LOCUS PL2YN.TXT 6609 BP DS-DNA CIRCULAR SYN 26-MAR-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Plasmid from Blueheron biotechnology. Used for cloning codon optimized genes into it. FEATURES Location/Qualifiers promoter 113..310 /note="EF-1alpha promoter core" promoter 113..1291 /note="EF1a promoter and UTR" exon 311..343 /note="EF-1a exon 1" intron 344..1283 /note="EF-1a intron A" exon 1283..1291 /note="EF-1a exon 2 leader" CDS 1396..>2814 /note="HPV16 L2" CDS <2815..>2835 /note="linker" CDS 2836..>3357 /note="EYFP N-terminal fragment" mutation 3028..3028 /note="T->C makes F64L" misc_recomb 3352..>3354 /note="Glu172" mRNA 3426..>4014 /note="WPRE" polyA_signal 4062..>4287 /note="SV40 late polyA" rep_origin 4288..5042 /note="MB1 ori" promoter 5217..5391 /note="SV40 early promoter" repeat_region 5217..5280 /note="SV40 21 bp repeats" rep_origin 5240..5391 /note="SV40 ori" mRNA 5289..5338 /note="SV40 transcription start points" promoter 5426..5481 /note="EM7" CDS 5501..6295 /note="Kan/Neo resistance" polyA_signal 6343..6609 /note="HSV TK pA signal" polyA_site 6401..6419 /note="HSV TK pA site" BASE COUNT 1392 A 2016 C 1823 G 1378 T 0 OTHER ORIGIN - 1 CCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTGCAAAGA TGGATAAAGT TTTAAACAGA 61 GAGGAATCTT TGCAGCTAAT GGACCTTGTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 121 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 181 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 241 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 301 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 361 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 421 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 481 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 541 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 601 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 661 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 721 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 781 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 841 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 901 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 961 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1021 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1081 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1141 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1201 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1261 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA 1321 GATCCATCCT GTACGGAAGT GTTACTTCTG CTCTAAAAGC TGCGGAATTG TACCCGCGGC 1381 CGCTCTAGAG CCACCATGAG GCACAAGAGG AGCGCCAAGA GGACCAAGAG GGCCAGCGCC 1441 ACCCAGCTGT ACAAGACCTG CAAGCAGGCC GGCACCTGCC CCCCCGACAT CATCCCCAAG 1501 GTGGAGGGCA AGACCATCGC CGACCAGATC CTGCAGTACG GCAGCATGGG CGTGTTCTTC 1561 GGCGGCCTGG GCATCGGCAC CGGCAGCGGC ACCGGCGGCA GGACCGGCTA CATCCCCCTG 1621 GGCACCAGGC CCCCCACCGC CACCGACACC CTGGCCCCCG TGAGGCCCCC CCTGACCGTG 1681 GACCCCGTGG GCCCCAGCGA CCCCAGCATC GTGAGCCTGG TGGAGGAGAC CAGCTTCATC 1741 GACGCCGGCG CCCCCACCAG CGTGCCCAGC ATCCCCCCCG ACGTGAGCGG CTTCAGCATC 1801 ACCACCAGCA CCGACACCAC CCCCGCCATC CTGGACATCA ACAACACCGT GACCACCGTG 1861 ACCACCCACA ACAACCCCAC CTTCACCGAC CCCAGCGTGC TGCAGCCCCC CACCCCCGCC 1921 GAGACCGGCG GCCACTTCAC CCTGAGCAGC AGCACCATCA GCACCCACAA CTACGAGGAG 1981 ATCCCCATGG ACACCTTCAT CGTGAGCACC AACCCCAACA CCGTGACCAG CAGCACCCCC 2041 ATCCCCGGCA GCAGGCCCGT GGCCAGGCTG GGCCTGTACA GCAGGACCAC CCAGCAGGTG 2101 AAGGTGGTGG ACCCCGCCTT CGTGACCACC CCCACCAAGC TGATCACCTA CGACAACCCC 2161 GCCTACGAGG GCATCGACGT GGACAACACC CTGTACTTCA GCAGCAACGA CAACAGCATC 2221 AACATCGCCC CCGACCCCGA CTTCCTGGAC ATCGTGGCCC TGCACAGGCC CGCCCTGACC 2281 AGCAGGAGGA CCGGCATCAG GTACAGCAGG ATCGGCAACA AGCAGACCCT GAGGACCAGG 2341 AGCGGCAAGA GCATCGGCGC CAAGGTGCAC TACTACTACG ACCTGAGCAC CATCGACCCC 2401 GCCGAGGAGA TCGAGCTGCA GACCATCACC CCCAGCACCT ACACCACCAC CAGCCACGCC 2461 GCCAGCCCCA CCAGCATCAA CAACGGCCTG TACGACATCT ACGCCGACGA CTTCATCACC 2521 GACACCAGCA CCACCCCCGT GCCCAGCGTG CCCAGCACCA GCCTGAGCGG CTACATCCCC 2581 GCCAACACCA CCATCCCCTT CGGTGGCGCC TACAACATCC CCCTGGTGAG CGGCCCCGAC 2641 ATCCCCATCA ACATCACCGA CCAGGCCCCC AGCCTGATCC CCATCGTGCC CGGCAGCCCC 2701 CAGTACACCA TCATCGCCGA CGCCGGCGAC TTCTACCTGC ACCCCAGCTA CTACATGCTG 2761 AGGAAGAGGA GGAAGAGGCT GCCCTACTTC TTCAGCGACG TGAGCCTGGC CGCGCTAGGC 2821 GGAACCGGGA GCGGGATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC 2881 CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG 2941 GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC 3001 GTGCCCTGGC CCACCCTCGT GACCACCCTC GGCTACGGCC TGCAGTGCTT CGCCCGCTAC 3061 CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG 3121 GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC 3181 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC 3241 AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC 3301 GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGTGAGTC 3361 CGGACTCAGA TCTCGACACT AGTGGATCCC CCGGGCTGCA GGAATTCGAT ATCAAGCTTA 3421 TCGATAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG 3481 TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT 3541 CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG 3601 AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC 3661 CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC 3721 TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC 3781 GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC 3841 TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG 3901 CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC 3961 GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCATCGAT 4021 ACCGTCGGCC GCTTCCCTTT AGTGAGGGTT AATGCTTCGA GCAGACATGA TAAGATACAT 4081 TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT 4141 TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA 4201 CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA 4261 GTAAAACCTC TACAAATGTG GTATGGAATT CTTAATTAAC TAGCCATGAC CAAAATCCCT 4321 TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT 4381 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA 4441 GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC 4501 AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC 4561 AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT 4621 GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG 4681 GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC 4741 TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG 4801 AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG 4861 CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT 4921 GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC 4981 GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTAATTAAG 5041 CTGTACACTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG 5101 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG 5161 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC 5221 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA 5281 TGACTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT 5341 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC 5401 TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT 5461 ATCGGCATAG TATAATACGA CTCACTATAG GAGGGCCACC ATGATTGAAC AAGATGGATT 5521 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA 5581 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT 5641 TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAAGACGAGG CAGCGCGGCT 5701 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC 5761 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT 5821 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA 5881 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG 5941 GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC 6001 AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC GGCGAGGATC TCGTCGTGAC 6061 ACATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT 6121 CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA 6181 TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC 6241 CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG 6301 ACTCTGGGGT TCGAAATGAC CGACCAAGCG AATTCGCTAG AGGGAGGCTA ACTGAAACAC 6361 GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA 6421 CGCACGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG 6481 TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC 6541 CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC 6601 CTGCCATAG //