LOCUS PU-7452 (L 7452 BP DS-DNA CIRCULAR SYN 10-MAR-1998 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers CDS complement(<229..1739) /note="LacZ [Split]" frag 229..1841 /note="4619 to 6231 of pSC11 MCS2 (annot)" promoter <1857..>2540 /note="URR fragment [Split]" rep_origin 2401..2478 /note="Minimal Ori (Fields p2211)" misc_RNA 2538..>2544 /note="P89 cap site (Type 1)" polyA_signal 2574..2843 /note="HSV TK pA signal" polyA_site 2632..2650 /note="HSV TK pA sites" promoter 2956..4134 /note="EF1a promoter" enhancer 2956..3153 /note="EF-1alpha promoter core domain" exon 3154..3186 /note="EF-1a exon 1" intron 3187..4126 /note="EF-1a intron A" exon 4126..4134 /note="EF-1a exon 2 leader" CDS 4195..4911 /note="EGFP (weighs 27kD)" polyA_signal 4926..5203 /note="BGH polyA" rep_origin 5633..6306 /note="Coli Ori" CDS complement(6458..7316) /note="AmpR" promoter complement(7351..7386) /note="AmpR's promoter" BASE COUNT 1729 A 1979 C 2011 G 1733 T 0 OTHER ORIGIN - 1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGCGTACATC 241 GGGCAAATAA TATCGGTGGC CGTGGTGTCG GCTCCGCCGC CTTCATACTG CACCGGGCGG 301 GAAGGATCGA CAGATTTGAT CCAGCGATAC AGCGCGTCGT GATTAGCGCC GTGGCCTGAT 361 TCATTCCCCA GCGACCAGAT GATCACACTC GGGTGATTAC GATCGCGCTG CACCATTCGC 421 GTTACGCGTT CGCTCATCGC CGGTAGCCAG CGCGGATCAT CGGTCAGACG ATTCATTGGC 481 ACCATGCCGT GGGTTTCAAT ATTGGCTTCA TCCACCACAT ACAGGCCGTA GCGGTCGCAC 541 AGCGTGTACC ACAGCGGATG GTTCGGATAA TGCGAACAGC GCACGGCGTT AAAGTTGTTC 601 TGCTTCATCA GCAGGATATC CTGCACCATC GTCTGCTCAT CCATGACCTG ACCATGCAGA 661 GGATGATGCT CGTGACGGTT AACGCCTCGA ATCAGCAACG GCTTGCCGTT CAGCAGCAGC 721 AGACCATTTT CAATCCGCAC CTCGCGGAAA CCGACATCGC AGGCTTCTGC TTCAATCAGC 781 GTGCCGTCGG CGGTGTGCAG TTCAACCACC GCACGATAGA GATTCGGGAT TTCGGCGCTC 841 CACAGTTTCG GGTTTTCGAC GTTGAGACGT AGTGTGACGC GATCGGCATA ACCACCACGC 901 TCATCGATAA TTTCACCGCC GAAAGGCGCG GTGCCGCTGG CGACCTGCGT TTCACCCTGC 961 CATAAAGAAA CTGTTACCCG TAGGTAGTCA CGCAACTCGC CGCACATCTG AACTTCAGCC 1021 TCCAGTACAG CGCGGCTGAA ATCATCATTA AAGCGAGTGG CAACATGGAA ATCGCTGATT 1081 TGTGTAGTCG GTTTATGCAG CAACGAGACG TCACGGAAAA TGCCGCTCAT CCGCCACATA 1141 TCCTGATCTT CCAGATAACT GCCGTCACTC CAACGCAGCA CCATCACCGC GAGGCGGTTT 1201 TCTCCGGCGC GTAAAAATGC GCTCAGGTCA AATTCAGACG GCAAACGACT GTCCTGGCCG 1261 TAACCGACCC AGCGCCCGTT GCACCACAGA TGAAACGCCG AGTTAACGCC ATCAAAAATA 1321 ATTCGCGTCT GGCCTTCCTG TAGCCAGCTT TCATCAACAT TAAATGTGAG CGAGTAACAA 1381 CCCGTCGGAT TCTCCGTGGG AACAAACGGC GGATTGACCG TAATGGGATA GGTTACGTTG 1441 GTGTAGATGG GCGCATCGTA ACCGTGCATC TGCCAGTTTG AGGGGACGAC GACAGTATCG 1501 GCCTCAGGAA GATCGCACTC CAGCCAGCTT TCCGGCACCG CTTCTGGTGC CGGAAACCAG 1561 GCAAAGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC 1621 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA 1681 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG GATCCCTCGA GGAATTCATT 1741 TATAGCATAG AAAAAAACAA AATGAAATTC TACTATATTT TTACATACAT ATATTCTAAA 1801 TATGAAAGTG GTGATTGTGA CTAGCGTAGC ATCGCTTCTA GTTAGTTATT ACTAGCCGCG 1861 TGCAAAGCAC CGGCGGCGGT AGATGCGGGG TAAGTACTGA ATTTTAATTC GACCTATCCC 1921 GGTAAAGCGA AAGCGACACG CTTTTTTTTC ACACATAGCG GGACCGAACA CGTTATAAGT 1981 ATCGATTAGG TCTATTTTTG TCTCTCTGTC GGAACCAGAA CTGGTAAAAG TTTCCATTGC 2041 GTCTGGGCTT GTCTATCATT GCGTCTCTAT GGTTTTTGGA GGATTAGACC GGGCCACCAG 2101 TAATGGTGCA TAGCGGATGT CTGTACCGCC ATCGGTGCAC CGATATAGGT TTGGGGCTCC 2161 CCAAGGGACT GCTGGGATGA CAGCTTCATA TTATATTGAA TGGGCGCATA ATCAGCTTAA 2221 TTGGTGAGGA CAAGCTACAA GTTGTAACCT GATCTCCACA AAGTACCGTT GCCGGTCGGG 2281 GTCAAACCGT CTTCGGTGCT CGAAACCGCC TTAAACTACA GACAGGTCCC AGCCAAGTAG 2341 GCGGATCAAA ACCTCAAAAA GGCGGGAGCC AATCAAAATG CAGCATTATA TTTTAAGCTC 2401 ACCGAAACCG GTAAGTAAAG ACTATGTATT TTTTCCCAGT GAATAATTGT TGTTAACAAT 2461 AATCACACCA TCACCGTTTT TTCAAGCGGG AAAAAATAGC CAGCTAACTA TAAAAAGCTG 2521 CTGACAGACC CCGGTTTTCA TTAAGCTTGG TACCGAGCTC GGATCCACTA GGGGGAGGCT 2581 AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG 2641 ACAGAATAAA ACGCACGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG 2701 CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC 2761 CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG 2821 GGCGGCAGGC CCTGCCATAG CCTCAGCTAC CGGACTCAGA TCTCGAGCTC AAGCTTGCAA 2881 AGATGGATAA AGTTTTAAAC AGAGAGGAAT CTTTGCAGCT AATGGACCTT GTAGGTCTTG 2941 AAAGGAGTGG GAATTGGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC 3001 CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG 3061 GTAAACTGGG AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA 3121 CCGTATATAA GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA 3181 ACACAGGTAA GTGCCGTGTG TGGTTCCCGC