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Dr. Adriana Zingone, MD, PhD

Mailing address: home 13202 Twinbrook Pkwy, Apt 203
Rockville, MD 20851
(240) 888-8927

work NIH-NCI National Naval Medical Center
Bldg 8, Room 5101
Bethesda, MD 20889
(301) 435-5424

Status: permanent resident

1987 Doctorial Degree in Medicine with the highest mark.
Faculty of Medicine University of Reggio Calabria at Catanzaro.

1988 Resident in Internal Medicine,Faculty of Medicine,
University of Reggio Calabria at Catanzaro.

1990-1991 Fellowship for clinical research, awarded by the Department of Education, Calabria Region.

1992 Specialist in Internal Medicine.

1992 Fellowship awarded by the National Research Council (CNR)

1993-1994 Clinical staff fellow at the Department Clinical and Experimental Medicine, University of Reggio Calabria at Catanzaro. During these years Dr. Zingone developed a deep interest for molecular diagnosis and physiopathologic aspects of metabolic and genetic diseases, including diabetes, dyslypidemias and cystic fibrosis.

1993-1995 Research fellow at the Department of Clinical and Experimental Medicine "G. Salvatore", University of Catanzaro (Grant issued by Region Calabria)

1996-2000 PhD in Oncology. Length of the Course : 4 years

2000 PhD degree awarded in Oncology

1997-2000 Visiting Fellow in Dr Janice Y. Chou's laboratory,
NICHD (National Institute of Child Health and Human Development) at the National Institute of Health, in Bethesda, MD, USA.

In this laboratory Dr. Zingone successfully completed a project concerning the possibility to treat a murine model of Glycogen Storage Disease Type 1a by means of recombinant adenovirus vector carrying the coding sequence of murine glucose-6-phosphatase. She also generated a lentiviral vector carrying the glucose-6-phosphatase cDNA and also a lentivirus expressing a reporter gene (EGFP) as control vector, in collaboration with Dr. S. Arya (NCI at NIH, Bethesda)

2000-2001 Scientist in the Laboratory of Molecular Biology,
Institute "G.Gaslini", Genova, Italy Luigi Varesio, Director

In this laboratory Dr. Zingone utilized a lentiviral vector for the in vivo gene therapy of newborn mice deficient in the glucose-6-phosphatase enzyme.

2002-2003 Visiting Fellow in Dr. Michael Kuehl's laboratory
Genetics Branch, Center for Cancer Research, National Cancer Institute, NIH

2003-present Research Fellow in Dr. Michael Kuehl's laboratory
Genetics Branch, Center for Cancer Research, National Cancer Institute, NIH

In the laboratory of Dr. Michael Kuehl, Dr. Zingone is involved in two different research programs: the identification and characterization of the oncogenic events involved in the molecular pathogenesis of human multiple myeloma (MM), and the generation of 'in vitro" and "in vivo" models of multiple myeloma to better understand the consequences of the oncogenic events occuring in the tumors.

Dr. Zingone has shown that normal FGFR3 cooperates with c-MYC to increase tumorogenesis in F1 mice that express both transgenes. Dr. Zingone has generated cultures of polyclonal plasmablasts from normal human peripheral blood cells, and has demonstrated that she can successfully infect them with retroviral vectors. She is just beginning experiments to infect normal plasmablasts with retroviral vectors so that it will be possible to assess the effects of combinations of SV40 T antigen, activated K-RAS, HTERT, and a variety of oncogenes that are altered or activated in multiple myeloma. Dr. Zingone has prepared lentiviral vectors so that combinations of oncogenes and viral products that inactivate tumor suppressor genes can be introduced into non-proliferative pre-malignant MGUS and malignant MM tumor cells.

Abstracts

1. Perrotti, N., Lucia, I., Zingone, A., Palumbo, G., Falcioni, R., and Sacchi, A. Alpha 6-beta integrin complex: a novel endogenous substrate for the insulin receptor associated tyrosyne kinase. IVth International Symposium on Insulin Receptor and Insulin Action. Verona, Italy, Sept, 1990 Abstract p60-61.

2. Marasco, O., Melina, F., Mele, E., Quaresima, B., Zingone, A., Focarelli, E., Picciotti, E., Martelli, M.L., Fotino, L., Vigna, M.F., Baudi, F., Dominijanni, A., Angotti, E., Pujia, A., and Perrotti, N.
Frequency and distribution of an allele for the apolipoprotein AI-CIII in a Calabrian population.
Convegno Congiunto SIBBM-AGI, Porto Conte (SS), Italy, Oct, 1991.

