LOCUS PRAL1.GB 6076 BP DS-DNA CIRCULAR SYN 26-MAR-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Plasmid from Blueheron biotechnology. Used for cloning codon optimized genes into it. FEATURES Location/Qualifiers promoter 113..310 /note="EF-1alpha promoter core" promoter 113..1291 /note="EF1a promoter and UTR" exon 311..343 /note="EF-1a exon 1" intron 344..1283 /note="EF-1a intron A" exon 1283..1291 /note="EF-1a exon 2 leader" CDS 1352..2836 /note="HPV2 (EF362755) L1 codon modified" mutation 2126..2128 /note="Leu259->Arg relative to HPV2a prototype" mRNA 2893..>3481 /note="WPRE (Tom Hope)" polyA_signal 3529..>3754 /note="SV40 late polyA" rep_origin 3755..4509 /note="MB1 ori" promoter 4684..4858 /note="SV40 early promoter" repeat_region 4684..4747 /note="SV40 21 bp repeats" rep_origin 4707..4858 /note="SV40 ori" mRNA 4756..4805 /note="SV40 transcription start points" promoter 4893..4948 /note="EM7" CDS 4968..5762 /note="Kan/Neo resistance" polyA_signal 5810..6076 /note="HSV TK pA signal" polyA_site 5868..5886 /note="HSV TK pA site" BASE COUNT 1296 A 1774 C 1642 G 1364 T 0 OTHER ORIGIN - 1 CCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTGCAAAGA TGGATAAAGT TTTAAACAGA 61 GAGGAATCTT TGCAGCTAAT GGACCTTGTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 121 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 181 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 241 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 301 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 361 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 421 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 481 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 541 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 601 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 661 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 721 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 781 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 841 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 901 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 961 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1021 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1081 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1141 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1201 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1261 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGAATTCTGT AGAGATCCCT CGACCTCGAG 1321 ATCCATTGTG CTGGATCCAC CGGTCGCCAC CATGGCCCTC TGGCGCCCCA ACGAGTCAAA 1381 GGTCTACCTG CCACCCACCC CAGTCAGCAA AGTCATTTCC ACCGACGTGT ACGTCACCAG 1441 AACCAACGTC TACTATCACG GCGGGTCCAG CCGCTTGCTG ACCGTCGGCC ACCCCTACTA 1501 TAGCATCAAG AAATCCAACA ACAAGGTCGC CGTCCCAAAG GTCTCCGGCT ATCAGTACCG 1561 CGTGTTCCAT GTCAAACTCC CCGACCCCAA CAAGTTCGGG CTCCCTGACG CCGACCTCTA 1621 CGACCCCGAC ACACAACGCT TGCTCTGGGC CTGTGTCGGC GTGGAAGTCG GGAGGGGCCA 1681 GCCCCTCGGC GTCGGCGTCA GCGGCCATCC CTACTACAAC CGCCTCGACG ATACCGAGAA 1741 CGCCCACACC CCCGACACCG CCGACGACGG GCGCGAGAAC ATCAGCATGG ACTACAAGCA 1801 GACCCAACTC TTCATCCTCG GCTGTAAGCC ACCCATCGGC GAACATTGGA GCAAAGGCAC 1861 AACATGCAAC GGCAGCAGCG CCGCCGGCGA TTGTCCCCCA TTGCAGTTCA CCAATACCAC 1921 CATCGAAGAT GGCGACATGG TCGAGACCGG CTTTGGCGCA CTCGACTTCG CAACCCTCCA 1981 AAGCAACAAA AGCGACGTCC CACTCGACAT CTGCACAAAC ACATGCAAGT ACCCCGACTA 2041 CCTCAAGATG GCCGCCGAAC CCTACGGCGA CAGCATGTTC TTCTCGCTCC GCCGCGAGCA 2101 GATGTTTACC CGCCACTTCT TCAACCGCGG GGGCAAGATG GGCGATACAA TTCCCGACGA 2161 ACTGTACATC AAGTCCACAA GCGTCCCCAC CCCCGGGTCC CACGTCTACA CCAGTACCCC 2221 CTCCGGGAGC ATGGTCAGTA GCGAGCAGCA GCTCTTCAAC AAACCCTATT GGTTGAGACG 2281 CGCACAGGGC CATAACAACG GCATGTGTTG GGGCAACCGC GTGTTCCTCA CCGTCGTCGA 2341 TACAACCAGG TCAACCAACG TCAGCCTCTG CGCAACCGAA GCCAGCGACA CCAACTACAA 2401 GGCCACAAAC TTCAAAGAGT ACCTGCGCCA CATGGAAGAG TACGACCTGC AGTTCATCTT 2461 TCAACTCTGT AAGATCACAT TGACCCCCGA GATCATGGCA TACATCCACA ACATGGACCC 2521 ACAGCTCCTG GAAGACTGGA ACTTTGGCGT GCCACCCCCG CCAAGCGCAT CCCTGCAAGA 2581 CACATACCGC TACCTGCAGA GTCAGGCCAT CACCTGCCAG AAGCCCACCC CACCCAAAAC 2641 ACCCACCGAC CCATACGCAA GTCTCACATT CTGGGACGTC GACTTGTCCG AGAGCTTCAG 2701 TATGGACCTC GATCAGTTCC CACTCGGCAG GAAGTTCCTG CTCCAACGCG GCGCCATGCC 2761 CACCGTCAGC AGGAAACGGG CAGCCGTCAG CGGCACAACC CCCCCAACCT CCAAGCGCAA 2821 GAGAGTGCGC CGCTGAGGCT CGAGGCTAGT GGATCCCCCG GGCTGCAGGA ATTCGATATC 2881 AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT 2941 AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT 3001 ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT 3061 TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC 3121 GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT 3181 TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA 3241 GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT 3301 CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC 3361 CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT 3421 CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG 3481 CATCGATACC GTCGGCCGCT TCCCTTTAGT GAGGGTTAAT GCTTCGAGCA GACATGATAA 3541 GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT 3601 GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA 3661 ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT 3721 AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGAATTCTT AATTAACTAG CCATGACCAA 3781 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 3841 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 3901 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 3961 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA 4021 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 4081 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 4141 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 4201 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC 4261 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 4321 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 4381 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 4441 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 4501 AATTAAGCTG TACACTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC 4561 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT 4621 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA 4681 TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC 4741 CGCCCCATGA CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG 4801 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC 4861 CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT AATCATCGGC 4921 ATAGTATATC GGCATAGTAT AATACGACTC ACTATAGGAG GGCCACCATG ATTGAACAAG 4981 ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG 5041 CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC 5101 CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA GACGAGGCAG 5161 CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA 5221 CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT 5281 CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA 5341 CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC 5401 GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC 5461 TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC GAGGATCTCG 5521 TCGTGACACA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG 5581 GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA 5641 CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG 5701 GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT 5761 GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGAAT TCGCTAGAGG GAGGCTAACT 5821 GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT AAAAAGACAG 5881 AATAAAACGC ACGGTGTTGG GTCGTTTGTT CATAAACGCG GGGTTCGGTC CCAGGGCTGG 5941 CACTCTGTCG ATACCCCACC GAGACCCCAT TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT 6001 TCCCCACCCC ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG 6061 GCAGGCCCTG CCATAG //