LOCUS PCMMF.GB APR 6777 BP DS-DNA CIRCULAR SYN 07-APR-2002 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers enhancer 1..659 /note="CMV enhancer" misc_feature 669..750 /note="CMV promoter core" intron 890..1022 /note="intron" intron 989..994 /note="lariat branch-point" misc_feature 1067..1085 /note="T7 promoter" CDS 1144..2508 /note="M-M2" mutation <2515..2519 /note="insert TATAA" misc_feature complement(2549..2567) /note="T3 promoter" polyA_signal 2576..2797 /note="SV40 late polyA" rep_origin 2892..3347 /note="F1 Ori" promoter <3409..>3827 /note="SV40 promoter" rep_origin 3672..>3807 /note="SV40 Ori" misc_feature 3728..3734 /note="SV40 promoter core" mutation 3741..3741 /note="G->A kills SELP ATG" CDS 3852..4571 /note="EGFP" polyA_signal 4626..4674 /note="synthetic polyA" CDS 5085..5945 /note="AmpR" rep_origin 6094..6764 /note="Ori" BASE COUNT 1740 A 1761 C 1654 G 1622 T 0 OTHER ORIGIN - 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA ATATTGGCTA 61 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC 121 AATATGACCG CCATGTTGGC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG 181 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC 241 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC 361 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA 421 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG 481 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC 541 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTG 661 CGATCGCCCG CCCCGTTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 721 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC ACTAGAAGCT TTATTGCGGT AGTTTATCAC 781 AGTTAAATTG CTAACGCAGT CAGTGCTTCT GACACAACAG TCTCGAACTT AAGCTGCAGT 841 GACTCTCTTA AGGTAGCCTT GCAGAAGTTG GTCGTGAGGC ACTGGGCAGG TAAGTATCAA 901 GGTTACAAGA CAGGTTTAAG GAGACCAATA GAAACTGGGC TTGTCGAGAC AGAGAAGACT 961 CTTGCGTTTC TGATAGGCAC CTATTGGTCT TACTGACATC CACTTTGCCT TTCTCTCCAC 1021 AGGTGTCCAC TCCCAGTTCA ATTACAGCTC TTAAGGCTAG AGTACTTAAT ACGACTCACT 1081 ATAGGCTAGC CTCGAGAATT CACGCGTTAC GTATCGGATC CAGAATTCGT GATTATACCC 1141 GGGATGGAGA CCTACGTGAA TAAGCTGCAC GAGGGAAGCA CCTACACCGC CGCTGTGCAG 1201 TACAATGTGC TGGAGAAGGA CGATGATCCT GCTTCCCTGA CCATCTGGGT GCCCATGTTT 1261 CAGTCTAGCA TGCCCGCCGA TCTGCTGATT AAGGAGCTGG CCAACGTGAA CATCCTGGTG 1321 AAGCAGATCA GCACCCCAAA GGGACCTTCC CTGAGAGTGA TGATTAACTC CAGAAGCGCC 1381 GTGCTGGCCC AGATGCCCTC TAAGTTCACA ATCTGCGCTA ATGTGTCCCT GGACGAGAGA 1441 TCCAAGCTGG CTTACGATGT GACCACCCCA TGCGAGATCA AGGCTTGTTC TCTGACCTGT 1501 CTGAAGTCCA AGAATATGCT GACCACCGTG AAGGACCTGA CAATGAAAAC ACTGAATCCC 1561 ACCCACGATA TCATCGCCCT GTGTGAGTTT GAGAATATCG TGACAAGCAA GAAGGTCATC 1621 ATCCCAACAT ACCTGAGATC TATCTCTGTG AGGAATAAGG ATCTGAACAC ACTGGAGAAT 1681 ATCACAACCA CCGAGTTTAA GAACGCTATC ACAAACGCCA AGATCATCCC TTACAGCGGA 1741 CTGCTGCTGG TCATCACAGT GACCGATAAC AAGGGCGCCT TCAAGTACAT CAAGCCACAG 1801 TCCCAGTTCA TCGTGGATCT GGGCGCTTAC CTGGAGAAGG AGAGCATCTA CTACGTGACC 1861 ACCAACTGGA AGCACACAGC TACAAGATTC GCCATCAAGC CCATGGAGGA CCCTGATCAG 1921 GCTATGTCTA GGCGCAACCC TTGCAAGTTT GAGATCCGGG GACACTGTCT GAACGGCAAG 1981 CGGTGTCACT TTTCTCACAA TTACTTTGAG TGGCCTCCTC ACGCCCTGCT GGTGCGGCAG 2041 AACTTTATGC TGAATAGAAT CCTGAAGTCT ATGGACAAGT CTATCGATAC CCTGTCCGAG 2101 ATCTCCGGAG CCGCTGAGCT GGACAGAACC GAGGAGTACG CTCTGGGCGT GGTGGGCGTG 2161 CTGGAGTCTT ACATCGGCAG CATCAACAAT ATCACAAAGC AGTCCGCTTG TGTGGCCATG 2221 TCTAAGCTGC TGACAGAGCT GAACTCTGAC GATATCAAGA AGCTGCGGGA TAACGAGGAG 2281 CTGAATTCCC CTAAGATCCG CGTGTACAAC ACCGTGATCT CCTACATCGA GTCCAACCGC 2341 AAGAATAATA AGCAGACAAT CCACCTGCTG AAGCGGCTGC CTGCCGACGT GCTGAAGAAA 2401 ACAATCAAGA ACACCCTGGA TATCCACAAG AGCATCACCA TCAATAACCC CAAGGAGTCT 2461 ACCGTGTCCG ACACAAACGA TCACGCCAAG AACAACGACA CAACATGACC CGGGTATAAT 2521 CTGAATTCGT CGACCCGGGC GGCCGCTTCC CTTTAGTGAG GGTTAATGCT TCGAGCAGAC 2581 ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC 2641 TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA 2701 CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG 2761 GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCCGATAAGG ATCGATCCGG 2821 GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA 2881 ATGGCGAATG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG 2941 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC 3001 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG 3061 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC 3121 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT 3181 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC 3241 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA 3301 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTCCT GATGCGGTAT 3361 TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAC GCGGATCTGC GCAGCACCAT 3421 GGCCTGAAAT AACCTCTGAA AGAGGAACTT GGTTAGGTAC CTTCTGAGGC GGAAAGAACC 3481 AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 3541 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC 3601 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC 3661 TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT 3721 GACTAATTTT TTTTATTTAT ACAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA 3781 AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTTGAT TGGGATCCAC 3841 CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG 3901 TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG 3961 ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC 4021 CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG 4081 ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC 4141 GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG 4201 GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA 4261 TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA 4321 AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG 4381 TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 4441 CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG 4501 ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC 4561 TGTACAAGTA AAGCGGCCCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGATG 4621 GCCGCAATAA AATATCTTTA TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAATCGA 4681 TAGCGATAAG GATCCGCGTA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT 4741 TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC 4801 CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT 4861 CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG 4921 TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC 4981 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC 5041 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT 5101 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG 5161 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG 5221 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA 5281 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC 5341 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG 5401 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA 5461 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC 5521 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG 5581 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA 5641 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA 5701 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG 5761 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG 5821 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG 5881 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT 5941 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT 6001 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC 6061 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 6121 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 6181 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 6241 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 6301 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 6361 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 6421 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 6481 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 6541 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 6601 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 6661 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 6721 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGGCTCGA CAGATCT //