LOCUS PCRPVL1.TX 6108 BP DS-DNA CIRCULAR SYN 26-MAR-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Plasmid from Blueheron biotechnology. Used for cloning codon optimized genes into it. FEATURES Location/Qualifiers promoter 113..310 /note="EF-1alpha promoter core" promoter 113..1291 /note="EF1a promoter and UTR" exon 311..343 /note="EF-1a exon 1" intron 344..1283 /note="EF-1a intron A" exon 1283..1291 /note="EF-1a exon 2 leader" CDS 1353..2870 /note="codon modified CRPV L1" mRNA 2925..>3513 /note="WPRE" polyA_signal 3561..>3786 /note="SV40 late polyA" rep_origin 3787..4541 /note="MB1 ori" promoter 4716..4890 /note="SV40 early promoter" repeat_region 4716..4779 /note="SV40 21 bp repeats" rep_origin 4739..4890 /note="SV40 ori" mRNA 4788..4837 /note="SV40 transcription start points" promoter 4925..4980 /note="EM7" CDS 5000..5794 /note="Kan/Neo resistance" polyA_signal 5842..6108 /note="HSV TK pA signal" polyA_site 5900..5918 /note="HSV TK pA site" BASE COUNT 1349 A 1701 C 1645 G 1413 T 0 OTHER ORIGIN - 1 CCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTGCAAAGA TGGATAAAGT TTTAAACAGA 61 GAGGAATCTT TGCAGCTAAT GGACCTTGTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 121 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 181 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 241 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 301 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 361 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 421 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 481 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 541 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 601 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 661 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 721 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 781 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 841 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 901 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 961 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1021 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1081 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1141 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1201 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1261 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA 1321 GATCCATTGT GCTGGATCCA CCGGTCGCCA CCATGGCCGT CTGGCTCAGC ACCCAAAACA 1381 AATTCTATCT CCCCCCCCAA CCCGTTACCA AAATCCCCTC AACCGACGAA TATGTCACCA 1441 GAACCAATGT GTTCTACTAC GCCAGTTCCG ATAGATTGTT GACCGTCGGC CACCCCTATT 1501 ACGAGATCAG GGACAAGGGG ACAATGTTGG TCCCCAAAGT CAGCCCCAAT CAGTATCGCG 1561 TGTTCCGCAT TAAGTTGCCC GATCCCAACA AATTCGCCTT CGGCGATAAA CAGCTCTACG 1621 ACCCCGAAAA AGAGAGATTG GTTTGGTGTT TGCGCGGCAT CGAAGTTAAC AGGGGGCAAC 1681 CCTTGGGCGT GTCCGTCACC GGCAATCCCA TTTTCAACAA GTTCGACGAC GTGGAAAACC 1741 CAACCAAATA CTATAACAAT CACGCCGATC AACAGGATTA TCGCAAGTCA ATGGCCTTTG 1801 ATCCAAAACA AGTCCAACTC CTGATGTTGG GCTGCGTGCC CGCAACCGGC GAGCATTGGG 1861 CCCAAGCCAA ACAATGCGCC GAAGACCCCC CCCAGCAAAC AGATTGCCCA CCAATCGAGT 1921 TGGTCAATAC CGTCATCGAG GACGGCGATA TGTGCGAGAT CGGGTTCGGC GCTATGGATC 1981 ACAAGACCCT CCAAGCATCC CTGAGCGAAG TCCCATTGGA ACTGGCCCAA AGCATTTCAA 2041 AGTACCCCGA TTACTTGAAG ATGCAGAAGG ACCAATTCGG CGACAGCATG TTCTTCTACG 2101 CCCGCCGCGA ACAGATGTAC GCCCGCCACT TCTTTTCACG CGCCGGCGGC GATAAAGAGA 2161 ACGTCAAATC ACGCGCATAC ATCAAGAGGA CCCAAATGCA AGGCGAAGCC AACGCAAACA 2221 TCGCCACCGA TAACTATTGT ATTACCCCCT CCGGGAGCCT CGTCAGCTCA GACAGCCAAG 2281 TCTTCAATAG AGCCTACTGG TTGCAAAAGG CCCAGGGGAT GAATAACGGC GTCTGTTGGG 2341 ATAACCAGAT CTTCGTCACC GTCGTGGACA ATACACGCGG CACCATCCTG TCCTTGGTTA 2401 CCAAGAGCAA AGACGCCAAC CAGGAGGATC CCTGGAAGAA TTCCACCTTC AGCAGTTACT 2461 TGCGCCACGT CGAAGAATAC GAGCTCCAGT TCGTCTTGCA ATTGTGCAAA GTCAAACTGA 2521 CCCCAGAGAA TTTGAGCTAT TTGCACTCAA TGCATCCCAC CATTATCGAC AACTGGCAGC 2581 TCAGCGTCAG CGCCCAACCA TCCGGCACCT TGGAAGATCA ATATCGCTAT CTGCAAAGCA 2641 TCGCCACAAA GTGCCCCCCA CCCGAGCCAC CAAAGGAGAA TACCGATCCC TACAAGAATT 2701 ACAAATTCTG GGAAGTGGAC CTGAGCGAAA AGTTGAGCGA CCAATTGGAC CAATACCCCT 2761 TGGGGCGCAA ATTCTTGAAC CAGTCCGGGC TCCAGCGCAT CGGCACCAAG CGCCCCGCCC 2821 CCGCCCCCGT CTCCATCGTC AAGAGCAGCA AGAGGAAACG CAGGACCTGA GCTCGAGGCT 2881 AGGGATCCCC CGGGCTGCAG GAATTCGATA TCAAGCTTAT CGATAATCAA CCTCTGGATT 2941 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG 3001 GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT 3061 CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC 3121 AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA 3181 CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC 3241 TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT 3301 CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT 3361 GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC 3421 CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA 3481 CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA CCGTCGGCCG CTTCCCTTTA 3541 GTGAGGGTTA ATGCTTCGAG CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC 3601 AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT 3661 TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT 3721 TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG 3781 TATGGAATTC TTAATTAACT AGCCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC 3841 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC 3901 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT 3961 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT 4021 ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 4081 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 4141 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG 4201 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 4261 CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG 4321 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 4381 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 4441 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 4501 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTAATTAAGC TGTACACTGT GGAATGTGTG 4561 TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA 4621 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 4681 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC 4741 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GACTGACTAA TTTTTTTTAT 4801 TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT 4861 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC 4921 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC 4981 TCACTATAGG AGGGCCACCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC 5041 TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC 5101 CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC 5161 CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG 5221 CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT 5281 GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC 5341 CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA 5401 CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA 5461 TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT 5521 CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACA CATGGCGATG CCTGCTTGCC 5581 GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT 5641 GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG 5701 CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT 5761 CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC 5821 GACCAAGCGA ATTCGCTAGA GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG 5881 GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGTGTT GGGTCGTTTG 5941 TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC 6001 ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG 6061 AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAG //