LOCUS PWP.GB MAY 6493 BP DS-DNA CIRCULAR SYN 17-MAY-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers misc_feature complement(>49..57) /note="SV40 minor late 19S RNA" promoter <49..>393 /note="SV40 promoter (∆SELP)" enhancer 71..213 /note="SV40 enhancer elements" misc_feature 218..281 /note="SV40 21bp repeats" rep_origin 238..>373 /note="SV40 Ori" mRNA 287..293 /note="late-early transcription startpoints" mutation 307..307 /note="G->A kills SELP ATG" mRNA 327..333 /note="early-early transcription start points" CDS 418..1137 /note="EGFP" polyA_signal 1153..>1374 /note="SV40 Late PolyA" promoter <1465..2643 /note="EF1a promoter and UTR" misc_difference 1465..>1656 /note="rhesus-derived" exon 1663..1695 /note="EF-1a exon 1" intron 1696..2635 /note="EF-1a intron A" exon 2635..2643 /note="EF-1a exon 2 leader" insertion_seq 2667..>2691 /note="attB1" CDS 2704..3858 /note="MPyV VP1 (codmod)" misc_recomb 3866..>3890 /note="attB2" mRNA 3952..>4540 /note="WPRE" polyA_signal 4576..5244 /note="hEF1a polyA signal" polyA_site 4874..4874 /note="site of polyA addition" rep_origin 5245..5978 /note="MB1 Ori" promoter 5979..6065 /note="EM7 promoter" CDS 6066..6440 /note="Zeocin resistance" terminator 6441..6493 /note="terminator (rpmB/G)" BASE COUNT 1502 A 1737 C 1770 G 1484 T 0 OTHER ORIGIN - 1 CCCTGCAGGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT GTGGAATGTG 61 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 121 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 181 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 241 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 301 ATTTATACAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 361 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTTGATTGGG ATCCACCGGT CGCCACCATG 421 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC 481 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC 541 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC 601 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG 661 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC 721 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG 781 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG 841 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC 901 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC 961 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC 1021 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG 1081 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC 1141 GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA 1201 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA 1261 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC 1321 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG 1381 ATAAGGATCC GGGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT 1441 TGCGGTGGAG AAGAGCATGC GTGAGGCTCC GGTGCCCGTC AGTGGGCAGA GCGCACATCG 1501 CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACCGGTGC CTAGAGAAGG 1561 TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCCTTT TCCCGAGGGT 1621 GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCTGTGAACG TTCTTTTTCG CAACGGGTTT 1681 GCCGCCAGAA CACAGGTAAG TGCCGTGTGT GGTTCCCGCG GGCCTGGCCT CTTTACGGGT 1741 TATGGCCCTT GCGTGCCTTG AATTACTTCC ACCTGGCTGC AGTACGTGAT TCTTGATCCC 1801 GAGCTTCGGG TTGGAAGTGG GTGGGAGAGT TCGAGGCCTT GCGCTTAAGG AGCCCCTTCG 1861 CCTCGTGCTT GAGTTGAGGC CTGGCCTGGG CGCTGGGGCC GCCGCGTGCG AATCTGGTGG 1921 CACCTTCGCG CCTGTCTCGC TGCTTTCGAT AAGTCTCTAG CCATTTAAAA TTTTTGATGA 1981 CCTGCTGCGA CGCTTTTTTT CTGGCAAGAT AGTCTTGTAA ATGCGGGCCA AGATCTGCAC 2041 ACTGGTATTT CGGTTTTTGG GGCCGCGGGC GGCGACGGGG CCCGTGCGTC CCAGCGCACA 2101 TGTTCGGCGA GGCGGGGCCT GCGAGCGCGG CCACCGAGAA TCGGACGGGG GTAGTCTCAA 2161 GCTGGCCGGC CTGCTCTGGT GCCTGGCCTC GCGCCGCCGT GTATCGCCCC GCCCTGGGCG 2221 GCAAGGCTGG CCCGGTCGGC ACCAGTTGCG TGAGCGGAAA GATGGCCGCT TCCCGGCCCT 2281 GCTGCAGGGA GCTCAAAATG GAGGACGCGG CGCTCGGGAG AGCGGGCGGG TGAGTCACCC 2341 ACACAAAGGA AAAGGGCCTT TCCGTCCTCA GCCGTCGCTT CATGTGACTC CACGGAGTAC 2401 CGGGCGCCGT CCAGGCACCT CGATTAGTTC TCGAGCTTTT GGAGTACGTC GTCTTTAGGT 2461 TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA GACTGAAGTT 2521 AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT 2581 TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG 2641 TGAGGAATTC TCTAGAGCTT GATCAAACAA GTTTGTACAA AAAAGCAGGC TTCCGGAACC 2701 