LOCUS pRwB 5787 bp DNA circular SYN 7-NOV-2006 DEFINITION -. ACCESSION - KEYWORDS -. SOURCE -. FEATURES Location/Qualifiers promoter 21..364 /note="SV40 promoter" misc_RNA complement(21..29) /note="SV40 minor late 19S RNA" enhancer 43..185 /note="SV40 enhancer elements" misc_feature 190..253 /note="SV40 21bp repeats" rep_origin 210..364 /note="SV40 Ori" mRNA 259..265 /note="late-early transcription startpoints" mutation 279..279 /note="G->A kills SELP ATG" mRNA 299..305 /note="early-early transcription start points" misc_feature 364..364 /note="end homology to SV40" promoter 399..453 /note="EM7 bacterial promoter" CDS 473..895 /note="Blasticidin resistance" polyA_signal 925..1146 /note="SV40 Late PolyA" enhancer 1238..1435 /note="EF-1alpha promoter core domain" promoter 1238..2416 /note="EF1a promoter and UTR" exon 1436..1468 /note="EF-1a exon 1" intron 1469..2408 /note="EF-1a intron A" exon 2408..2416 /note="EF-1a exon 2 leader" CDS 2513..3187 /note="dsRed-Express" CDS 3188..3202 /note="artificial tail" mRNA 3246..3834 /note="WPRE" polyA_signal 3870..4538 /note="hEF1a polyA signal" rep_origin 4539..5272 /note="MB1 Ori" promoter 5293..5339 /note="EM7 promoter" CDS 5360..5734 /note="Zeocin resistance" terminator 5735..5787 /note="bacterial terminator (rpmB/G)" BASE COUNT 1283 A 1480 C 1594 G 1430 T 0 OTHER ORIGIN - 1 CCCTGCATCA AGCTTGGTAC CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG 61 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG 121 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG 181 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC 241 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATAC AGAGGCCGAG GCCGCCTCGG 301 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 361 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC 421 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA TCATGAAGAC 481 CTTCAACATC TCTCAGCAGG ATCTGGAGCT GGTGGAGGTC GCCACTGAGA AGATCACCAT 541 GCTCTATGAG GACAACAAGC ACCATGTCGG GGCGGCCATC AGGACCAAGA CTGGGGAGAT 601 CATCTCTGCT GTCCACATTG AGGCCTACAT TGGCAGGGTC ACTGTCTGTG CTGAAGCCAT 661 TGCCATTGGG TCTGCTGTGA GCAACGGGCA GAAGGACTTT GACACCATTG TGGCTGTCAG 721 GCACCCCTAC TCTGATGAGG TGGACAGATC CATCAGGGTG GTCAGCCCCT GTGGCATGTG 781 CAGAGAGCTC ATCTCTGACT ATGCTCCTGA CTGCTTTGTG CTCATTGAGA TGAATGGCAA 841 GCTGGTCAAA ACCACCATTG AGGAACTCAT CCCCCTCAAG TACACCAGGA ACTAAACCTG 901 AATTCGCTAG AGGGCCGCTT CGAGCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA 961 CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT 1021 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA 1081 TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT 1141 GTGGTAAAAT CGATAAGGAT CCGGGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC 1201 CTTCCCAACA GTTGCAGGTG GAGAAGAGCA TGCGTGAGGC TCCGGTGCCC GTCAGTGGGC 1261 AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACCGG 1321 TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCC 1381 TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCTGTGA ACGTTCTTTT 1441 TCGCAACGGG TTTGCCGCCA GAACACAGGT AAGTGCCGTG TGTGGTTCCC GCGGGCCTGG 1501 CCTCTTTACG GGTTATGGCC CTTGCGTGCC TTGAATTACT TCCACCTGGC TGCAGTACGT 1561 GATTCTTGAT CCCGAGCTTC GGGTTGGAAG TGGGTGGGAG AGTTCGAGGC CTTGCGCTTA 1621 AGGAGCCCCT TCGCCTCGTG CTTGAGTTGA GGCCTGGCCT GGGCGCTGGG GCCGCCGCGT 1681 GCGAATCTGG TGGCACCTTC GCGCCTGTCT CGCTGCTTTC GATAAGTCTC TAGCCATTTA 1741 AAATTTTTGA TGACCTGCTG CGACGCTTTT TTTCTGGCAA GATAGTCTTG TAAATGCGGG 1801 CCAAGATCTG CACACTGGTA TTTCGGTTTT TGGGGCCGCG GGCGGCGACG GGGCCCGTGC 1861 GTCCCAGCGC ACATGTTCGG CGAGGCGGGG CCTGCGAGCG CGGCCACCGA GAATCGGACG 1921 GGGGTAGTCT CAAGCTGGCC GGCCTGCTCT GGTGCCTGGC CTCGCGCCGC CGTGTATCGC 1981 CCCGCCCTGG GCGGCAAGGC TGGCCCGGTC GGCACCAGTT GCGTGAGCGG AAAGATGGCC 2041 GCTTCCCGGC CCTGCTGCAG GGAGCTCAAA ATGGAGGACG CGGCGCTCGG GAGAGCGGGC 2101 GGGTGAGTCA CCCACACAAA GGAAAAGGGC CTTTCCGTCC TCAGCCGTCG CTTCATGTGA 2161 CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG TTCTCGAGCT TTTGGAGTAC 2221 GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG