LOCUS pu_____w 5886 bp ds-DNA circular SYN 26-Apr-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Plasmid from Blueheron biotechnology. Used for cloning codon optimized genes into it. FEATURES Location/Qualifiers promoter 113..310 /note="EF-1alpha promoter core" promoter 113..1291 /note="EF1a promoter and UTR" exon 311..343 /note="EF-1a exon 1" intron 344..1283 /note="EF-1a intron A" exon 1283..1291 /note="EF-1a exon 2 leader" mutation 1292 /note="extra G discovered by sequencing" CDS 1304..2668 /note="Muller HPV11 L2" mutation 1823..1825 /note="this Ser is Asn in AF512010 and HPV6" mRNA 2703..>3291 /note="WPRE" mutation 3292 /note="CTG missing" polyA_signal 3339..>3564 /note="SV40 late polyA" rep_origin 3565..4319 /note="MB1 ori" enhancer 4347..4489 /note="SV40 enhancer elements" repeat_region 4494..4557 /note="SV40 21 bp repeats" promoter 4494..4668 /note="SV40 early promoter" rep_origin 4517..4668 /note="SV40 ori" mRNA 4566..4615 /note="SV40 transcription start points" promoter 4703..4758 /note="EM7" CDS 4778..5572 /note="KanR" polyA_signal 5620..5886 /note="HSV TK pA signal" polyA_site 5678..5696 /note="HSV TK pA site" BASE COUNT 1172 A 1755 C 1657 G 1300 T 0 OTHER ORIGIN ? 1 CCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTGCAAAGA TGGATAAAGT TTTAAACAGA 61 GAGGAATCTT TGCAGCTAAT GGACCTTGTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 121 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 181 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 241 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 301 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 361 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 421 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 481 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 541 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 601 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 661 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 721 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 781 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 841 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 901 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 961 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1021 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1081 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1141 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1201 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1261 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGATGAAGC CCAGAGCCCG 1321 GCGGAGAAAG CGGGCCAGCG CCACCCAGCT GTACCAGACC TGCAAGGCCA CCGGCACCTG 1381 CCCCCCTGAC GTGATCCCCA AGGTGGAGCA CACCACCATC GCCGACCAGA TCCTGAAGTG 1441 GGGCAGCCTG GGCGTGTTCT TCGGCGGCCT GGGCATCGGC ACCGGCGCCG GCAGCGGCGG 1501 CCGGGCCGGC TACATCCCCC TGGGCAGCAG CCCTAAGCCC GCCATCACCG GGGGCCCTGC 1561 CGCCAGACCC CCCGTGCTGG TGGAGCCTGT GGCCCCCAGC GACCCCAGCA TCGTGAGTCT 1621 GATCGAGGAG AGCGCCATCA TCAACGCCGG CGCCCCTGAG GTGGTGCCTC CTACCCAGGG 1681 CGGCTTCACC ATCACCAGCA GCGAGAGCAC CACCCCTGCC ATCCTGGACG TGAGCGTGAC 1741 CAACCACACC ACCACCAGCG TGTTCCAGAA CCCCCTGTTC ACCGAGCCTA GCGTGATCCA 1801 GCCCCAGCCT CCCGTGGAGG CCAGCGGCCA CATCCTGATC AGCGCCCCTA CCATCACCAG 1861 CCAGCACGTG GAGGACATCC CCCTGGACAC CTTCGTGGTG AGCAGCAGCG ACAGCGGCCC 1921 CACCAGCAGC ACCCCTCTGC CCCGGGCCTT CCCCAGACCT CGGGTGGGCC TGTACAGCAG 1981 AGCCCTGCAG CAGGTGCAGG TGACCGACCC CGCCTTCCTG AGCACCCCCC AGCGGCTGGT 2041 GACCTACGAC AACCCTGTGT ACGAGGGCGA GGACGTGAGC CTGCAGTTCA CCCACGAGAG 2101 CATCCACAAC GCCCCCGACG AGGCCTTCAT GGATATCATC AGACTGCACC GGCCCGCCAT 2161 CACCAGCAGA CGGGGCCTGG TGCGCTTCAG CAGAATCGGC CAGCGGGGCA GCATGTACAC 2221 CCGGAGCGGC CAGCACATCG GCGCCAGAAT TCACTACTTC CAGGACATCA GCCCTGTGAC 2281 CCAGGCCGCC GAGGAGATCG AGCTGCACCC CCTGGTGGCC GCCGAGAACG ACACCTTCGA 2341 CATCTACGCC GAGCCCTTCG ACCCTATCCC CGACCCCGTG CAGCACAGCG TGACCCAGAG 2401 CTACCTGACC AGCACCCCTA ACACCCTGAG CCAGAGCTGG GGCAACACCA CCGTGCCCCT 2461 GAGCATCCCC AGCGACTGGT TCGTGCAGAG CGGCCCCGAC ATCACCTTCC CTACCGCTAG 2521 CATGGGCACC CCCTTCAGCC CCGTGACCCC TGCCCTGCCC ACCGGCCCCG TGTTCATCAC 2581 CGGCAGCGAC TTCTACCTGC ACCCTACCTG GTACTTCGCC CGGCGGAGAC GGAAGCGGAT 2641 CCCCCTGTTC TTCACCGACG TGGCCGCCTA AGCTTATCGA TACCGTCATC AAGCTTATCG 2701 ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG 2761 CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC 2821 GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT 2881 TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA 2941 CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC 3001 CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC 3061 TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC 3121 TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC 3181 TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC 3241 TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CATCGATACC 3301 GTCGGCCGCT TCCCTTTAGT GAGGGTTAAT GCTTCGAGCA GACATGATAA GATACATTGA 3361 TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG 3421 TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA 3481 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA 3541 AAACCTCTAC AAATGTGGTA TGGAATTCTT AATTAACTAG CCATGACCAA AATCCCTTAA 3601 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA 3661 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG 3721 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC 3781 AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG 3841 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC 3901 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG 3961 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC 4021 ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA 4081 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT 4141 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG 4201 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG 4261 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT AATTAAGCTG 4321 TACACTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG 4381 AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC 4441 CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC 4501 CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGA 4561 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA 4621 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG 4681 TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC 4741 GGCATAGTAT AATACGACTC ACTATAGGAG GGCCACCATG ATTGAACAAG ATGGATTGCA 4801 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC 4861 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT 4921 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA GACGAGGCAG CGCGGCTATC 4981 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG 5041 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC 5101 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC 5161 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT 5221 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC 5281 CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC GAGGATCTCG TCGTGACACA 5341 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA 5401 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT 5461 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC 5521 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT 5581 CTGGGGTTCG AAATGACCGA CCAAGCGAAT TCGCTAGAGG GAGGCTAACT GAAACACGGA 5641 AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC 5701 ACGGTGTTGG GTCGTTTGTT CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG 5761 ATACCCCACC GAGACCCCAT TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC 5821 ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG 5881 CCATAG //