 2701 CCATGTGGCA CGTTTGCTGT TCGACCATGC TACGCAAGAG GAGACGCAGG AACCACAAGA 2761 GCAAGGCCGT CAACGTCAAG GCCGAGCCCT CGACGACCTC ACTGCGCAGC GGACGTGTCG 2821 TCCGCACCGC TGCCAAGTTA CGCCCACAGA AACCGCCACC GAGGCGCATC GTCAGGAACG 2881 CCAATACCAA CAACAACACC GTCACCGCAT CAAGCACCGC ACGCGCTAAC CCCACCCAAC 2941 GCACCAACGT GAATCTGAGC GTGCAAGCGT TCCCAAATCC AGGACAGAGC GCCTACAACG 3001 TGAATCCACC CGAATCAGCC TTCAAGGATT GCCCACCCCA AACCCTGCCA CCAGCCAGCA 3061 GTTTTCTGTC GAAAGCAGGC AGGCTGCTCG GCAGCGCTGC CATCCCTGCC GCATTGACCG 3121 CAGTTGGCGT CCCTGGCGTG GTCTCTGGAG TCGTCGGCAG CGCACTGGAT TCCGCGTTCT 3181 TGAGTCCCGC ACCGCCACCC ATGCAGAATG CACCATTGCC GACCGAGAAC GGCTGGTGGG 3241 GAGGCGTCCT ACCGTTGGCC TTGGGCACCG GCTTATGGAA ATGGTGGCAG TCCAAAAGGG 3301 GCTCAGGAGG GTTCATGCCA GCCGAACACA CACACATGGT TCGCAGGAAG GGACCAAGGA 3361 GGAGGATCGT CAGGAGGATC TATCGTGGCC CAAGACCCAG CAACCCAAGG GCAGAGCTGG 3421 ATCGCCCATT GCCACTCCAT ATGGGGGAAC ACAGGATCAT CAGGCGAGTC CAAAGCCCCT 3481 ATTCGTTGCC AGGCTTGTCC ACCATGATCC CTGGCCAGAT CATCAACCAG CGAGGCAACG 3541 TCGTCCCACC GCCAGGCCAG CAGGCCGAGC TCGCACCACT CCCACTCGCA CCACCTGCCC 3601 CACTCCCACC GCCACCTAAC GATTTCTTGG TTGGGCTGCA CGATCCACCT CCTGCCCCAA 3661 TGTTGCCACC GCCTCCACCG CCTCCACCAT TGCCACCTCC AAGCCCACCC CCATTGTTGA 3721 ACAACGACGA CCAGGACATC TTGAACATCG ACCAAGACGT CAACGTGCCA CCTCCACCGC 3781 CACCTGATTT CGGCCAATAC GATGACTATC CCCTGCCACC ATCGCCAGAA CCATTCGATC 3841 CACTGGTCGA TGAAGGCACC CCACTGCCCG ACCCACCCGA TGACTATCCC GATACCATCT 3901 CCGAAGTGTC ACTACCAACC CTGAGAGGCG ACAACGACGA AGAGCTATTC TTCTCGCCAG 3961 CAATCTCGCC ATATCCACCA CCGTCCGAGC ACTCTGGACG ACCTTACAGC CCAAACGGCA 4021 CCGTCACCAT CGAACCCGTC GAATACTCAC CACAAAGCGG ACCCAGCTAC AATCTCCGCG 4081 AGCGACCCCA GCAGGCCAGC TTCTTGGACG GGATCGTCGA CATGGCCTCC CGCTTGATCC 4141 CTGGACGCCG TCCAGCACCC GAAGTCCGAC CTCCGCCACC GGATAACAGC AGGAATGCCC 4201 TGCTCCAAGA TATCATCAGG AAGGCCCAGA GGATGAGGGA ACAACGCGAC AATCCAGTTC 4261 GCATCCAGTA TAGGCGCAGG CCACCCCCAA TCAGTAGCCC ACCTCCAACC ACATCAATCT 4321 ACGATTCACT GAACCGCCTG GTCGAGGGAC GCAGGAGCCA ACTGGGCTTG GACCAGGACG 4381 ACGATCCCGA CGTGCTGATG CAGGAGATGA GCGACTGGTA GCCTAGGACC CAGCTTTCTT 4441 GTACAAAGTG GTTCGATCTA GAATGGCTAG TGGATCCCCC GGGCTGCAGG AATTCGATAT 4501 CAAGCTTATC GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT 4561 TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC 4621 TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT 4681 TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA 4741 CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC 4801 TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC 4861 AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT 4921 TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT 4981 CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC 5041 TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC 5101 GCATCGATAC CGTCGGCCCA CTGCTCCCTA AACCTGAGCT AGCATTATCC CTAATACCTG 5161 CCACCCCACT CTTAATCAGT GGTGGAAGAA CGGTCTCAGA ACTGTTTGTT TCAATTGGCC 5221 ATTTAAGTTT AGTAGTAAAA GACTGGTTAA TGATAACAAT GCATCGTAAA ACCTTCAGAA 5281 GGAAAGGAGA ATGTTTTGTG GACCACTTTG GTTTTCTTTT TTGCGTGTGG CAGTTTTAAG 5341 TTATTAGTTT TTAAAATCAG TACTTTTTAA TGGAAACAAC TTGACCAAAA ATTTGTCACA 5401 GAATTTTGAG ACCCATTAAA AAAGTTAAAT GAGAAACCTG TGTGTTCCTT TGGTCAACAC 5461 CGAGACATTT AGGTGAAAGA CATCTAATTC TGGTTTTACG AATCTGGAAA CTTCTTGAAA 5521 ATGTAATTCT TGAGTTAACA CTTCTGGGTG GAGAATAGGG TTGTTTTCCC CCCACATAAT 5581 TGGAAGGGGA AGGAATATCA TTTAAAGCTA TGGGAGGGTT TCTTTGATTA CAACACTGGA 5641 GAGAAATGCA GCATGTTGCT GATTGCCTGT CACTAAAACA GGCCAAAAAC TGAGTCCTTN 5701 GGGTTGCATA GAAAGCTTCA TGTTGCTAAA CCAATGTTAA GTGAATCTTT GGAAACAAAA 5761 TGTTTCCAAA TTACTGGGAT GTGCATGTTG AAACGTGGGT TAATTAACTA GCCATGACCA 5821 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG 5881 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC 5941 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA 6001 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC 6061 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG 6121 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC 6181 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC 6241 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC 6301 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA 6361 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC 6421 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG 6481 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT 6541 TAATTAAATT TTTCAAAAGT AGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA 6601 TAATACGACT CACTATAGGA GGGCCATCAT GGCCAAGTTG ACCAGTGCTG TCCCAGTGCT 6661 CACAGCCAGG GATGTGGCTG GAGCTGTTGA GTTCTGGACT GACAGGTTGG GGTTCTCCAG 6721 AGATTTTGTG GAGGATGACT TTGCAGGTGT GGTCAGAGAT GATGTCACCC TGTTCATCTC 6781 AGCAGTCCAG GACCAGGTGG TGCCTGACAA CACCCTGGCT TGGGTGTGGG TGAGAGGACT 6841 GGATGAGCTG TATGCTGAGT GGAGTGAGGT GGTCTCCACC AACTTCAGGG ATGCCAGTGG 6901 CCCTGCCATG ACAGAGATTG GAGAGCAGCC CTGGGGGAGA GAGTTTGCCC TGAGAGACCC 6961 AGCAGGCAAC TGTGTGCACT TTGTGGCAGA GGAGCAGGAC TGAGGATAAG AATTGTAACA 7021 AAAAACCCCG CCCCGGCGGG GTTTTTTGTT AATTAA //