LOCUS P16L2W.TXT 6073 BP DS-DNA CIRCULAR SYN 26-MAR-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Plasmid from Blueheron biotechnology. Used for cloning codon optimized genes into it. FEATURES Location/Qualifiers promoter 113..310 /note="EF-1alpha promoter core" promoter 113..1291 /note="EF1a promoter and UTR" exon 311..343 /note="EF-1a exon 1" intron 344..1283 /note="EF-1a intron A" exon 1283..1291 /note="EF-1a exon 2 leader" CDS 1396..>2817 /note="Müller's L2" mRNA 2890..>3478 /note="WPRE" polyA_signal 3526..>3751 /note="SV40 late polyA" rep_origin 3752..4506 /note="MB1 ori" repeat_region 4681..4744 /note="SV40 21 bp repeats" promoter 4681..4855 /note="SV40 early promoter" rep_origin 4704..4855 /note="SV40 ori" mRNA 4753..4802 /note="SV40 transcription start points" promoter 4890..4945 /note="EM7" CDS 4965..5759 /note="Neomycin/G418 marker" polyA_signal 5807..6073 /note="HSV TK pA signal" polyA_site 5865..5883 /note="HSV TK pA site" BASE COUNT 1263 A 1842 C 1661 G 1307 T 0 OTHER ORIGIN - 1 CCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTGCAAAGA TGGATAAAGT TTTAAACAGA 61 GAGGAATCTT TGCAGCTAAT GGACCTTGTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 121 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 181 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 241 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 301 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 361 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 421 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 481 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 541 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 601 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 661 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 721 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 781 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 841 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 901 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 961 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1021 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1081 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1141 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1201 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1261 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA 1321 GATCCATCCT GTACGGAAGT GTTACTTCTG CTCTAAAAGC TGCGGAATTG TACCCGCGGC 1381 CGCTCTAGAG CCACCATGAG GCACAAGAGG AGCGCCAAGA GGACCAAGAG GGCCAGCGCC 1441 ACCCAGCTGT ACAAGACCTG CAAGCAGGCC GGCACCTGCC CCCCCGACAT CATCCCCAAG 1501 GTGGAGGGCA AGACCATCGC CGACCAGATC CTGCAGTACG GCAGCATGGG CGTGTTCTTC 1561 GGCGGCCTGG GCATCGGCAC CGGCAGCGGC ACCGGCGGCA GGACCGGCTA CATCCCCCTG 1621 GGCACCAGGC CCCCCACCGC CACCGACACC CTGGCCCCCG TGAGGCCCCC CCTGACCGTG 1681 GACCCCGTGG GCCCCAGCGA CCCCAGCATC GTGAGCCTGG TGGAGGAGAC CAGCTTCATC 1741 GACGCCGGCG CCCCCACCAG CGTGCCCAGC ATCCCCCCCG ACGTGAGCGG CTTCAGCATC 1801 ACCACCAGCA CCGACACCAC CCCCGCCATC CTGGACATCA ACAACACCGT GACCACCGTG 1861 ACCACCCACA ACAACCCCAC CTTCACCGAC CCCAGCGTGC TGCAGCCCCC CACCCCCGCC 1921 GAGACCGGCG GCCACTTCAC CCTGAGCAGC AGCACCATCA GCACCCACAA CTACGAGGAG 1981 ATCCCCATGG ACACCTTCAT CGTGAGCACC AACCCCAACA CCGTGACCAG CAGCACCCCC 2041 ATCCCCGGCA GCAGGCCCGT GGCCAGGCTG GGCCTGTACA GCAGGACCAC CCAGCAGGTG 2101 AAGGTGGTGG ACCCCGCCTT CGTGACCACC CCCACCAAGC TGATCACCTA CGACAACCCC 2161 GCCTACGAGG GCATCGACGT GGACAACACC CTGTACTTCA GCAGCAACGA CAACAGCATC 2221 AACATCGCCC CCGACCCCGA CTTCCTGGAC ATCGTGGCCC TGCACAGGCC CGCCCTGACC 2281 AGCAGGAGGA CCGGCATCAG GTACAGCAGG ATCGGCAACA AGCAGACCCT GAGGACCAGG 2341 AGCGGCAAGA GCATCGGCGC CAAGGTGCAC TACTACTACG ACCTGAGCAC CATCGACCCC 2401 GCCGAGGAGA TCGAGCTGCA GACCATCACC CCCAGCACCT ACACCACCAC CAGCCACGCC 2461 GCCAGCCCCA CCAGCATCAA CAACGGCCTG TACGACATCT ACGCCGACGA CTTCATCACC 2521 GACACCAGCA CCACCCCCGT GCCCAGCGTG CCCAGCACCA GCCTGAGCGG CTACATCCCC 2581 GCCAACACCA CCATCCCCTT CGGTGGCGCC TACAACATCC CCCTGGTGAG CGGCCCCGAC 2641 ATCCCCATCA ACATCACCGA CCAGGCCCCC AGCCTGATCC CCATCGTGCC CGGCAGCCCC 2701 CAGTACACCA TCATCGCCGA CGCCGGCGAC TTCTACCTGC ACCCCAGCTA CTACATGCTG 2761 AGGAAGAGGA GGAAGAGGCT GCCCTACTTC TTCAGCGACG TGAGCCTGGC CGCCTGAAAG 2821 CTTTTTGAAT TCTTTGGATC CACTAGTGGA TCCCCCGGGC TGCAGGAATT CGATATCAAG 2881 CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC 2941 TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT 3001 GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT 3061 GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA 3121 ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC 3181 CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG 3241 GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT 3301 TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT 3361 TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT 3421 CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT 3481 CGATACCGTC GGCCGCTTCC CTTTAGTGAG GGTTAATGCT TCGAGCAGAC ATGATAAGAT 3541 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG 3601 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 3661 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA 3721 GCAAGTAAAA CCTCTACAAA TGTGGTATGG AATTCTTAAT TAACTAGCCA TGACCAAAAT 3781 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 3841 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 3901 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 3961 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 4021 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 4081 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 4141 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 4201 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 4261 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 4321 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 4381 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 4441 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTAAT 4501 TAAGCTGTAC ACTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG 4561 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC 4621 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG 4681 TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC 4741 CCCATGACTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC 4801 TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG 4861 GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT CATCGGCATA 4921 GTATATCGGC ATAGTATAAT ACGACTCACT ATAGGAGGGC CACCATGATT GAACAAGATG 4981 GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC 5041 AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG 5101 TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC GAGGCAGCGC 5161 GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG 5221 AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC 5281 ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC 5341 TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA 5401 CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG 5461 CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAG GATCTCGTCG 5521 TGACACATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT 5581 TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC 5641 GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA 5701 TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG 5761 CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGAATTCG CTAGAGGGAG GCTAACTGAA 5821 ACACGGAAGG AGACAATACC GGAAGGAACC CGCGCTATGA CGGCAATAAA AAGACAGAAT 5881 AAAACGCACG GTGTTGGGTC GTTTGTTCAT AAACGCGGGG TTCGGTCCCA GGGCTGGCAC 5941 TCTGTCGATA CCCCACCGAG ACCCCATTGG GGCCAATACG CCCGCGTTTC TTCCTTTTCC 6001 CCACCCCACC CCCCAAGTTC GGGTGAAGGC CCAGGGCTCG CAGCCAACGT CGGGGCGGCA 6061 GGCCCTGCCA TAG //