LOCUS PCLUCF.GB APR 7053 BP DS-DNA CIRCULAR SYN 07-APR-2002 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers enhancer 1..659 /note="CMV enhancer" misc_feature 669..750 /note="CMV promoter core" intron 890..1022 /note="intron" intron 989..994 /note="lariat branch-point" misc_feature 1067..1085 /note="T7 promoter" misc_feature complement(2825..2843) /note="T3 promoter" polyA_signal 2852..3073 /note="SV40 late polyA" rep_origin 3168..3623 /note="F1 Ori" promoter 3685..>3731 /note="SV40 Promoter" polyA_signal 4902..4950 /note="synthetic polyA" CDS 5361..6221 /note="AmpR" rep_origin 6370..7040 /note="Ori" frag 3732..4854 /note="48 to 1170 of p∆SELP" promoter <3732..>4103 /note="SV40 promoter [Split]" misc_feature complement(<3732..3767) /note="SV40 minor late 19S RNA [Split]" intron complement(<3732..3736) /note="SV40 minor late 19S RNA intron [Split]" misc_feature 3759..3759 /note="begin homology to pCDNA3.1 SV40" enhancer 3781..3923 /note="SV40 enhancer elements" misc_feature 3781..3781 /note="begin homology to EGFP-N1 SV40" misc_feature 3928..3991 /note="SV40 21bp repeats" rep_origin 3948..>4083 /note="SV40 Ori" mRNA 3997..4003 /note="early-late transcription startpoints" misc_feature 4004..4010 /note="SV40 promoter core" CDS 4015..>4086 /note="SELP protein (ATG dead)" mutation 4017..4017 /note="G->A kills SELP ATG" mRNA 4037..4043 /note="early-early transcription start points" CDS 4128..4847 /note="EGFP" frag 1096..2815 /note="2645 to 4364 of pLucf" mutation 1103..1103 /note="G->C discovered by sequencing" CDS 1141..2793 /note="Luc2 (codmod, weighs 60.6kD)" BASE COUNT 1746 A 1878 C 1784 G 1645 T 0 OTHER ORIGIN - 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA ATATTGGCTA 61 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC 121 AATATGACCG CCATGTTGGC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG 181 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC 241 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC 361 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA 421 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG 481 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC 541 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTG 661 CGATCGCCCG CCCCGTTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 721 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC ACTAGAAGCT TTATTGCGGT AGTTTATCAC 781 AGTTAAATTG CTAACGCAGT CAGTGCTTCT GACACAACAG TCTCGAACTT AAGCTGCAGT 841 GACTCTCTTA AGGTAGCCTT GCAGAAGTTG GTCGTGAGGC ACTGGGCAGG TAAGTATCAA 901 GGTTACAAGA CAGGTTTAAG GAGACCAATA GAAACTGGGC TTGTCGAGAC AGAGAAGACT 961 CTTGCGTTTC TGATAGGCAC CTATTGGTCT TACTGACATC CACTTTGCCT TTCTCTCCAC 1021 AGGTGTCCAC TCCCAGTTCA ATTACAGCTC TTAAGGCTAG AGTACTTAAT ACGACTCACT 1081 ATAGGCTAGC CTCGAGAATT CTCTAGAGCT TGGCAATCCG GTACTGTTGG TAAAGCCACC 1141 ATGGAAGATG CCAAAAACAT TAAGAAGGGC CCAGCGCCAT TCTACCCACT CGAAGACGGG 1201 ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG CCCTGGTGCC CGGCACCATC 1261 GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG CCGAGTACTT CGAGATGAGC 1321 GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT GGGCTGAATA CAAACCATCG GATCGTGGTG 1381 TGCAGCGAGA ATAGCTTGCA GTTCTTCATG CCCGTGTTGG GTGCCCTGTT CATCGGTGTG 1441 GCTGTGGCCC CAGCTAACGA CATCTACAAC GAGCGCGAGC TGCTGAACAG CATGGGCATC 1501 AGCCAGCCCA CCGTCGTATT CGTGAGCAAG AAAGGGCTGC AAAAGATCCT CAACGTGCAA 1561 AAGAAGCTAC CGATCATACA AAAGATCATC ATCATGGATA GCAAGACCGA CTACCAGGGC 1621 TTCCAAAGCA TGTACACCTT CGTGACTTCC CATTTGCCAC CCGGCTTCAA CGAGTACGAC 1681 TTCGTGCCCG AGAGCTTCGA CCGGGACAAA ACCATCGCCC TGATCATGAA CAGTAGTGGC 1741 AGTACCGGAT TGCCCAAGGG CGTAGCCCTA CCGCACCGCA CCGCTTGTGT CCGATTCAGT 1801 CATGCCCGCG ACCCCATCTT CGGCAACCAG ATCATCCCCG ACACCGCTAT CCTCAGCGTG 1861 GTGCCATTTC ACCACGGCTT CGGCATGTTC ACCACGCTGG GCTACTTGAT CTGCGGCTTT 1921 CGGGTCGTGC TCATGTACCG CTTCGAGGAG GAGCTATTCT TGCGCAGCTT GCAAGACTAT 1981 AAGATTCAAT CTGCCCTGCT GGTGCCCACA CTATTTAGCT TCTTCGCTAA GAGCACTCTC 2041 ATCGACAAGT ACGACCTAAG CAACTTGCAC GAGATCGCCA GCGGCGGGGC GCCGCTCAGC 2101 AAGGAGGTAG GTGAGGCCGT GGCCAAACGC TTCCACCTAC CAGGCATCCG CCAGGGCTAC 2161 GGCCTGACAG AAACAACCAG CGCCATTCTG ATCACCCCCG AAGGGGACGA CAAGCCTGGC 2221 GCAGTAGGCA AGGTGGTGCC CTTCTTCGAG GCTAAGGTGG TGGACTTGGA CACCGGTAAG 2281 ACACTGGGTG TGAACCAGCG CGGCGAGCTG TGCGTCCGTG GCCCCATGAT CATGAGCGGC 2341 TACGTTAACA ACCCCGAGGC TACAAACGCT CTCATCGACA AGGACGGCTG GCTGCACAGC 2401 GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA TCGTGGACCG GCTGAAGAGC 2461 CTGATCAAAT ACAAGGGCTA CCAGGTAGCC CCAGCCGAAC TGGAGAGCAT CCTGCTGCAA 2521 CACCCCAACA TCTTCGACGC CGGGGTCGCC GGCCTGCCCG ACGACGATGC CGGCGAGCTG 2581 CCCGCCGCAG TCGTCGTGCT GGAACACGGT AAAACCATGA CCGAGAAGGA GATCGTGGAC 2641 TATGTGGCCA GCCAGGTTAC AACCGCCAAG AAGCTGCGCG GTGGTGTTGT GTTCGTGGAC 2701 GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA AGATCCGCGA GATTCTCATT 2761 AAGGCCAAGA AGGGCGGCAA GATCGCCGTG TAATAATTCT AGTGGATCCC CCGGGCGGCC 2821 GCTTCCCTTT AGTGAGGGTT AATGCTTCGA GCAGACATGA TAAGATACAT TGATGAGTTT 2881 GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT 2941 ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT 3001 CATTTTATGT TTCAGGTTCA GGGGGAGATG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC 3061 TACAAATGTG GTAAAATCCG ATAAGGATCG ATCCGGGCTG GCGTAATAGC GAAGAGGCCC 3121 GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGACG CGCCCTGTAG 3181 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG 3241 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT 3301 TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA 3361 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA 3421 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA 3481 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC 3541 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA 3601 CAAAATATTA ACGCTTACAA TTTCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT 3661 ATTTCACACC GCATACGCGG ATCTGCGCAG CACCATGGCC TGAAATAACC TCTGAAAGAG 3721 GAACTTGGTT AGGTACCTTC TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG 3781 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT 3841 AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 3901 TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA 3961 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATACAG 4021 AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG 4081 GCCTAGGCTT TTGCAAAAAG CTTGATTGGG ATCCACCGGT CGCCACCATG GTGAGCAAGG 4141 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG 4201 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC 4261 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC 4321 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT 4381 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG 4441 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG 4501 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA 4561 ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA 4621 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC 4681 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC 4741 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG 4801 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC GGCCCGAAAT 4861 GACCGACCAA GCGACGCCCA ACCTGCCATC ACGATGGCCG CAATAAAATA TCTTTATTTT 4921 CATTACATCT GTGTGTTGGT TTTTTGTGTG AATCGATAGC GATAAGGATC CGCGTATGGT 4981 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA 5041 CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG 5101 TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA 5161 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 5221 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 5281 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 5341 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 5401 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 5461 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 5521 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 5581 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 5641 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 5701 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 5761 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 5821 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 5881 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 5941 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 6001 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 6061 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 6121 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 6181 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 6241 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 6301 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 6361 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 6421 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 6481 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC 6541 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 6601 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 6661 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 6721 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 6781 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 6841 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 6901 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 6961 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7021 GCTGGCCTTT TGCTCACATG GCTCGACAGA TCT //