LOCUS PTL1W.TXT 6100 BP DS-DNA CIRCULAR SYN 26-MAR-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Plasmid from Blueheron biotechnology. Used for cloning codon optimized genes into it. FEATURES Location/Qualifiers promoter 113..310 /note="EF-1alpha promoter core" promoter 113..1291 /note="EF1a promoter and UTR" exon 311..343 /note="EF-1a exon 1" intron 344..1283 /note="EF-1a intron A" exon 1283..1291 /note="EF-1a exon 2 leader" CDS 1353..>2867 /note="HPV31 L1 (not codon modified)" mRNA 2917..>3505 /note="WPRE" polyA_signal 3553..>3778 /note="SV40 late polyA" rep_origin 3779..4533 /note="MB1 ori" promoter 4708..4882 /note="SV40 early promoter" repeat_region 4708..4771 /note="SV40 21 bp repeats" rep_origin 4731..4882 /note="SV40 ori" mRNA 4780..4829 /note="SV40 transcription start points" promoter 4917..4972 /note="EM7" CDS 4992..5786 /note="Kan/Neo resistance" polyA_signal 5834..6100 /note="HSV TK pA signal" polyA_site 5892..5910 /note="HSV TK pA site" BASE COUNT 1398 A 1500 C 1601 G 1601 T 0 OTHER ORIGIN - 1 CCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTGCAAAGA TGGATAAAGT TTTAAACAGA 61 GAGGAATCTT TGCAGCTAAT GGACCTTGTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 121 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 181 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 241 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 301 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 361 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 421 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 481 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 541 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 601 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 661 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 721 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 781 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 841 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 901 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 961 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1021 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1081 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1141 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1201 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1261 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA 1321 GATCCATTGT GCTGGATCCA CCGGTCGCCA CCATGAGCCT CTGGAGACCC TCTGAAGCTA 1381 CTGTCTACTT ACCACCTGTC CCAGTGTCTA AAGTTGTAAG CACGGATGAA TATGTAACAC 1441 GAACCAACAT ATATTATCAC GCAGGCAGTG CTAGGCTGCT TACAGTAGGC CATCCATATT 1501 ATTCCATACC TAAATCTGAC AATCCTAAAA AAATAGTTGT ACCAAAGGTG TCAGGATTAC 1561 AATATAGGGT ATTTAGGGTT CGTTTACCAG ATCCAAACAA ATTTGGATTT CCTGATACAT 1621 CTTTTTATAA TCCTGAAACT CAACGCTTAG TTTGGGCCTG TGTTGGTTTA GAGGTAGGTC 1681 GCGGGCAGCC ATTAGGTGTA GGTATTAGTG GTCATCCATT ATTAAATAAA TTTGATGACA 1741 CTGAAAACTC TAATAGATAT GCCGGTGGTC CTGGCACTGA TAATAGGGAA TGTATATCAA 1801 TGGATTATAA ACAAACACAA CTGTGTTTAC TTGGTTGCAA ACCACCTATT GGAGAGCATT 1861 GGGGTAAAGG TAGTCCTTGT AGTAACAATG CTATTACCCC TGGTGATTGT CCTCCATTAG 1921 AATTAAAAAA TTCAGTTATA CAAGATGGGG ATATGGTTGA TACAGGCTTT GGAGCTATGG 1981 ATTTTACTGC TTTACAAGAC ACTAAAAGTA ATGTTCCTTT GGACATTTGT AATTCTATTT 2041 GTAAATATCC AGATTATCTT AAAATGGTTG CTGAGCCATA TGGCGATACA TTATTTTTTT 2101 ATTTACGTAG GGAACAAATG TTTGTAAGGC ATTTTTTTAA TAGATCAGGC ACGGTTGGTG 2161 AATCGGTCCC TACTGACTTA TATATTAAAG GCTCCGGTTC AACAGCTACT TTAGCTAACA 2221 GTACATACTT TCCTACACCT AGCGGCTCCA TGGTTACTTC AGATGCACAA ATTTTTAATA 2281 AACCATATTG GATGCAACGT GCTCAGGGAC ACAATAATGG TATTTGTTGG GGCAATCAGT 2341 TATTTGTTAC TGTGGTAGAT ACCACACGTA GTACCAATAT GTCTGTTTGT GCTGCAATTG 2401 CAAACAGTGA TACTACATTT AAAAGTAGTA ATTTTAAAGA GTATTTAAGA CATGGTGAGG 2461 AATTTGATTT ACAATTTATA TTTCAGTTAT GCAAAATAAC ATTATCTGCA GACATAATGA 2521 CATATATTCA CAGTATGAAT CCTGCTATTT TGGAAGATTG GAATTTTGGA TTGACCACAC 2581 CTCCCTCAGG TTCTTTGGAG GATACCTATA GGTTTGTCAC CTCACAGGCC ATTACATGTC 2641 AAAAAACTGC CCCCCAAAAG CCCAAGGAAG ATCCATTTAA AGATTATGTA TTTTGGGAGG 2701 TTAATTTAAA AGAAAAGTTT TCTGCAGATT TAGATCAGTT TCCACTGGGT CGCAAATTTT 2761 TATTACAGGC AGGATATAGG GCACGTCCTA AATTTAAAGC AGGTAAACGT AGTGCACCCT 2821 CAGCATCTAC CACTACACCA GCAAAGAGGA AGAAGACCAA GAAATGAGAC TAGTGGATCC 2881 CCCGGGCTGC AGGAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT 2941 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT 3001 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG 3061 TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC 3121 GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT 3181 CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC 3241 GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG 3301 TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG 3361 CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC 3421 GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG 3481 ATCTCCCTTT GGGCCGCCTC CCCGCATCGA TACCGTCGGC CGCTTCCCTT TAGTGAGGGT 3541 TAATGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA 3601 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA 3661 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC 3721 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGAAT 3781 TCTTAATTAA CTAGCCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 3841 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 3901 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 3961 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT 4021 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 4081 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 4141 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 4201 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 4261 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 4321 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 4381 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 4441 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 4501 CTGGCCTTTT GCTCACATGT TCTTAATTAA GCTGTACACT GTGGAATGTG TGTCAGTTAG 4561 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT 4621 AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 4681 TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA 4741 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGACTGACT AATTTTTTTT ATTTATGCAG 4801 AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG 4861 GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAGC 4921 ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA GTATAATACG ACTCACTATA 4981 GGAGGGCCAC CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG 5041 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT 5101 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC 5161 TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT 5221 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG 5281 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG 5341 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG 5401 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG 5461 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA 5521 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CACATGGCGA TGCCTGCTTG CCGAATATCA 5581 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC 5641 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG 5701 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT 5761 ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC 5821 GAATTCGCTA GAGGGAGGCT AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC 5881 GCTATGACGG CAATAAAAAG ACAGAATAAA ACGCACGGTG TTGGGTCGTT TGTTCATAAA 5941 CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC 6001 CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA 6061 GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG //