LOCUS pGwf 6418 bp DNA circular SYN 17-Jun-2003 DEFINITION . FEATURES Location/Qualifiers misc_feature complement(<49..57) /note="SV40 minor late 19S RNA" promoter <49..>393 /note="SV40 promoter ( SELP)" enhancer 71..213 /note="SV40 enhancer elements" misc_feature 218..281 /note="SV40 21bp repeats" rep_origin 238..>373 /note="SV40 Ori" mRNA 287..293 /note="late-early transcription startpoints" mutation 307 /note="G->A kills SELP ATG" mRNA 327..333 /note="early-early transcription start points" CDS 418..1137 /note="EGFP" polyA_signal 1153..>1374 /note="SV40 Late PolyA" mutation 1444 /note="missing A (h Lsf sequencing)" promoter <1465..2643 /note="EF1a promoter and UTR" misc_difference 1465..>1656 /note="rhesus-derived" exon 1663..1695 /note="EF-1a exon 1" intron 1696..2635 /note="EF-1a intron A" exon 2635..2643 /note="EF-1a exon 2 leader" misc_recomb 2667..2691 /note="attB1" CDS 2697..2717 /note="Tev recognition (ENLYFQ//G)" CDS 2718..3782 /note="JCV-720B (2A) VP1" misc_recomb 3496..3561 /note="JCV 270 region" misc_recomb 3516..3518 /note="S267 (F, with Q271H in PML)" misc_recomb 3522..3524 /note="S269 (F is common in PML)" misc_recomb 3528..3530 /note="Q271 (H, occurs with S267F in PML)" misc_recomb 3790..3814 /note="attB2" mRNA 3876..>4464 /note="WPRE" mutation 4465 /note="missing CTG" polyA_signal 4500..5169 /note="hEF1a polyA signal" polyA_site 4798 /note="site of polyA addition" CDS 5062 /note="This Sty1 doesn't cut (Gwf Asylum ReX Jan2010)" rep_origin 5170..5903 /note="MB1 Ori" promoter 5904..5990 /note="EM7 promoter" CDS 5991..6365 /note="Zeo" terminator 6366..6418 /note="terminator (rpmB/G)" BASE COUNT 1461 A 1715 C 1756 G 1485 T 1 OTHER ORIGIN 1 CCCTGCAGGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT GTGGAATGTG 61 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 121 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 181 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 241 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 301 ATTTATACAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 361 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTTGATTGGG ATCCACCGGT CGCCACCATG 421 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC 481 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC 541 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC 601 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG 661 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC 721 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG 781 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG 841 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC 901 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC 961 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC 1021 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG 1081 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC 1141 GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA 1201 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA 1261 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC 1321 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG 1381 ATAAGGATCC GGGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT 1441 TGCGGTGGAG AAGAGCATGC GTGAGGCTCC GGTGCCCGTC AGTGGGCAGA GCGCACATCG 1501 CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACCGGTGC CTAGAGAAGG 1561 TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCCTTT TCCCGAGGGT 1621 GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCTGTGAACG TTCTTTTTCG CAACGGGTTT 1681 GCCGCCAGAA CACAGGTAAG TGCCGTGTGT GGTTCCCGCG GGCCTGGCCT CTTTACGGGT 1741 TATGGCCCTT GCGTGCCTTG AATTACTTCC ACCTGGCTGC AGTACGTGAT TCTTGATCCC 1801 GAGCTTCGGG TTGGAAGTGG GTGGGAGAGT TCGAGGCCTT GCGCTTAAGG AGCCCCTTCG 1861 CCTCGTGCTT GAGTTGAGGC CTGGCCTGGG CGCTGGGGCC GCCGCGTGCG AATCTGGTGG 1921 CACCTTCGCG CCTGTCTCGC TGCTTTCGAT AAGTCTCTAG CCATTTAAAA TTTTTGATGA 1981 CCTGCTGCGA CGCTTTTTTT CTGGCAAGAT AGTCTTGTAA ATGCGGGCCA AGATCTGCAC 2041 ACTGGTATTT CGGTTTTTGG GGCCGCGGGC GGCGACGGGG CCCGTGCGTC CCAGCGCACA 2101 TGTTCGGCGA GGCGGGGCCT GCGAGCGCGG CCACCGAGAA TCGGACGGGG GTAGTCTCAA 2161 GCTGGCCGGC CTGCTCTGGT GCCTGGCCTC GCGCCGCCGT GTATCGCCCC GCCCTGGGCG 2221 GCAAGGCTGG CCCGGTCGGC ACCAGTTGCG TGAGCGGAAA GATGGCCGCT TCCCGGCCCT 2281 GCTGCAGGGA GCTCAAAATG GAGGACGCGG CGCTCGGGAG AGCGGGCGGG TGAGTCACCC 2341 ACACAAAGGA AAAGGGCCTT TCCGTCCTCA GCCGTCGCTT CATGTGACTC CACGGAGTAC 2401 CGGGCGCCGT CCAGGCACCT CGATTAGTTC TCGAGCTTTT GGAGTACGTC GTCTTTAGGT 2461 TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA GACTGAAGTT 2521 AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT 2581 TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG 2641 TGAGGAATTC TCTAGAGCTT GATCAAACAA GTTTGTACAA AAAAGCAGGC TCCGGAGAAA 2701 ACCTGTATTT TCAGGGCATG GCACCCACAA AGCGCAAGGG CGAACGCAAG GACCCAGTCC 2761 AGGTCCCCAA GTTGCTGATC AGGGGCGGCG TGGAGGTCCT GGAGGTCAAG ACCGGCGTGG 2821 ACTCCATCAC CGAGGTGGAG TGTTTCTTGA CACCCGAGAT GGGCGACCCC GACGAGCACC 2881 TGCGCGGGTT CAGCAAGAGC ATCAGCATCA GCGACACATT CGAGTCCGAC TCACCCAATA 2941 AGGACATGTT GCCATGCTAT TCCGTCGCCC GCATCCCACT GCCCAACCTC AACGAAGACT 3001 TGACATGCGG CAACATCCTG ATGTGGGAAG CCGTCACACT GAAGACCGAA GTCATCGGCG 3061 TCACCACCCT GATGAATGTG CACTCCAACG GCCAAGCGAC GCACGACAAC GGCGCCGGGA 3121 AGCCAGTCCA GGGGACAAGC TTCCATTTCT TCAGCGTCGG CGGCGAGGCA CTCGAGCTGC 3181 AAGGCGTGGT GTTCAACTAT CGCACCAAGT ATCCCGACGG CACCATATTC CCCAAGAACG 3241 CAACCGTCCA AAGTCAGGTC ATGAACACCG AGCACAAAGC ATATCTCGAT AAGAATAAGG 3301 CCTACCCCGT CGAGTGTTGG GTCCCCGATC CAACCAGGAA CGAGAACACC CGCTACTTCG 3361 GCACCCTGAC CGGCGGCGAG AACGTCCCAC CCGTGTTGCA CATCACAAAT ACCGCCACAA 3421 CCGTCCTGCT CGACGAGTTC GGCGTCGGCC CACTCTGCAA GGGAGACAAC CTCTACCTGA 3481 GCGCCGTCGA CGTCTGCGGA ATGTTCACCA ATCGAAGCGG CAGCCAGCAA TGGCGCGGGT 3541 TGTCCCGCTA CTTCAAAGTC CAGCTCCGCA AGCGCCGCGT GAAGAATCCC TATCCAATCA 3601 GTTTCCTGCT GACCGATTTG ATCAATCGCC GCACACCACG CGTCGACGGC CAGCCCATGT 3661 ACGGCATGGA CGCCCAAGTC GAAGAAGTGC GCGTATTCGA GGGGACCGAG GAGTTGCCCG 3721 GCGATCCCGA CATGATGCGC TACGTCGATC GCTACGGCCA GCTCCAAACC AAGATGCTAT 3781 GAGCCTAGGA CCCAGCTTTC TTGTACAAAG TGGTTCGATC TAGAATGGCT AGTGGATCCC 3841 CCGGGCTGCA GGAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT 3901 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 3961 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT 4021 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG 4081 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC 4141 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG 4201 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT 4261 TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC 4321 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG 4381 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA 4441 TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGTCGGCC CACTGCTCCC TAAACCTGAG 4501 CTAGCATTAT CCCTAATACC TGCCACCCCA CTCTTAATCA GTGGTGGAAG AACGGTCTCA 4561 GAACTGTTTG TTTCAATTGG CCATTTAAGT TTAGTAGTAA AAGACTGGTT AATGATAACA 4621 ATGCATCGTA AAACCTTCAG AAGGAAAGGA GAATGTTTTG TGGACCACTT TGGTTTTCTT 4681 TTTTGCGTGT GGCAGTTTTA AGTTATTAGT TTTTAAAATC AGTACTTTTT AATGGAAACA 4741 ACTTGACCAA AAATTTGTCA CAGAATTTTG AGACCCATTA AAAAAGTTAA ATGAGAAACC 4801 TGTGTGTTCC TTTGGTCAAC ACCGAGACAT TTAGGTGAAA GACATCTAAT TCTGGTTTTA 4861 CGAATCTGGA AACTTCTTGA AAATGTAATT CTTGAGTTAA CACTTCTGGG TGGAGAATAG 4921 GGTTGTTTTC CCCCCACATA ATTGGAAGGG GAAGGAATAT CATTTAAAGC TATGGGAGGG 4981 TTTCTTTGAT TACAACACTG GAGAGAAATG CAGCATGTTG CTGATTGCCT GTCACTAAAA 5041 CAGGCCAAAA ACTGAGTCCT TNGGGTTGCA TAGAAAGCTT CATGTTGCTA AACCAATGTT 5101 AAGTGAATCT TTGGAAACAA AATGTTTCCA AATTACTGGG ATGTGCATGT TGAAACGTGG 5161 GTTAATTAAC TAGCCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA 5221 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC 5281 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA 5341 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT 5401 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC 5461 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG 5521 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG 5581 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG 5641 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC 5701 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT 5761 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG 5821 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC 5881 TGGCCTTTTG CTCACATGTT CTTAATTAAA TTTTTCAAAA GTAGTTGACA ATTAATCATC 5941 GGCATAGTAT ATCGGCATAG TATAATACGA CTCACTATAG GAGGGCCATC ATGGCCAAGT 6001 TGACCAGTGC TGTCCCAGTG CTCACAGCCA GGGATGTGGC TGGAGCTGTT GAGTTCTGGA 6061 CTGACAGGTT GGGGTTCTCC AGAGATTTTG TGGAGGATGA CTTTGCAGGT GTGGTCAGAG 6121 ATGATGTCAC CCTGTTCATC TCAGCAGTCC AGGACCAGGT GGTGCCTGAC AACACCCTGG 6181 CTTGGGTGTG GGTGAGAGGA CTGGATGAGC TGTATGCTGA GTGGAGTGAG GTGGTCTCCA 6241 CCAACTTCAG GGATGCCAGT GGCCCTGCCA TGACAGAGAT TGGAGAGCAG CCCTGGGGGA 6301 GAGAGTTTGC CCTGAGAGAC CCAGCAGGCA ACTGTGTGCA CTTTGTGGCA GAGGAGCAGG 6361 ACTGAGGATA AGAATTGTAA CAAAAAACCC CGCCCCGGCG GGGTTTTTTG TTAATTAA //