LOCUS pGwf 6361 bp ds-DNA circular SYN 17-Jun-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers misc_feature complement(<49..57) /note="SV40 minor late 19S RNA" promoter <49..>393 /note="SV40 promoter ( SELP)" enhancer 71..213 /note="SV40 enhancer elements" misc_feature 218..281 /note="SV40 21bp repeats" rep_origin 238..>373 /note="SV40 Ori" mRNA 287..293 /note="late-early transcription startpoints" mutation 307 /note="G->A kills SELP ATG" mRNA 327..333 /note="early-early transcription start points" CDS 418..1137 /note="EGFP" polyA_signal 1153..>1374 /note="SV40 Late PolyA" mutation 1444 /note="missing A (h Lsf sequencing)" promoter <1465..2643 /note="EF1a promoter and UTR" misc_difference 1465..>1656 /note="rhesus-derived" exon 1663..1695 /note="EF-1a exon 1" intron 1696..2635 /note="EF-1a intron A" exon 2635..2643 /note="EF-1a exon 2 leader" misc_recomb 2667..2691 /note="attB1" CDS 2697..2717 /note="Tev recognition (ENLYFQ//G)" CDS 2718..3782 /note="JCV VP1" misc_difference 2880..2882 /note="wt 55Leu" mRNA 3819..>4407 /note="WPRE" mutation 4408 /note="missing CTG" polyA_signal 4443..5112 /note="hEF1a polyA signal" polyA_site 4741 /note="site of polyA addition" CDS 5005 /note="This Sty1 doesn't cut (Gwf Asylum ReX Jan2010)" rep_origin 5113..5846 /note="MB1 Ori" promoter 5847..5933 /note="EM7 promoter" CDS 5934..6308 /note="Zeo" terminator 6309..6361 /note="terminator (rpmB/G)" BASE COUNT 1448 A 1697 C 1741 G 1472 T 1 OTHER ORIGIN ? 1 CCCTGCAGGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT GTGGAATGTG 61 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 121 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 181 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 241 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 301 ATTTATACAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 361 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTTGATTGGG ATCCACCGGT CGCCACCATG 421 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC 481 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC 541 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC 601 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG 661 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC 721 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG 781 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG 841 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC 901 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC 961 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC 1021 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG 1081 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC 1141 GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA 1201 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA 1261 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC 1321 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG 1381 ATAAGGATCC GGGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT 1441 TGCGGTGGAG AAGAGCATGC GTGAGGCTCC GGTGCCCGTC AGTGGGCAGA GCGCACATCG 1501 CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACCGGTGC CTAGAGAAGG 1561 TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCCTTT TCCCGAGGGT 1621 GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCTGTGAACG TTCTTTTTCG CAACGGGTTT 1681 GCCGCCAGAA CACAGGTAAG TGCCGTGTGT GGTTCCCGCG GGCCTGGCCT CTTTACGGGT 1741 TATGGCCCTT GCGTGCCTTG AATTACTTCC ACCTGGCTGC AGTACGTGAT TCTTGATCCC 1801 GAGCTTCGGG TTGGAAGTGG GTGGGAGAGT TCGAGGCCTT GCGCTTAAGG AGCCCCTTCG 1861 CCTCGTGCTT GAGTTGAGGC CTGGCCTGGG CGCTGGGGCC GCCGCGTGCG AATCTGGTGG 1921 CACCTTCGCG CCTGTCTCGC TGCTTTCGAT AAGTCTCTAG CCATTTAAAA TTTTTGATGA 1981 CCTGCTGCGA CGCTTTTTTT CTGGCAAGAT AGTCTTGTAA ATGCGGGCCA AGATCTGCAC 2041 ACTGGTATTT CGGTTTTTGG GGCCGCGGGC GGCGACGGGG CCCGTGCGTC CCAGCGCACA 2101 TGTTCGGCGA GGCGGGGCCT GCGAGCGCGG CCACCGAGAA TCGGACGGGG GTAGTCTCAA 2161 GCTGGCCGGC CTGCTCTGGT GCCTGGCCTC GCGCCGCCGT GTATCGCCCC GCCCTGGGCG 2221 GCAAGGCTGG CCCGGTCGGC ACCAGTTGCG TGAGCGGAAA GATGGCCGCT TCCCGGCCCT 2281 GCTGCAGGGA GCTCAAAATG GAGGACGCGG CGCTCGGGAG AGCGGGCGGG TGAGTCACCC 2341 ACACAAAGGA AAAGGGCCTT TCCGTCCTCA GCCGTCGCTT CATGTGACTC CACGGAGTAC 2401 CGGGCGCCGT CCAGGCACCT CGATTAGTTC TCGAGCTTTT GGAGTACGTC GTCTTTAGGT 2461 TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA GACTGAAGTT 2521 AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT 