LOCUS PPOLW.TXT 6101 BP DS-DNA CIRCULAR SYN 26-MAR-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Plasmid from Blueheron biotechnology. Used for cloning codon optimized genes into it. FEATURES Location/Qualifiers promoter 113..310 /note="EF-1alpha promoter core" promoter 113..1291 /note="EF1a promoter and UTR" exon 311..343 /note="EF-1a exon 1" intron 344..1283 /note="EF-1a intron A" exon 1283..1291 /note="EF-1a exon 2 leader" CDS 1353..>2861 /note="HPV1 L1 (prototype, codon modified)" mutation 1703..1703 /note="silent G->T makes Age1 for screening" mutation 1713..1713 /note="Phe->Leu" mutation 2826..2828 /note="this Val is Leu in Meissner(3-3)" mRNA 2918..>3506 /note="WPRE" polyA_signal 3554..>3779 /note="SV40 late polyA" rep_origin 3780..4534 /note="MB1 ori" repeat_region 4709..4772 /note="SV40 21 bp repeats" promoter 4709..4883 /note="SV40 early promoter" rep_origin 4732..4883 /note="SV40 ori" mRNA 4781..4830 /note="SV40 transcription start points" promoter 4918..4973 /note="EM7" CDS 4993..5787 /note="Kan/Neo resistance" polyA_signal 5835..6101 /note="HSV TK pA signal" polyA_site 5893..5911 /note="HSV TK pA site" BASE COUNT 1295 A 1754 C 1675 G 1377 T 0 OTHER ORIGIN - 1 CCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTGCAAAGA TGGATAAAGT TTTAAACAGA 61 GAGGAATCTT TGCAGCTAAT GGACCTTGTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 121 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 181 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 241 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 301 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 361 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 421 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 481 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 541 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 601 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 661 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 721 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 781 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 841 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 901 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 961 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1021 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1081 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1141 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1201 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1261 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA 1321 GATCCATTGT GCTGGATCCA CCGGTCGCCA CCATGGCCGT CTGGCTGCCA GCCCAGAACA 1381 AGTTCTACTT GCCCCCACAA CCAATTACCC GCATTCTGAG TACCGACGAA TACGTCACAC 1441 GCACAAACTT GTTCTATCAC GCCACCAGCG AGAGATTGCT CCTCGTCGGC CACCCCCTGT 1501 TCGAAATCAG TTCCAACCAG ACCGTCACCA TCCCCAAAGT CTCCCCCAAC GCCTTCCGCG 1561 TCTTCCGCGT CAGATTCGCC GACCCCAACC GCTTCGCCTT CGGCGACAAA GCCATCTTCA 1621 ACCCCGAGAC CGAGCGCCTG GTCTGGGGGT TGCGCGGCAT CGAAATCGGC CGCGGGCAAC 1681 CCCTGGGCAT CGGCATCACC GGTCATCCCT TGCTCAACAA ACTGGACGAC GCCGAGAACC 1741 CCACCAACTA CATCAACACC CACGCCAACG GCGACAGCCG CCAGAACACC GCCTTCGACG 1801 CCAAGCAGAC CCAGATGTTC TTGGTCGGAT GCACCCCCGC CAGCGGCGAG CATTGGACCA 1861 GCTCCCGGTG TCCCGGCGAG CAGGTCAAGT TGGGCGATTG TCCACGGGTC CAGATGATCG 1921 AAAGCGTCAT CGAGGACGGC GATATGATGG ACATCGGCTT CGGCGCAATG GACTTCGCCG 1981 CCCTGCAACA GGATAAGAGC GACGTGCCCC TGGACGTCGT CCAGGCCACC TGCAAGTACC 2041 CCGACTACAT TCGCATGAAT CACGAGGCAT ACGGGAATAG CATGTTCTTC TTCGCAAGAA 2101 GGGAACAGAT GTACACACGC CATTTCTTCA CAAGAGGCGG CAGCGTCGGC GACAAAGAAG 2161 CCGTGCCCCA GTCACTCTAC CTGACCGCCG ACGCCGAGCC CCGCACCACC CTGGCCACCA 2221 CCAACTACGT CGGGACCCCC TCCGGGAGCA TGGTCAGCAG CGACGTGCAG CTCTTCAACC 2281 GGAGCTATTG GTTGCAGAGA TGCCAGGGGC AAAACAACGG GATCTGTTGG CGCAATCAGC 2341 TGTTCATCAC CGTCGGCGAC AACACACGCG GCACCTCCCT GAGCATTTCC ATGAAGAACA 2401 ACGCCTCCAC CACCTACTCT AACGCCAACT TCAACGACTT CTTGCGCCAC ACCGAGGAGT 2461 TCGACTTGAG CTTCATCGTC CAATTGTGCA AAGTCAAACT GACCCCTGAG AACTTGGCAT 2521 ACATCCACAC TATGGATCCC AACATCCTGG AAGACTGGCA GTTGAGCGTC AGCCAGCCAC 2581 CCACAAACCC ATTGGAGGAC CAGTACCGCT TCCTGGGCAG CAGTCTCGCC GCTAAGTGCC 2641 CCGAGCAAGC CCCACCCGAA CCACAGACCG ACCCCTACAG CCAGTACAAG TTTTGGGAAG 2701 TGGACTTGAC CGAGCGCATG AGCGAGCAGC TGGATCAGTT CCCATTGGGC CGCAAGTTCT 2761 TGTACCAGTC CGGGATGACC CAGAGGACCG CCACCAGCAG TACAACCAAA AGGAAGACCG 2821 TCAGAGTCAG CACCAGCGCA AAAAGGCGCA GGAAAGCCTG AGGCTCGAGG CTAGTGGATC 2881 CCCCGGGCTG CAGGAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT 2941 TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC 3001 TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT 3061 GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG 3121 CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG 3181 TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC 3241 CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT 3301 GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT 3361 GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG 3421 CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG 3481 GATCTCCCTT TGGGCCGCCT CCCCGCATCG ATACCGTCGG CCGCTTCCCT TTAGTGAGGG 3541 TTAATGCTTC GAGCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA 3601 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC 3661 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT 3721 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGAA 3781 TTCTTAATTA ACTAGCCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 3841 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 3901 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 3961 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC 4021 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 4081 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 4141 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 4201 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 4261 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 4321 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 4381 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 4441 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 4501 GCTGGCCTTT TGCTCACATG TTCTTAATTA AGCTGTACAC TGTGGAATGT GTGTCAGTTA 4561 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 4621 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 4681 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 4741 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGACTGAC TAATTTTTTT TATTTATGCA 4801 GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 4861 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG 4921 CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACTCACTAT 4981 AGGAGGGCCA CCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 5041 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 5101 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 5161 CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 5221 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 5281 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 5341 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 5401 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 5461 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 5521 AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACACATGGCG ATGCCTGCTT GCCGAATATC 5581 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC 5641 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 5701 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 5761 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 5821 CGAATTCGCT AGAGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG 5881 CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGT GTTGGGTCGT TTGTTCATAA 5941 ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC CCACCGAGAC CCCATTGGGG 6001 CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC CCAAGTTCGG GTGAAGGCCC 6061 AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA G //