GGGCCTGGCC TCTTTACGGG TTATGGCCCT 3241 TGCGTGCCTT GAATTACTTC CACCTGGCTG CAGTACGTGA TTCTTGATCC CGAGCTTCGG 3301 GTTGGAAGTG GGTGGGAGAG TTCGAGGCCT TGCGCTTAAG GAGCCCCTTC GCCTCGTGCT 3361 TGAGTTGAGG CCTGGCCTGG GCGCTGGGGC CGCCGCGTGC GAATCTGGTG GCACCTTCGC 3421 GCCTGTCTCG CTGCTTTCGA TAAGTCTCTA GCCATTTAAA ATTTTTGATG ACCTGCTGCG 3481 ACGCTTTTTT TCTGGCAAGA TAGTCTTGTA AATGCGGGCC AAGATCTGCA CACTGGTATT 3541 TCGGTTTTTG GGGCCGCGGG CGGCGACGGG GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG 3601 AGGCGGGGCC TGCGAGCGCG GCCACCGAGA ATCGGACGGG GGTAGTCTCA AGCTGGCCGG 3661 CCTGCTCTGG TGCCTGGCCT CGCGCCGCCG TGTATCGCCC CGCCCTGGGC GGCAAGGCTG 3721 GCCCGGTCGG CACCAGTTGC GTGAGCGGAA AGATGGCCGC TTCCCGGCCC TGCTGCAGGG 3781 AGCTCAAAAT GGAGGACGCG GCGCTCGGGA GAGCGGGCGG GTGAGTCACC CACACAAAGG 3841 AAAAGGGCCT TTCCGTCCTC AGCCGTCGCT TCATGTGACT CCACGGAGTA CCGGGCGCCG 3901 TCCAGGCACC TCGATTAGTT CTCGAGCTTT TGGAGTACGT CGTCTTTAGG TTGGGGGGAG 3961 GGGTTTTATG CGATGGAGTT TCCCCACACT GAGTGGGTGG AGACTGAAGT TAGGCCAGCT 4021 TGGCACTTGA TGTAATTCTC CTTGGAATTT GCCCTTTTTG AGTTTGGATC TTGGTTCATT 4081 CTCAAGCCTC AGACAGTGGT TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAGAATTC 4141 TGTAGAGATC CCTCGACCTC GAGATCCATT GTGCTGGATC CACCGGTCGC CACCATGGTG 4201 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC 4261 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG 4321 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG 4381 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC 4441 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG 4501 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC 4561 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG 4621 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC 4681 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC 4741 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG 4801 AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG 4861 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTAAAGCGGC 4921 CCTAGAGCTC GCTGATCAGC CTCGACTGTG CCTCTAGTTG CCAGCCATCT GTTGTTTGCC 4981 CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA 5041 ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG 5101 GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG 5161 GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC GAGTGCATTC TAGTTGTGGT 5221 TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC 5281 TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA 5341 CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA 5401 CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG 5461 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC 5521 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT 5581 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG 5641 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC 5701 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA 5761 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT 5821 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG 5881 GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG 5941 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT 6001 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC 6061 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC 6121 TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC 6181 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT 6241 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC 6301 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 6361 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA 6421 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA 6481 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG 6541 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC 6601 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC 6661 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT 6721 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC 6781 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG 6841 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC 6901 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT 6961 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG 7021 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT 7081 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG 7141 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA 7201 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA 7261 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC 7321 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 7381 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 7441 CCACCTGACG TC //