LOCUS PRSV-TEX.G 5755 BP DS-DNA CIRCULAR SYN 27-APR-1993 DEFINITION pSV2-Cat cloning vector. ACCESSION M77788 VERSION M77788.1 GI:209172 KEYWORDS . SOURCE Synthetic construct DNA. ORGANISM synthetic construct artificial sequence. REFERENCE 1 (bases 1 to 5003) AUTHORS Gilbert,W. TITLE Obtained from VecBase 3.0 JOURNAL Unpublished (1991) COMMENT These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. pSV2-Cat - Cloning Vector for mammalian cells ENTRY PSV2CAT #TYPE DNA CIRCULAR TITLE pSV2-Cat - Cloning Vector for mammalian cells DATE 06-JUN-1986 #sequence 16-DEC-1986 ACCESSION VB0065 SOURCE artificial COLLECTION ATCC 37155 REFERENCE #number 1 #authors Gorman C. #book 'DNA cloning, Volume II, a practical approach', pp.143-190 edited by D. M. Glover, IRL Press, (1986) Washington, D.C. and Oxford, Rickwood, D., and Hames, B. D., eds. REFERENCE #number 2 #authors Pouwels P.H., Enger-Valk B.E., Brammar W.J. #book Cloning Vectors, Elsvier 1985 and supplements #comment vector VIII-C-a-i-4 COMMENT Entered by William Gilbert, Whitaker College, MIT, 02-APR-1986. Revised 16-DEC-1986 by F. Pfeiffer: 988/9 'AT' to 'TA' to match revised sequence of PBR322 COMMENT 177-234 and 232-303 'tandem' repeat of 72bp SV40 enhancer KEYWORDS CROSSREFERENCE #parent VecBase(3):pBR322, VecBase(3):pBR325, GenBank(50):SV4CG PARENT Features of pSV2Cat (5003 bp) residue source 1- 70 5174-5243 SV40 71- 234 1- 164 SV40 230- 328 177- 275 SV40 323-2620 2066-4363 pBR322 2617-3610 1781-2774 SV40 3607-4220 4100-4713 SV40 4224-4998 5311-4537 (c) pBR325 Conflict (cfl) and Mutations (mut): pSV2Cat source cfl 4385 G A 5150 (c) pBR325 FEATURE 1555-2343 789-1 (c) Ap-R; b-lactamase 4310-4969 660-1 (c) Cm-R; Chloramphenicol-acetyltransferase POLYLINKER SELECTION #resistance Ap, Cm SUMMARY pSV2Cat #length 5003 #checksum 1260. FEATURES Location/Qualifiers misc_feature 1..56 /note="linker sequence identified by sequencing" source 1..1 /note="Provided by Mary Loekin, cite Cell 55:1171" CDS 57..2183 /note="SV40 T antigen" misc_feature complement(2234..<3121) /note="SV40 homology" polyA_signal 2234..2315 /note="SV40 pA" CDS complement(2309..3121) /note="last 3/4ths of VP1 ORF" misc_feature 2872..2872 /note="T2064R Sequencing OK to here" misc_feature <3118..5233 /note="pBR322 backbone" CDS 3326..4183 /note="AmpR" rep_origin 4336..5005 /note="pBR322 Ori (low copy number)" misc_feature 5124..5124 /note="T52L sequencing OK back to here" promoter 5234..>5755 /note="RSV promoter insert" misc_feature 5513..5513 /note="A -> T according to sequencing" misc_feature 5640..5640 /note="A -> G according to sequencing" misc_feature 5689..5689 /note="A missing here according to sequencing" BASE COUNT 1658 A 1161 C 1352 G 1584 T 0 OTHER ORIGIN - 1 CCAAGCTTGG GCTGCAGGTC GACAGATCCC GCGACCGGTC GACCAGCTTT GCAAAGATGG 61 ATAAAGTTTT AAACAGAGAG GAATCTTTGC AGCTAATGGA CCTTCTAGGT CTTGAAAGGA 121 GTGCCTGGGG GAATATTCCT CTGATGAGAA AGGCATATTT AAAAAAATGC AAGGAGTTTC 181 ATCCTGATAA AGGAGGAGAT GAAGAAAAAA TGAAGAAAAT GAATACTCTG TACAAGAAAA 241 TGGAAGATGG AGTAAAATAT GCTCATCAAC CTGACTTTGG AGGCTTCTGG GATGCAACTG 301 AGATTCCAAC CTATGGAACT GATGAATGGG AGCAGTGGTG GAATGCCTTT AATGAGGAAA 361 ACCTGTTTTG CTCAGAAGAA ATGCCATCTA GTGATGATGA GGCTACTGCT GACTCTCAAC 421 ATTCTACTCC TCCAAAAAAG AAGAGAAAGG TAGAAGACCC CAAGGACTTT CCTTCAGAAT 481 TGCTAAGTTT TTTGAGTCAT GCTGTGTTTA GTAATAGAAC TCTTGCTTGC TTTGCTATTT 541 ACACCACAAA GGAAAAAGCT GCACTGCTAT ACAAGAAAAT TATGGAAAAA TATTCTGTAA 601 CCTTTATAAG TAGGCATAAC AGTTATAATC ATAACATACT GTTTTTTCTT ACTCCACACA 661 GGCATAGAGT GTCTGCTATT AATAACTATG CTCAAAAATT GTGTACCTTT AGCTTTTTAA 721 TTTGTAAAGG GGTTAATAAG GAATATTTGA TGTATAGTGC CTTGACTAGA GATCCATTTT 781 CTGTTATTGA GGAAAGTTTG CCAGGTGGGT TAAAGGAGCA TGATTTTAAT CCAGAAGAAG 841 CAGAGGAAAC TAAACAAGTG TCCTGGAAGC TTGTAACAGA GTATGCAATG GAAACAAAAT 901 GTGATGATGT GTTGTTATTG CTTGGGATGT ACTTGGAATT TCAGTACAGT TTTGAAATGT 961 GTTTAAAATG TATTAAAAAA GAACAGCCCA GCCACTATAA GTACCATGAA AAGCATTATG 1021 CAAATGCTGC TATATTTGCT GACAGCAAAA ACCAAAAAAC CATATGCCAA CAGGCTGTTG 1081 ATACTGTTTT AGCTAAAAAG CGGGTTGATA GCCTACAATT AACTAGAGAA CAAATGTTAA 1141 CAAACAGATT TAATGATCTT TTGGATAGGA TGGATATAAT GTTTGGTTCT ACAGGCTCTG 1201 CTGACATAGA AGAATGGATG GCTGGAGTTG CTTGGCTACA CTGTTTGTTG CCCAAAATGG 1261 ATTCAGTGGT GTATGACTTT TTAAAATGCA TGGTGTACAA CATTCCTAAA AAAAGATACT 1321 GGCTGTTTAA AGGACCAATT GATAGTGGTA AAACTACATT AGCAGCTGCT TTGCTTGAAT 1381 TATGTGGGGG GAAAGCTTTA AATGTTAATT TGCCCTTGGA CAGGCTGAAC TTTGAGCTAG 1441 GAGTAGCTAT TGACCAGTTT TTAGTAGTTT TTGAGGATGT AAAGGGCACT GGAGGGGAGT 1501 CCAGAGATTT GCCTTCAGGT CAGGGAATTA ATAACCTGGA CAATTTAAGG GATTATTTGG 1561 ATGGCAGTGT TAAGGTAAAC TTAGAAAAGA AACACCTAAA TAAAAGAACT CAAATATTTC 1621 CCCCTGGAAT AGTCACCATG AATGAGTACA GTGTGCCTAA AACACTGCAG GCCAGATTTG 1681 TAAAACAAAT AGATTTTAGG CCCAAAGATT ATTTAAAGCA TTGCCTGGAA CGCAGTGAGT 1741 TTTTGTTAGA AAAGAGAATA ATTCAAAGTG GCATTGCTTT GCTTCTTATG TTAATTTGGT 1801 ACAGACCTGT GGCTGAGTTT GCTCAAAGTA TTCAGAGCAG AATTGTGGAG TGGAAAGAGA 1861 GATTGGACAA AGAGTTTAGT TTGTCAGTGT ATCAAAAAAT GAAGTTTAAT GTGGCTATGG 1921 GAATTGGAGT TTTAGATTGG CTAAGAAACA GTGATGATGA TGATGAAGAC AGCCAGGAAA 1981 ATGCTGATAA AAATGAAGAT GGTGGGGAGA AGAACATGGA AGACTCAGGG CATGAAACAG 2041 GCATTGATTC ACAGTCCCAA GGCTCATTTC AGGCCCCTCA GTCCTCACAG TCTGTTCATG 2101 ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC 2161 CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACGG GGATCTGTCG 2221 ACTCTAGAGG ATCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT 2281 CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT 2341 CATCAATGTA TCTTATCATG TCTGGATCCC CAGGAAGCTC CTCTGTGTCC TCATAAACCC 2401 TAACCTCCTC TACTTGAGAG GACATTCCAA TCATAGGCTG CCCATCCACC CTCTGTGTCC 2461 TCCTGTTAAT TAGGTCACTT AACAAAAAGG AAATTGGGTA GGGGTTTTTC ACAGACCGCT 2521 TTCTAAGGGT AATTTTAAAA TATCTGGGAA GTCCCTTCCA CTGCTGTGTT CCAGAAGTGT 2581 TGGTAAACAG CCCACAAATG TCAACAGCAG AAACATACAA GCTGTCAGCT TTGCACAAGG 2641 GCCCAACACC CTGCTCATCA AGAAGCACTG TGGTTGCTGT GTTAGTAATG TGCAAAACAG 2701 GAGGCACATT TTCCCCACCT GTGTAGGTTC CAAAATATCT AGTGTTTTCA TTTTTACTTG 2761 GATCAGGAAC CCAGCACTCC ACTGGATAAG CATTATCCTT ATCCAAAACA GCCTTGTGGT 2821 CAGTGTTCAT CTGCTGACTG TCAACTGTAG CATTTTTTGG GGTTACAGTT TGAGCAGGAT 2881 ATTTGGTCCT GTAGTTTGCT AACACACCCT GCAGCTCCAA AGGTTCCCCA CCAACAGCAA 2941 AAAAATGAAA ATTTGACCCT TGAATGGGTT TTCCAGCACC ATTTTCATGA GTTTTTTGTG 3001 TCCCTGAATG CAAGTTTAAC ATAGCAGTTA CCCCAATAAC CTCAGTTTTA ACAGTAACAG 3061 CTTCCCACAT CAAAATATTT CCACAGGTTA AGTCCTCATT TAAATTAGGC AAAGGAATTC 3121 TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT 3181 GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT 3241 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT 3301 TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC 3361 CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA 3421 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG 3481 TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT 3541 TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG 3601 CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC 3661 GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC 3721 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA 3781 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC 3841 AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT 3901 AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA 3961 TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA 4021 ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA 4081 GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA 4141 TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT 4201 TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT 4261 GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG 4321 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT 4381 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA 4441 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC 4501 TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC 4561 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT 4621 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG 4681 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA 4741 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT 4801 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA 4861 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC 4921 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC 4981 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA 5041 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG 5101 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT 5161 GTGCGGTATT TCACACCGCA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA 5221 GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA 5281 AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGGGT 5341 TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTATCT GAGGGGACTA 5401 GGGTGTGTTT AGGCGAAAAG CGGGGCTTCG GTTGTACGCG GTTAGGAGTC CCCTCAGGAT 5461 ATAGTAGTTT CGCTTTTGCA TAGGGAGGGG GAAATGTAGT CTTATGCAAT ACTCTTGTAG 5521 TCTTGCAACA TGGTAACGAT GAGTTAGCAA CATGCCTTAC AAGGAGAGAA AAAGCACCGT 5581 GCATGCCGAT TGGTGGAAGT AAGGTGGTAC GATCGTGCCT TATTAGGAAG GCAACAGACG 5641 GGTCTGACAT GGATTGGACG AACCACTGAA TTCCGCATTG CAGAGATATT GTATTTAAGT 5701 GCCTAGCTCG ATACAATAAA CGCCATTTGA CCATTCACCA CATTGGTGTG CACCT //