LOCUS PL2YC.TXT 6288 BP DS-DNA CIRCULAR SYN 26-MAR-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Plasmid from Blueheron biotechnology. Used for cloning codon optimized genes into it. FEATURES Location/Qualifiers promoter 113..310 /note="EF-1alpha promoter core" promoter 113..1291 /note="EF1a promoter and UTR" exon 311..343 /note="EF-1a exon 1" intron 344..1283 /note="EF-1a intron A" exon 1283..1291 /note="EF-1a exon 2 leader" CDS 1396..>2814 /note="HPV16 L2" CDS <2815..>2835 /note="linker" CDS <2836..3036 /note="EYFP C-terminal fragment" mRNA 3105..>3693 /note="WPRE" polyA_signal 3741..>3966 /note="SV40 late polyA" rep_origin 3967..4721 /note="MB1 ori" promoter 4896..5070 /note="SV40 early promoter" repeat_region 4896..4959 /note="SV40 21 bp repeats" rep_origin 4919..5070 /note="SV40 ori" mRNA 4968..5017 /note="SV40 transcription start points" promoter 5105..5160 /note="EM7" CDS 5180..5974 /note="Kan/Neo resistance" polyA_signal 6022..6288 /note="HSV TK pA signal" polyA_site 6080..6098 /note="HSV TK pA site" BASE COUNT 1310 A 1923 C 1728 G 1327 T 0 OTHER ORIGIN - 1 CCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTGCAAAGA TGGATAAAGT TTTAAACAGA 61 GAGGAATCTT TGCAGCTAAT GGACCTTGTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 121 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 181 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 241 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 301 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 361 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 421 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 481 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 541 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 601 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 661 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 721 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 781 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 841 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 901 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 961 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1021 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1081 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1141 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1201 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1261 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA 1321 GATCCATCCT GTACGGAAGT GTTACTTCTG CTCTAAAAGC TGCGGAATTG TACCCGCGGC 1381 CGCTCTAGAG CCACCATGAG GCACAAGAGG AGCGCCAAGA GGACCAAGAG GGCCAGCGCC 1441 ACCCAGCTGT ACAAGACCTG CAAGCAGGCC GGCACCTGCC CCCCCGACAT CATCCCCAAG 1501 GTGGAGGGCA AGACCATCGC CGACCAGATC CTGCAGTACG GCAGCATGGG CGTGTTCTTC 1561 GGCGGCCTGG GCATCGGCAC CGGCAGCGGC ACCGGCGGCA GGACCGGCTA CATCCCCCTG 1621 GGCACCAGGC CCCCCACCGC CACCGACACC CTGGCCCCCG TGAGGCCCCC CCTGACCGTG 1681 GACCCCGTGG GCCCCAGCGA CCCCAGCATC GTGAGCCTGG TGGAGGAGAC CAGCTTCATC 1741 GACGCCGGCG CCCCCACCAG CGTGCCCAGC ATCCCCCCCG ACGTGAGCGG CTTCAGCATC 1801 ACCACCAGCA CCGACACCAC CCCCGCCATC CTGGACATCA ACAACACCGT GACCACCGTG 1861 ACCACCCACA ACAACCCCAC CTTCACCGAC CCCAGCGTGC TGCAGCCCCC CACCCCCGCC 1921 GAGACCGGCG GCCACTTCAC CCTGAGCAGC AGCACCATCA GCACCCACAA CTACGAGGAG 1981 ATCCCCATGG ACACCTTCAT CGTGAGCACC AACCCCAACA CCGTGACCAG CAGCACCCCC 2041 ATCCCCGGCA GCAGGCCCGT GGCCAGGCTG GGCCTGTACA GCAGGACCAC CCAGCAGGTG 2101 AAGGTGGTGG ACCCCGCCTT CGTGACCACC CCCACCAAGC TGATCACCTA CGACAACCCC 2161 GCCTACGAGG GCATCGACGT GGACAACACC CTGTACTTCA GCAGCAACGA CAACAGCATC 2221 AACATCGCCC CCGACCCCGA CTTCCTGGAC ATCGTGGCCC TGCACAGGCC CGCCCTGACC 2281 AGCAGGAGGA CCGGCATCAG GTACAGCAGG ATCGGCAACA AGCAGACCCT GAGGACCAGG 2341 AGCGGCAAGA GCATCGGCGC CAAGGTGCAC TACTACTACG ACCTGAGCAC CATCGACCCC 2401 GCCGAGGAGA TCGAGCTGCA GACCATCACC CCCAGCACCT ACACCACCAC CAGCCACGCC 2461 GCCAGCCCCA CCAGCATCAA CAACGGCCTG TACGACATCT ACGCCGACGA CTTCATCACC 2521 GACACCAGCA CCACCCCCGT GCCCAGCGTG CCCAGCACCA GCCTGAGCGG CTACATCCCC 2581 GCCAACACCA CCATCCCCTT CGGTGGCGCC TACAACATCC CCCTGGTGAG CGGCCCCGAC 2641 ATCCCCATCA ACATCACCGA CCAGGCCCCC AGCCTGATCC CCATCGTGCC CGGCAGCCCC 2701 CAGTACACCA TCATCGCCGA CGCCGGCGAC TTCTACCTGC ACCCCAGCTA CTACATGCTG 2761 AGGAAGAGGA GGAAGAGGCT GCCCTACTTC TTCAGCGACG TGAGCCTGGC CGCGCTAGGC 2821 GGAACCGGGA GCGGGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC 2881 ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCTACCA GTCCGCCCTG 2941 AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC 3001 GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTGAGTCC GGACTCAGAT CTCGACACTA 3061 GTGGATCCCC CGGGCTGCAG GAATTCGATA TCAAGCTTAT CGATAATCAA CCTCTGGATT 3121 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG 3181 GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT 3241 CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC 3301 AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA 3361 CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC 3421 TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT 3481 CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT 3541 GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC 3601 CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA 3661 CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA CCGTCGGCCG CTTCCCTTTA 3721 GTGAGGGTTA ATGCTTCGAG CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC 3781 AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT 3841 TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT 3901 TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG 3961 TATGGAATTC TTAATTAACT AGCCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC 4021 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC 4081 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT 4141 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT 4201 ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 4261 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 4321 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG 4381 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 4441 CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG 4501 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 4561 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 4621 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 4681 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTAATTAAGC TGTACACTGT GGAATGTGTG 4741 TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA 4801 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 4861 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC 4921 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GACTGACTAA TTTTTTTTAT 4981 TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT 5041 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC 5101 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC 5161 TCACTATAGG AGGGCCACCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC 5221 TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC 5281 CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC 5341 CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG 5401 CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT 5461 GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC 5521 CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA 5581 CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA 5641 TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT 5701 CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACA CATGGCGATG CCTGCTTGCC 5761 GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT 5821 GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG 5881 CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT 5941 CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC 6001 GACCAAGCGA ATTCGCTAGA GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG 6061 GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGTGTT GGGTCGTTTG 6121 TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC 6181 ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG 6241 AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAG //