LOCUS P6L2W.TXT 5932 BP DS-DNA CIRCULAR SYN 26-MAR-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Plasmid from Blueheron biotechnology. Used for cloning codon optimized genes into it. FEATURES Location/Qualifiers promoter 113..310 /note="EF-1alpha promoter core" promoter 113..1291 /note="EF1a promoter and UTR" exon 311..343 /note="EF-1a exon 1" intron 344..1283 /note="EF-1a intron A" exon 1283..1291 /note="EF-1a exon 2 leader" CDS <1353..2732 /note="HPV6a L2 (codon modified)" mRNA 2749..>3337 /note="WPRE" polyA_signal 3385..>3610 /note="SV40 late polyA" rep_origin 3611..4365 /note="MB1 ori" repeat_region 4540..4603 /note="SV40 21 bp repeats" promoter 4540..4714 /note="SV40 early promoter" rep_origin 4563..4714 /note="SV40 ori" mRNA 4612..4661 /note="SV40 transcription start points" promoter 4749..4804 /note="EM7" CDS 4824..5618 /note="Kan/Neo resistance" polyA_signal 5666..5932 /note="HSV TK pA signal" polyA_site 5724..5742 /note="HSV TK pA site" BASE COUNT 1205 A 1807 C 1602 G 1318 T 0 OTHER ORIGIN - 1 CCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTGCAAAGA TGGATAAAGT TTTAAACAGA 61 GAGGAATCTT TGCAGCTAAT GGACCTTGTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 121 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 181 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 241 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 301 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 361 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 421 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 481 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 541 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 601 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 661 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 721 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 781 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 841 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 901 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 961 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1021 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1081 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1141 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1201 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1261 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA 1321 GATCCATTGT GCTGGATCCA CCGGTCGCCA CCATGGCCCA CTCCCGCGCA CGCCGCAGGA 1381 AACGCGCCAG CGCCACCCAA CTGTACCAGA CCTGCAAGTT GACCGGCACC TGTCCACCCG 1441 ACGTCATCCC CAAGGTCGAA CACAACACAA TCGCCGACCA GATCCTGAAG TGGGGCTCCC 1501 TCGGCGTGTT CTTCGGCGGC CTCGGCATCG GGACAGGCAG TGGGACCGGC GGCAGGACCG 1561 GCTACGTCCC ACTGGGCACC AGCGCCAAGC CCAGCATCAC CTCCGGCCCA ATGGCCAGGC 1621 CCCCCGTCGT CGTCGAACCC GTCGCACCCA GCGACCCCAG CATCGTCAGC CTGATCGAGG 1681 AGAGCGCCAT CATCAATGCC GGAGCCCCTG AGATCGTCCC ACCCGCCCAC GGCGGCTTCA 1741 CCATCACCAG TAGCGAGACC ACCACACCCG CCATCCTCGA CGTCAGCGTC ACCTCCCACA 1801 CCACCACCTC CATCTTCCGC AACCCCGTGT TCACCGAGCC CAGCGTCACC CAGCCACAGC 1861 CCCCGGTCGA AGCCAACGGC CACATCCTGA TCAGCGCCCC AACCATCACC AGCCATCCCA 1921 TCGAAGAGAT CCCCCTGGAC ACCTTCGTCA TCAGTAGCTC CGACTCAGGC CCCACCAGTT 1981 CCACACCCGT CCCCGGCACC GCCCCACGCC CCAGGGTCGG GCTGTACTCC CGCGCCCTCC 2041 ATCAGGTCCA GGTCACCGAT CCCGCCTTCT TGAGTACCCC CCAAAGGCTG ATCACCTACG 2101 ACAATCCCGT GTACGAGGGC GAAGACGTCT CCGTGCAGTT CTCCCACGAC AGCATCCACA 2161 ACGCCCCCGA CGAAGCCTTC ATGGACATCA TCCGCCTCCA TCGCCCCGCC ATCGCCAGTA 2221 GGCGCGGGTT GGTCAGATAC TCCCGGATCG GCCAGAGAGG CAGCATGCAC ACCCGGTCAG 2281 GCAAACACAT CGGCGCAAGG ATCCACTACT TCTACGACAT CAGCCCCATC GCCCAGGCCG 2341 CCGAGGAGAT CGAGATGCAC CCCTTGGTCG CCGCCCAAGA CGACACCTTC GACATCTACG 2401 CCGAGAGCTT CGAGCCCGAC ATCAATCCCA CACAGCATCC CGTCACCAAC ATCAGCGACA 2461 CCTACCTGAC CAGTACCCCC AACACCGTCA CCCAGCCCTG GGGCAATACA ACCGTCCCGC 2521 TCTCCATCCC CAACGATCTG TTCCTGCAAA GCGGGCCCGA CATCACCTTC CCGACCGCCC 2581 CAATGGGCAC CCCATTCAGC CCCGTGACCC CCGCCCTGCC CACCGGGCCC GTCTTCATCA 2641 CCGGCAGCGG CTTCTACCTC CACCCCGCTT GGTACTTCGC CCGCAAGAGG CGCAAGAGGA 2701 TCCCACTGTT CTTCTCGGAC GTCGCCGCAT GAGATCAAGC TTATCGATAA TCAACCTCTG 2761 GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA 2821 TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT 2881 TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC 2941 AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT 3001 GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG 3061 GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC 3121 AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC 3181 ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC 3241 CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT 3301 CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCATC GATACCGTCG GCCGCTTCCC 3361 TTTAGTGAGG GTTAATGCTT CGAGCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA 3421 CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT 3481 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA 3541 TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT 3601 GTGGTATGGA ATTCTTAATT AACTAGCCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT 3661 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 3721 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 3781 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 3841 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 3901 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 3961 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 4021 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 4081 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 4141 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 4201 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 4261 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 4321 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTAATT AAGCTGTACA CTGTGGAATG 4381 TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 4441 TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 4501 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA 4561 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGACTGA CTAATTTTTT 4621 TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG 4681 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG 4741 GATCTGATCA GCACGTGTTG ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA 4801 CGACTCACTA TAGGAGGGCC ACCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG 4861 CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG 4921 ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC 4981 TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA 5041 CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC 5101 TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG 5161 TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT 5221 TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG 5281 TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA 5341 GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACACATGGC GATGCCTGCT 5401 TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG 5461 GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG 5521 GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC 5581 GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT 5641 GACCGACCAA GCGAATTCGC TAGAGGGAGG CTAACTGAAA CACGGAAGGA GACAATACCG 5701 GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG TGTTGGGTCG 5761 TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA 5821 CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG 5881 GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AG //