TTTCCCCACA CTGAGTGGGT 2281 GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC TCCTTGGAAT TTGCCCTTTT 2341 TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC 2401 ATTTCAGGTG TCGTGAGAAT TCTGTAGAGA TCCCTCGACC TCGAGATCCA TTGTGCTGGA 2461 TCTGCGATCT AAGTAAGCTT CGAATTCTGC AGTCGAGCTA CCGGTCGCCA CCATGGCCTC 2521 CTCCGAGGAC GTCATCAAGG AGTTCATGCG CTTCAAGGTG CGCATGGAGG GCTCCGTGAA 2581 CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC 2641 CGCCAAGCTG AAGGTGACCA AGGGCGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC 2701 CCAGTTCCAG TACGGCTCCA AGGTGTACGT GAAGCACCCC GCCGACATCC CCGACTACAA 2761 GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG 2821 CGTGGTGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC TCCTTCATCT ACAAGGTGAA 2881 GTTCATCGGC GTGAACTTCC CCTCCGACGG CCCCGTAATG CAGAAGAAGA CTATGGGCTG 2941 GGAGGCCTCC ACCGAGCGCC TGTACCCCCG CGACGGCGTG CTGAAGGGCG AGATCCACAA 3001 GGCCCTGAAG CTGAAGGACG GCGGCCACTA CCTGGTGGAG TTCAAGTCCA TCTACATGGC 3061 CAAGAAGCCC GTGCAGCTGC CCGGCTACTA CTACGTGGAC TCCAAGCTGG ACATCACCTC 3121 CCACAACGAG GACTACACCA TCGTGGAGCA GTACGAGCGC GCCGAGGGCC GCCACCACCT 3181 GTTCCTGAGA TCTCGACACT AGTGGATCCC CCGGGCTGCA GGAATTCGAT ATCAAGCTTA 3241 TCGATAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG 3301 TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT 3361 CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG 3421 AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC 3481 CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC 3541 TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC 3601 GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC 3661 TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG 3721 CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC 3781 GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCATCGAT 3841 ACCGTCGGCC CACTGCTCCC TAAACCTGAG CTAGCATTAT CCCTAATACC TGCCACCCCA 3901 CTCTTAATCA GTGGTGGAAG AACGGTCTCA GAACTGTTTG TTTCAATTGG CCATTTAAGT 3961 TTAGTAGTAA AAGACTGGTT AATGATAACA ATGCATCGTA AAACCTTCAG AAGGAAAGGA 4021 GAATGTTTTG TGGACCACTT TGGTTTTCTT TTTTGCGTGT GGCAGTTTTA AGTTATTAGT 4081 TTTTAAAATC AGTACTTTTT AATGGAAACA ACTTGACCAA AAATTTGTCA CAGAATTTTG 4141 AGACCCATTA AAAAAGTTAA ATGAGAAACC TGTGTGTTCC TTTGGTCAAC ACCGAGACAT 4201 TTAGGTGAAA GACATCTAAT TCTGGTTTTA CGAATCTGGA AACTTCTTGA AAATGTAATT 4261 CTTGAGTTAA CACTTCTGGG TGGAGAATAG GGTTGTTTTC CCCCCACATA ATTGGAAGGG 4321 GAAGGAATAT CATTTAAAGC TATGGGAGGG TTTCTTTGAT TACAACACTG GAGAGAAATG 4381 CAGCATGTTG CTGATTGCCT GTCACTAAAA CAGGCCAAAA ACTGAGTCCT TGGGTTGCAT 4441 AGAAAGCTTC ATGTTGCTAA ACCAATGTTA AGTGAATCTT TGGAAACAAA ATGTTTCCAA 4501 ATTACTGGGA TGTGCATGTT GAAACGTGGG TTAATTAACT AGCCATGACC AAAATCCCTT 4561 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT 4621 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG 4681 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA 4741 GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA 4801 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG 4861 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG 4921 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT 4981 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA 5041 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC 5101 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG 5161 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG 5221 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTAATTAAAT 5281 TTTTCAAAAG TAGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC 5341 TCACTATAGG AGGGCCATCA TGGCCAAGTT GACCAGTGCT GTCCCAGTGC TCACAGCCAG 5401 GGATGTGGCT GGAGCTGTTG AGTTCTGGAC TGACAGGTTG GGGTTCTCCA GAGATTTTGT 5461 GGAGGATGAC TTTGCAGGTG TGGTCAGAGA TGATGTCACC CTGTTCATCT CAGCAGTCCA 5521 GGACCAGGTG GTGCCTGACA ACACCCTGGC TTGGGTGTGG GTGAGAGGAC TGGATGAGCT 5581 GTATGCTGAG TGGAGTGAGG TGGTCTCCAC CAACTTCAGG GATGCCAGTG GCCCTGCCAT 5641 GACAGAGATT GGAGAGCAGC CCTGGGGGAG AGAGTTTGCC CTGAGAGACC CAGCAGGCAA 5701 CTGTGTGCAC TTTGTGGCAG AGGAGCAGGA CTGAGGATAA GAATTGTAAC AAAAAACCCC 5761 GCCCCGGCGG GGTTTTTTGT TAATTAA //