2581 TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG 2641 TGAGGAATTC TCTAGAGCTT GATCAAACAA GTTTGTACAA AAAAGCAGGC TCCGGAGAAA 2701 ACCTGTATTT TCAGGGCATG GCACCCACAA AGCGCAAGGG CGAACGCAAG GACCCAGTCC 2761 AGGTCCCCAA GTTGCTGATC AGGGGCGGCG TGGAGGTCCT GGAGGTCAAG ACCGGCGTGG 2821 ACTCCATCAC CGAGGTGGAG TGTTTCTTGA CACCCGAGAT GGGCGACCCC GACGAGCACT 2881 TGCGCGGGTT TTCGAAGAGC ATCAGCATCA GCGACACATT CGAGTCCGAC TCACCCAATA 2941 AGGACATGTT GCCATGCTAT TCCGTCGCCC GCATCCCACT GCCCAACCTC AACGAAGACT 3001 TGACATGCGG CAACATCCTG ATGTGGGAAG CCGTCACACT GAAGACCGAA GTCATCGGCG 3061 TCACCACCCT GATGAATGTG CACTCCAACG GCCAAGCGGC GCACGACAAC GGCGCCGGGA 3121 AGCCAGTCCA GGGGACAAGC TTCCATTTCT TCAGCGTCGG CGGCGAGGCA CTCGAGCTGC 3181 AAGGCGTGGT GTTCAACTAT CGCACCAAGT ATCCCGACGG CACCATATTC CCCAAGAACG 3241 CAACCGTCCA AAGTCAGGTC ATGAACACCG AGCACAAAGC ATATCTCGAT AAGAATAAGG 3301 CCTACCCCGT CGAGTGTTGG GTCCCCGATC CAACCAGGAA CGAGAACACC CGCTACTTCG 3361 GCACCCTGAC CGGCGGCGAG AACGTCCCAC CCGTGTTGCA CATCACAAAT ACCGCCACAA 3421 CCGTCCTGCT CGACGAGTTC GGCGTCGGCC CACTCTGCAA GGGAGACAAC CTCTACCTGA 3481 GCGCCGTCGA CGTCTGCGGA ATGTTCACCA ATCGAAGCGG CAGCCAGCAA TGGCGCGGGT 3541 TGTCCCGCTA CTTCAAAGTC CAGCTCCGCA AGCGCCGCGT GAAGAATCCC TATCCAATCA 3601 GTTTCCTGCT GACCGATTTG ATCAATCGCC GCACACCACG CGTCGACGGC CAGCCCATGT 3661 ACGGCATGGA CGCCCAAGTC GAAGAAGTGC GCGTATTCGA GGGGACCGAG GAGTTGCCCG 3721 GCGATCCCGA CATGATGCGC TACGTCGATA AATACGGCCA GCTCCAAACC AAGATGCTAT 3781 GACCCGGGCT GCAGGAATTC GATATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA 3841 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG 3901 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT 3961 TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG 4021 GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT 4081 GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG 4141 CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG 4201 TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC 4261 TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC 4321 GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC 4381 GGATCTCCCT TTGGGCCGCC TCCCCGCATC GATACCGTCG GCCCACTGCT CCCTAAACCT 4441 GAGCTAGCAT TATCCCTAAT ACCTGCCACC CCACTCTTAA TCAGTGGTGG AAGAACGGTC 4501 TCAGAACTGT TTGTTTCAAT TGGCCATTTA AGTTTAGTAG TAAAAGACTG GTTAATGATA 4561 ACAATGCATC GTAAAACCTT CAGAAGGAAA GGAGAATGTT TTGTGGACCA CTTTGGTTTT 4621 CTTTTTTGCG TGTGGCAGTT TTAAGTTATT AGTTTTTAAA ATCAGTACTT TTTAATGGAA 4681 ACAACTTGAC CAAAAATTTG TCACAGAATT TTGAGACCCA TTAAAAAAGT TAAATGAGAA 4741 ACCTGTGTGT TCCTTTGGTC AACACCGAGA CATTTAGGTG AAAGACATCT AATTCTGGTT 4801 TTACGAATCT GGAAACTTCT TGAAAATGTA ATTCTTGAGT TAACACTTCT GGGTGGAGAA 4861 TAGGGTTGTT TTCCCCCCAC ATAATTGGAA GGGGAAGGAA TATCATTTAA AGCTATGGGA 4921 GGGTTTCTTT GATTACAACA CTGGAGAGAA ATGCAGCATG TTGCTGATTG CCTGTCACTA 4981 AAACAGGCCA AAAACTGAGT CCTTNGGGTT GCATAGAAAG CTTCATGTTG CTAAACCAAT 5041 GTTAAGTGAA TCTTTGGAAA CAAAATGTTT CCAAATTACT GGGATGTGCA TGTTGAAACG 5101 TGGGTTAATT AACTAGCCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG 5161 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC 5221 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG 5281 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT 5341 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC 5401 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC 5461 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT 5521 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT 5581 GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC 5641 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT 5701 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA 5761 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT 5821 TGCTGGCCTT TTGCTCACAT GTTCTTAATT AAATTTTTCA AAAGTAGTTG ACAATTAATC 5881 ATCGGCATAG TATATCGGCA TAGTATAATA CGACTCACTA TAGGAGGGCC ATCATGGCCA 5941 AGTTGACCAG TGCTGTCCCA GTGCTCACAG CCAGGGATGT GGCTGGAGCT GTTGAGTTCT 6001 GGACTGACAG GTTGGGGTTC TCCAGAGATT TTGTGGAGGA TGACTTTGCA GGTGTGGTCA 6061 GAGATGATGT CACCCTGTTC ATCTCAGCAG TCCAGGACCA GGTGGTGCCT GACAACACCC 6121 TGGCTTGGGT GTGGGTGAGA GGACTGGATG AGCTGTATGC TGAGTGGAGT GAGGTGGTCT 6181 CCACCAACTT CAGGGATGCC AGTGGCCCTG CCATGACAGA GATTGGAGAG CAGCCCTGGG 6241 GGAGAGAGTT TGCCCTGAGA GACCCAGCAG GCAACTGTGT GCACTTTGTG GCAGAGGAGC 6301 AGGACTGAGG ATAAGAATTG TAACAAAAAA CCCCGCCCCG GCGGGGTTTT TTGTTAATTA //