LOCUS PUL2T 7483 BP DS-DNA CIRCULAR SYN 26-MAR-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Plasmid from Blueheron biotechnology. Used for cloning codon optimized genes into it. FEATURES Location/Qualifiers promoter 113..310 /note="EF-1alpha promoter core" promoter 113..1291 /note="EF1a promoter and UTR" exon 311..343 /note="EF-1a exon 1" intron 344..1283 /note="EF-1a intron A" exon 1283..1291 /note="EF-1a exon 2 leader" CDS 1396..>2814 /note="HPV16 L2 (codmod)" CDS 2815..>2841 /note="linker" CDS 2842..4272 /note="tdTomato (Roger Tsien)" mat_peptide 2856..3519 /note="dsRed repeat A" mat_peptide 3589..4245 /note="dsRed repeat B" mRNA 4300..>4888 /note="WPRE" polyA_signal 4936..>5161 /note="SV40 late polyA" rep_origin 5162..5916 /note="MB1 ori" repeat_region 6091..6154 /note="SV40 21 bp repeats" promoter 6091..6265 /note="SV40 early promoter" rep_origin 6114..6265 /note="SV40 ori" mRNA 6163..6212 /note="SV40 transcription start points" promoter 6300..6355 /note="EM7" CDS 6375..7169 /note="Kan/Neo resistance" polyA_signal 7217..7483 /note="HSV TK pA signal" polyA_site 7275..7293 /note="HSV TK pA site" BASE COUNT 1576 A 2319 C 2088 G 1500 T 0 OTHER ORIGIN - 1 CCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTGCAAAGA TGGATAAAGT TTTAAACAGA 61 GAGGAATCTT TGCAGCTAAT GGACCTTGTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 121 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 181 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 241 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 301 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 361 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 421 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 481 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 541 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 601 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 661 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 721 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 781 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 841 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 901 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 961 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1021 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1081 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1141 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1201 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1261 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA 1321 GATCCATCCT GTACGGAAGT GTTACTTCTG CTCTAAAAGC TGCGGAATTG TACCCGCGGC 1381 CGCTCTAGAG CCACCATGAG GCACAAGAGG AGCGCCAAGA GGACCAAGAG GGCCAGCGCC 1441 ACCCAGCTGT ACAAGACCTG CAAGCAGGCC GGCACCTGCC CCCCCGACAT CATCCCCAAG 1501 GTGGAGGGCA AGACCATCGC CGACCAGATC CTGCAGTACG GCAGCATGGG CGTGTTCTTC 1561 GGCGGCCTGG GCATCGGCAC CGGCAGCGGC ACCGGCGGCA GGACCGGCTA CATCCCCCTG 1621 GGCACCAGGC CCCCCACCGC CACCGACACC CTGGCCCCCG TGAGGCCCCC CCTGACCGTG 1681 GACCCCGTGG GCCCCAGCGA CCCCAGCATC GTGAGCCTGG TGGAGGAGAC CAGCTTCATC 1741 GACGCCGGCG CCCCCACCAG CGTGCCCAGC ATCCCCCCCG ACGTGAGCGG CTTCAGCATC 1801 ACCACCAGCA CCGACACCAC CCCCGCCATC CTGGACATCA ACAACACCGT GACCACCGTG 1861 ACCACCCACA ACAACCCCAC CTTCACCGAC CCCAGCGTGC TGCAGCCCCC CACCCCCGCC 1921 GAGACCGGCG GCCACTTCAC CCTGAGCAGC AGCACCATCA GCACCCACAA CTACGAGGAG 1981 ATCCCCATGG ACACCTTCAT CGTGAGCACC AACCCCAACA CCGTGACCAG CAGCACCCCC 2041 ATCCCCGGCA GCAGGCCCGT GGCCAGGCTG GGCCTGTACA GCAGGACCAC CCAGCAGGTG 2101 AAGGTGGTGG ACCCCGCCTT CGTGACCACC CCCACCAAGC TGATCACCTA CGACAACCCC 2161 GCCTACGAGG GCATCGACGT GGACAACACC CTGTACTTCA GCAGCAACGA CAACAGCATC 2221 AACATCGCCC CCGACCCCGA CTTCCTGGAC ATCGTGGCCC TGCACAGGCC CGCCCTGACC 2281 AGCAGGAGGA CCGGCATCAG GTACAGCAGG ATCGGCAACA AGCAGACCCT GAGGACCAGG 2341 AGCGGCAAGA GCATCGGCGC CAAGGTGCAC TACTACTACG ACCTGAGCAC CATCGACCCC 2401 GCCGAGGAGA TCGAGCTGCA GACCATCACC CCCAGCACCT ACACCACCAC CAGCCACGCC 2461 GCCAGCCCCA CCAGCATCAA CAACGGCCTG TACGACATCT ACGCCGACGA CTTCATCACC 2521 GACACCAGCA CCACCCCCGT GCCCAGCGTG CCCAGCACCA GCCTGAGCGG CTACATCCCC 2581 GCCAACACCA CCATCCCCTT CGGTGGCGCC TACAACATCC CCCTGGTGAG CGGCCCCGAC 2641 ATCCCCATCA ACATCACCGA CCAGGCCCCC AGCCTGATCC CCATCGTGCC CGGCAGCCCC 2701 CAGTACACCA TCATCGCCGA CGCCGGCGAC TTCTACCTGC ACCCCAGCTA CTACATGCTG 2761 AGGAAGAGGA GGAAGAGGCT GCCCTACTTC TTCAGCGACG TGAGCCTGGC CGCGCTAGCC 2821 GGCAGTCGAG ATCCCGCCAC CATGGTGAGC AAGGGCGAGG AGGTCATCAA AGAGTTCATG 2881 CGCTTCAAGG TGCGCATGGA GGGCTCCATG AACGGCCACG AGTTCGAGAT CGAGGGCGAG 2941 GGCGAGGGCC GCCCCTACGA GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGCGGC 3001 CCCCTGCCCT TCGCCTGGGA CATCCTGTCC CCCCAGTTCA TGTACGGCTC CAAGGCGTAC 3061 GTGAAGCACC CCGCCGACAT CCCCGATTAC AAGAAGCTGT CCTTCCCCGA GGGCTTCAAG 3121 TGGGAGCGCG TGATGAACTT CGAGGACGGC GGTCTGGTGA CCGTGACCCA GGACTCCTCC 3181 CTGCAGGACG GCACGCTGAT CTACAAGGTG AAGATGCGCG GCACCAACTT CCCCCCCGAC 3241 GGCCCCGTAA TGCAGAAGAA GACCATGGGC TGGGAGGCCT CCACCGAGCG CCTGTACCCC 3301 CGCGACGGCG TGCTGAAGGG CGAGATCCAC CAGGCCCTGA AGCTGAAGGA CGGCGGCCAC 3361 TACCTGGTGG AGTTCAAGAC CATCTACATG GCCAAGAAGC CCGTGCAACT GCCCGGCTAC 3421 TACTACGTGG ACACCAAGCT GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA 3481 CAGTACGAGC GCTCCGAGGG CCGCCACCAC CTGTTCCTGG GGCATGGCAC CGGCAGCACC 3541 GGCAGCGGCA GCTCCGGCAC CGCCTCCTCC GAGGACAACA ACATGGCCGT CATCAAAGAG 3601 TTCATGCGCT TCAAGGTGCG CATGGAGGGC TCCATGAACG GCCACGAGTT CGAGATCGAG 3661 GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG CCAAGCTGAA GGTGACCAAG 3721 GGCGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCCC AGTTCATGTA CGGCTCCAAG 3781 GCGTACGTGA AGCACCCCGC CGACATCCCC GATTACAAGA AGCTGTCCTT CCCCGAGGGC 3841 TTCAAGTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGTC TGGTGACCGT GACCCAGGAC 3901 TCCTCCCTGC AGGACGGCAC GCTGATCTAC AAGGTGAAGA TGCGCGGCAC CAACTTCCCC 3961 CCCGACGGCC CCGTAATGCA GAAGAAGACC ATGGGCTGGG AGGCCTCCAC CGAGCGCCTG 4021 TACCCCCGCG ACGGCGTGCT GAAGGGCGAG ATCCACCAGG CCCTGAAGCT GAAGGACGGC 4081 GGCCACTACC TGGTGGAGTT CAAGACCATC TACATGGCCA AGAAGCCCGT GCAACTGCCC 4141 GGCTACTACT ACGTGGACAC CAAGCTGGAC ATCACCTCCC ACAACGAGGA CTACACCATC 4201 GTGGAACAGT ACGAGCGCTC CGAGGGCCGC CACCACCTGT TCCTGTACGG CATGGACGAG 4261 CTGTACAAGT AAGAATTCGA AGCTATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA 4321 ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC 4381 GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC 4441 TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT 4501 GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC 4561 TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC 4621 GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG 4681 GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT 4741 CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC 4801 CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT 4861 CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC GGCCGCTTCC CTTTAGTGAG 4921 GGTTAATGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA 4981 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA 5041 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG 5101 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG 5161 AATTCTTAAT TAACTAGCCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC 5221 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT 5281 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA 5341 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT 5401 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA 5461 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC 5521 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG 5581 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG 5641 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG 5701 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT 5761 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC 5821 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 5881 TTGCTGGCCT TTTGCTCACA TGTTCTTAAT TAAGCTGTAC ACTGTGGAAT GTGTGTCAGT 5941 TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 6001 ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA 6061 GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC 6121 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGACTG ACTAATTTTT TTTATTTATG 6181 CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG 6241 GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC 6301 AGCACGTGTT GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACTCACT 6361 ATAGGAGGGC CACCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG 6421 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG 6481 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG 6541 CCCTGAATGA ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC 6601 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG 6661 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA 6721 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC 6781 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG 6841 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG 6901 CGAGCATGCC CGACGGCGAG GATCTCGTCG TGACACATGG CGATGCCTGC TTGCCGAATA 6961 TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG 7021 ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT 7081 GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT 7141 TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA 7201 AGCGAATTCG CTAGAGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC 7261 CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GTGTTGGGTC GTTTGTTCAT 7321 AAACGCGGGG TTCGGTCCCA GGGCTGGCAC TCTGTCGATA CCCCACCGAG ACCCCATTGG 7381 GGCCAATACG CCCGCGTTTC TTCCTTTTCC CCACCCCACC CCCCAAGTTC GGGTGAAGGC 7441 CCAGGGCTCG CAGCCAACGT CGGGGCGGCA GGCCCTGCCA TAG //