ACCATGGCAC CAAAGCGCAA GTCAGGGGTC AGCAAATGTG AAACCAAGTG CACCAAGGCA 2761 TGCCCCCGCC CTGCACCCGT CCCAAAGCTC TTGATCAAGG GCGGCATGGA AGTCCTCGAT 2821 CTGGTCACCG GCCCCGATTC CGTCACCGAG ATCGAGGCAT TCCTGAATCC ACGCATGGGC 2881 CAACCCCCAA CACCCGAGTC ACTGACCGAA GGCGGCCAGT ATTACGGCTG GAGCCGCGGC 2941 ATCAACCTCG CCACCAGCGA CACCGAAGAC AGTCCCGAGA ACAACACCTT GCCAACCTGG 3001 TCCATGGCCA AACTGCAACT GCCAATGCTG AACGAAGATC TGACATGCGA TACATTGCAA 3061 ATGTGGGAAG CCGTCAGCGT CAAGACAGAA GTCGTCGGGA GCGGGAGCCT GCTCGATGTC 3121 CACGGCTTCA ACAAGCCAAC CGACACCGTG AACACCAAGG GCATCAGTAC CCCGGTTGAG 3181 GGGTCACAGT ACCACGTCTT CGCCGTCGGG GGCGAGCCCC TGGATCTGCA AGGCTTGGTC 3241 ACCGACGCAC GCACCAAGTT CAAAGAGGAG GGCGTGGTCA CCATCAAGAC CATTACCAAG 3301 AAGGACATGG TCAACAAGGA TCAGGTGCTC AACCCCATCT CAAAAGCAAA GCTCGACAAG 3361 GATGGCATGT ACCCCGTCGA GATTTGGCAC CCCGACCCCG CCAAGAACGA AAACACCCGC 3421 TACTTCGGGA ACTATACCGG CGGGACCACC ACCCCACCGG TGCTCCAATT CACCAATACA 3481 CTCACCACCG TCCTGCTGGA CGAGAACGGC GTCGGCCCAC TGTGCAAGGG CGAAGGGTTG 3541 TATCTGAGTT GCGTGGACAT CATGGGGTGG CGCGTCACCC GCAACTACGA CGCACACCAT 3601 TGGCGCGGCC TGCCACGCTA CTTCAAGATT ACACTCCGCA AGCGCTGGGT CAAGAACCCA 3661 TACCCAATGG CAAGTCTGAT CTCGAGTCTG TTCAACAATA TGTTGCCACA AGTCCAGGGG 3721 CAGCCAATGG AGGGCGAAAA CACACAAGTG GAAGAAGTCC GCGTCTACGA CGGCACCGAG 3781 CCCGTGCCCG GCGATCCCGA CATGACCAGA TACGTCGATA GGTTCGGCAA GACCAAAACC 3841 GTGTTCCCAG GAAACTGAGC CTAGGACCCA GCTTTCTTGT ACAAAGTGGT TCGATCTAGA 3901 ATGGCTAGTG GATCCCCCGG GCTGCAGGAA TTCGATATCA AGCTTATCGA TAATCAACCT 3961 CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG 4021 CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC 4081 ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT 4141 GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC 4201 ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG 4261 GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT 4321 GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT 4381 GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG 4441 GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC 4501 CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGGCCCACT 4561 GCTCCCTAAA CCTGAGCTAG CATTATCCCT AATACCTGCC ACCCCACTCT TAATCAGTGG 4621 TGGAAGAACG GTCTCAGAAC TGTTTGTTTC AATTGGCCAT TTAAGTTTAG TAGTAAAAGA 4681 CTGGTTAATG ATAACAATGC ATCGTAAAAC CTTCAGAAGG AAAGGAGAAT GTTTTGTGGA 4741 CCACTTTGGT TTTCTTTTTT GCGTGTGGCA GTTTTAAGTT ATTAGTTTTT AAAATCAGTA 4801 CTTTTTAATG GAAACAACTT GACCAAAAAT TTGTCACAGA ATTTTGAGAC CCATTAAAAA 4861 AGTTAAATGA GAAACCTGTG TGTTCCTTTG GTCAACACCG AGACATTTAG GTGAAAGACA 4921 TCTAATTCTG GTTTTACGAA TCTGGAAACT TCTTGAAAAT GTAATTCTTG AGTTAACACT 4981 TCTGGGTGGA GAATAGGGTT GTTTTCCCCC CACATAATTG GAAGGGGAAG GAATATCATT 5041 TAAAGCTATG GGAGGGTTTC TTTGATTACA ACACTGGAGA GAAATGCAGC ATGTTGCTGA 5101 TTGCCTGTCA CTAAAACAGG CCAAAAACTG AGTCCTTGGG TTGCATAGAA AGCTTCATGT 5161 TGCTAAACCA ATGTTAAGTG AATCTTTGGA AACAAAATGT TTCCAAATTA CTGGGATGTG 5221 CATGTTGAAA CGTGGGTTAA TTAACTAGCC ATGACCAAAA TCCCTTAACG TGAGTTTTCG 5281 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT 5341 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG 5401 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA 5461 CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA 5521 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG 5581 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC 5641 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA 5701 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG 5761 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC 5821 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG 5881 TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG 5941 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTAA TTAAATTTTT CAAAAGTAGT 6001 TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA TACGACTCAC TATAGGAGGG 6061 CCATCATGGC CAAGTTGACC AGTGCTGTCC CAGTGCTCAC AGCCAGGGAT GTGGCTGGAG 6121 CTGTTGAGTT CTGGACTGAC AGGTTGGGGT TCTCCAGAGA TTTTGTGGAG GATGACTTTG 6181 CAGGTGTGGT CAGAGATGAT GTCACCCTGT TCATCTCAGC AGTCCAGGAC CAGGTGGTGC 6241 CTGACAACAC CCTGGCTTGG GTGTGGGTGA GAGGACTGGA TGAGCTGTAT GCTGAGTGGA 6301 GTGAGGTGGT CTCCACCAAC TTCAGGGATG CCAGTGGCCC TGCCATGACA GAGATTGGAG 6361 AGCAGCCCTG GGGGAGAGAG TTTGCCCTGA GAGACCCAGC AGGCAACTGT GTGCACTTTG 6421 TGGCAGAGGA GCAGGACTGA GGATAAGAAT TGTAACAAAA AACCCCGCCC CGGCGGGGTT 6481 TTTTGTTAAT TAA //