LOCUS PTL2W.TXT 5986 BP DS-DNA CIRCULAR SYN 26-MAR-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Plasmid from Blueheron biotechnology. Used for cloning codon optimized genes into it. FEATURES Location/Qualifiers promoter 113..310 /note="EF-1alpha promoter core" promoter 113..1291 /note="EF1a promoter and UTR" exon 311..343 /note="EF-1a exon 1" intron 344..1283 /note="EF-1a intron A" exon 1283..1291 /note="EF-1a exon 2 leader" CDS 1353..>2753 /note="HPV31 L2 (not codon modified)" mRNA 2803..>3391 /note="WPRE" polyA_signal 3439..>3664 /note="SV40 late polyA" rep_origin 3665..4419 /note="MB1 ori" promoter 4594..4768 /note="SV40 early promoter" repeat_region 4594..4657 /note="SV40 21 bp repeats" rep_origin 4617..4768 /note="SV40 ori" mRNA 4666..4715 /note="SV40 transcription start points" promoter 4803..4858 /note="EM7" CDS 4878..5672 /note="Kan/Neo resistance" polyA_signal 5720..5986 /note="HSV TK pA signal" polyA_site 5778..5796 /note="HSV TK pA site" BASE COUNT 1320 A 1553 C 1567 G 1546 T 0 OTHER ORIGIN - 1 CCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTGCAAAGA TGGATAAAGT TTTAAACAGA 61 GAGGAATCTT TGCAGCTAAT GGACCTTGTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 121 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 181 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 241 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 301 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 361 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 421 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 481 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 541 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 601 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 661 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 721 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 781 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 841 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 901 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 961 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1021 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1081 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1141 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1201 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1261 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA 1321 GATCCATTGT GCTGGATCCA CCGGTCGCCA CCATGAGAAG CAAGAGGAGC ACCAAGAGGA 1381 CCAAGAGGGC CAGCGCTACA CAGTTATATC AAACATGCAA AGCAGCAGGT ACTTGTCCAT 1441 CAGACGTTAT ACCTAAAATA GAACATACTA CCATTGCAGA CCAAATATTA AGGTATGGTA 1501 GTATGGGTGT TTTTTTTGGT GGGTTGGGTA TTGGGTCCGG CTCTGGTACT GGGGGTCGCA 1561 CTGGATATGT CCCTCTTAGT ACACGTCCTT CTACAGTATC TGAGGCAAGT ATACCTATTA 1621 GACCACCAGT TAGCATTGAC CCTGTAGGTC CCTTGGACCC CTCTATAGTA AGTCTTGTTG 1681 AAGAATCTGG AATTGTTGAT GTTGGTGCCC CTGCTCCTAT ACCACACCCT CCTACAACAT 1741 CTGGGTTTGA CATTGCTACA ACTGCAGACA CAACACCTGC AATTTTAGAT GTAACAAGTG 1801 TTAGCACACA TGAAAATCCT ACTTTTACTG ATCCATCTGT ATTGCAGCCT CCTACACCTG 1861 CAGAAACATC AGGTCATTTA CTACTTTCAT CATCATCTAT TAGCACACAT AATTATGAGG 1921 AAATACCTAT GGATACATTT ATTGTTTCTA CTAATAATGA AAACATAACA AGTAGCACAC 1981 CCATTCCAGG GGTGCGCCGT CCTGCACGTT TAGGGTTATA TAGTAAGGCT ACACAACAAG 2041 TAAAAGTTAT TGATCCAACG TTTCTTAGTG CTCCAAAACA GCTAATTACA TATGAAAACC 2101 CTGCCTATGA AACTGTAAAT GCTGAAGAAT CTTTATACTT TTCCAATACA TCGCATAATA 2161 TAGCCCCTGA TCCCGACTTT CTAGATATTA TAGCATTACA TAGGCCTGCC CTTACCTCAC 2221 GTAGGAACAC TGTTAGATAT AGTAGACTAG GTAATAAACA AACTTTGCGC ACTCGTAGTG 2281 GTGCTACTAT TGGTGCAAGG GTGCATTATT ATTATGATAT TAGTAGTATT AATCCTGCAG 2341 GTGAAAGTAT TGAAATGCAA CCTTTAGGGG CGTCTGCAAC TACTACTTCT ACTTTAAATG 2401 ATGGCTTATA TGACATTTAT GCAGACACTG ATTTTACTGT GGATACACCT GCCACACATA 2461 ATGTTTCCCC TTCTACTGCT GTACAGTCCA CATCTGCTGT GTCTGCCTAT GTACCTACAA 2521 ATACCACTGT GCCACTAAGT ACAGGTTTTG ACATTCCCAT ATTTTCTGGG CCTGATGTAC 2581 CTATAGAGCA TGCACCTACA CAGGTTTTCC CATTTCCTTT GGCCCCTACA ACGCCACAAG 2641 TGTCTATTTT TGTTGATGGG GGTGATTTTT ATTTGCACCC TAGCTACTAT ATGCTGAAAC 2701 GCCGACGCAA ACGCGTCAGC TACTTCTTCA CCGACGTTAG CGTCGCCGCA TGAGACTAGT 2761 GGATCCCCCG GGCTGCAGGA ATTCGATATC AAGCTTATCG ATAATCAACC TCTGGATTAC 2821 AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA 2881 TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC 2941 TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA 3001 CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC 3061 ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC 3121 ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC 3181 GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG 3241 ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT 3301 TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG 3361 AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CATCGATACC GTCGGCCGCT TCCCTTTAGT 3421 GAGGGTTAAT GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA 3481 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG 3541 TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC 3601 AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA 3661 TGGAATTCTT AATTAACTAG CCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG 3721 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT 3781 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA 3841 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC 3901 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC 3961 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT 4021 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG 4081 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA 4141 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT 4201 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA 4261 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC 4321 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC 4381 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT AATTAAGCTG TACACTGTGG AATGTGTGTC 4441 AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 4501 TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 4561 AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC 4621 CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGA CTGACTAATT TTTTTTATTT 4681 ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT 4741 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG 4801 ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACTC 4861 ACTATAGGAG GGCCACCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT 4921 GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG 4981 CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG 5041 GTGCCCTGAA TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG 5101 TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG 5161 GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA 5221 TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC 5281 ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC 5341 AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA 5401 AGGCGAGCAT GCCCGACGGC GAGGATCTCG TCGTGACACA TGGCGATGCC TGCTTGCCGA 5461 ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG 5521 CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG 5581 AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG 5641 CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA 5701 CCAAGCGAAT TCGCTAGAGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA 5761 ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGTGTTGG GTCGTTTGTT 5821 CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT 5881 TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA 5941 GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAG //