LOCUS PU-GFP (LA 3869 BP DS-DNA CIRCULAR SYN 25-FEB-1997 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers misc_feature 209..>227 /note="small piece of CMV promoter" promoter <228..>911 /note="URR fragment" protein_bind 241..252 /note="E2 BS (high affinity)" protein_bind 284..295 /note="E2 BS" repeat_region 335..346 /note="possible E2BS" protein_bind 386..397 /note="E2 BS" protein_bind 467..478 /note="E2 BS" protein_bind 496..507 /note="E2 BS (high affinity)" protein_bind 510..521 /note="E2 BS" mutation 638..638 /note="extra C discovered by sequencing" protein_bind 657..668 /note="E2 BS (high affinity)" protein_bind 772..783 /note="E2 BS" rep_origin 772..849 /note="Minimal Ori (Fields p2211)" protein_bind 772..783 /note="E2 BS11 (high affinity, technically dispensible for replication)" misc_feature 784..816 /note="A-T rich region" misc_feature 817..822 /note="Dyad Symmetry repeats" misc_feature 823..834 /note="Dyad Symmetry repeats" protein_bind 838..849 /note="E2 BS12" TATA_signal 880..885 /note="P89 TATA" protein_bind 895..906 /note="possible E2BS" misc_RNA 909..>915 /note="P89 cap site (Type 1)" CDS 951..1670 /note="EGFP (weighs 27kD)" polyA_signal 1824..1829 /note="pA signal" polyA_signal 1824..1874 /note="SV40 polyA " polyA_signal 1853..1858 /note="pA signal" mRNA 1862..1862 /note="mRNA 3' end" mRNA 1874..1874 /note="mRNA 3' end" rep_origin 2055..2728 /note="ColE1 Ori" CDS complement(2873..3733) /note="AmpR" promoter complement(3768..3803) /note="AmpR promoter" BASE COUNT 999 A 1006 C 941 G 923 T 0 OTHER ORIGIN - 1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTGCAAAGC 241 ACCGGCGGCG GTAGATGCGG GGTAAGTACT GAATTTTAAT TCGACCTATC CCGGTAAAGC 301 GAAAGCGACA CGCTTTTTTT TCACACATAG CGGGACCGAA CACGTTATAA GTATCGATTA 361 GGTCTATTTT TGTCTCTCTG TCGGAACCAG AACTGGTAAA AGTTTCCATT GCGTCTGGGC 421 TTGTCTATCA TTGCGTCTCT ATGGTTTTTG GAGGATTAGA CGGGCCACCA GTAATGGTGC 481 ATAGCGGATG TCTGTACCGC CATCGGTGCA CCGATATAGG TTTGGGGCTC CCCAAGGGAC 541 TGCTGGGATG ACAGCTTCAT ATTATATTGA ATGGGCGCAT AATCAGCTTA ATTGGTGAGG 601 ACAAGCTACA AGTTGTAACC TGATCTCCAC AAAGTACCGT TGCCGGTCGG GGTCAAACCG 661 TCTTCGGTGC TCGAAACCGC CTTAAACTAC AGACAGGTCC CAGCCAAGTA GGCGGATCAA 721 AACCTCAAAA AGGCGGGAGC CAATCAAAAT GCAGCATTAT ATTTTAAGCT CACCGAAACC 781 GGTAAGTAAA GACTATGTAT TTTTTCCCAG TGAATAATTG TTGTTAACAA TAATCACACC 841 ATCACCGTTT TTTCAAGCGG GAAAAAATAG CCAGCTAACT ATAAAAAGCT GCTGACAGAC 901 CCCGGTTTTC ATTAAGCTTG GTACCGAGCT CGGATCCACC GGTCGCCACC ATGGTGAGCA 961 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA 1021 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA 1081 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA 1141 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT 1201 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG 1261 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA 1321 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT 1381 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG 1441 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC 1501 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA 1561 CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT 1621 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA AGCGGCCGCG 1681 ACTCTAGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT 1741 CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT 1801 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC 1861 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAAGGCGT 1921 AAATTGTAAG CGTTAATGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 1981 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 2041 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 2101 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 2161 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 2221 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 2281 TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT 2341 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 2401 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 2461 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 2521 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 2581 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 2641 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 2701 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 2761 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 2821 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 2881 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 2941 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 3001 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 3061 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 3121 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 3181 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 3241 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 3301 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 3361 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 3421 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 3481 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 3541 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 3601 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 3661 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 3721 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 3781 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 3841 CATTTCCCCG AAAAGTGCCA CCTGACGTC //