3. Quaresima, B., Marasco, O., Melina, F., Mele, E., Zingone, A., Focarelli, E., Picciotti, E., Martelli, M.L., Fotino, L., Vigna, M.F., Baudi, F., Dominijanni, A., Angotti, E., Perrotti, N., Porcellini, A., Colonna, A., Mattioli, P.L., Avvedimento V.E., and Costanzo, F. Analysis of three polymorphic markers (RFLPs) in the apolipoprotein AI-CIII gene cluster. Riunione Interregionale Societá Italiana di Biochimica, Palermo, Italy, 1992.

4. Perrotti, N., De Amicis, F., Zingone, A., Dominijanni, A., Veneziani B.M., and Tramontano, D.
Expression of genes correlates with the cell cycle in cultured thyroid cells. In Decime Giornate Italiane della tiroide, Palermo, Italy, Nov, 1992.

5. Guadagnino, V., Caroleo, V., Zingone, A., Loiacono, L., Perrotti, N., and Tramontano, D.
Identification of HCV genotypes in Calabria Region; A preliminary report. Fourth International Conference on Current Trends in Chronically Evolving Viral Hepatitis, Milan, Italy, April, 1995.

6. Dominijanni, A., Zingone, A., Villone, G., De Amicis, F., Perrotti, N., and Tramontano, D.
The expression of cdc-25 in rat thyroid cells. II Italian Meeting of Molecular Oncology. Positano, Italy, May, 1995.

7. Dominijanni, A., Zingone, A., Villone, G., De Amicis, F., Perrotti, N., and Tramontano, D.
The expression of cdc-25 in rat thyroid cells. Atti Convegno Nazionale Congiunto: Associazione di Biologia Cellulare e del Differenziamento, Associazione Genetica Italiana, Societá di Biofisica e Biologia Molecolare, Societá Italiana di Microbiologia Generale e Biotecnologie Microbiche, Monte Silvano, Italy, Oct, 1995.

8. Perticone, F., Pugliese, F., Ceravolo, R., Cosco, C., Trapasso, M.P., Zingone, A., Perrotti, N., and Mattioli, P.L. Association of angiotensin Converting Enzyme Gene with Blood pressure Variabilty and Ventricular Mass in an Italian population. The American Society of Hypertension XIth Scientific Meeting, Boston, June, 1996.

9. Zingone, A., Malatesta, P., Dominijanni, A., Imperatore, G., Iovino, E., Vaccaro, O., Riccardi, G., Tramontano, D., and Perrotti N. Polimorfismo ACE e tolleranza glucidica. XVI National Meeting Italian Society of Diabetes (SID), Napoli, Italy, May, 1996.

10. Zingone, A., Cultraro, C. M., Bean, C., Janz, S., and Kuehl M.
Ectopic lymphoid expression of wild-type FGFR3 appears to be oncogenic in transgenic mice.
NCI Third Annual Fellow and Young Investigators Retreat, Ocean City, MD, Feb, 2003.

11. Dib, A., Peterson, T.R., Raduca-Grace, L., Zingone, A., Zhan, F., Hanamura, I., Barlogie, B., Shaughenessy Jr, J., and Kuehl, W.M. Duality of p18INK4c expression in proliferative multiple myeloma: bi-allelic deletion vs increased expression. NCI Fellow and Young Investigators Retreat. Williamsburg, VA, Feb, 2005.

12. Arya, S.K., Sashdeva, G., Zingone, A., Kachapati, K., and Horikawa, I. Two hit strategy for arresting tumor cell growth. International Symposium on Translational Research: Apoptosis & Cancer. Trivandrum, India, Dec, 2005.

13. Holczbauer, A., Zingone, A., Kachapati, K., and Suresh K. Arya. Toward Gene Therapy of Cancer: A Model for Selective Expression of Anti-life and Pro-death Transgenes. American Society of Gene Therapy, Boston, June, 2008.

Publications:

1. Zingone, A., Lucia, I., Perrotti, N. Le basi molecolari dell'insulino-resistenza.
Fisiopatologia endocrino-metabolica e diagnostica di laboratorio. Giaccetto, C., Macchia, V. Ed. Bios, Unilibro, 1990.

2. Marasco, O., Melina, F., Mele, E., Quaresima, B., Zingone, A., Focarelli, E., Picciotti, E., Martelli, M.L., Fotino,L.,Vigna, M.F., Baudi, F., Dominijanni, A., Angotti, E., Pujia, A., Perrotti, N., Colonna, A., Mattioli, P.L., Porcellini, A., Costanzo, F., and Avvedimento, V.E. Linkage disequilibrium of three polymorphic RFLPS markers in the apolipoprotein AI-CIII gene cluster on chromosome 11. Human Genetics 91:169-174 (1993).

3. Quaresima, B., Marasco, O., Melina, F., Mele, E., Zingone, A., Focarelli, E., Picciotti, E., Martelli, M.L., Fotino, L., Vigna,M.F., Baudi, F., Dominijanni, A., Angotti, E., Perrotti, N., Porcellini, A., Colonna, A., Mattioli, P.L., Avvedimento V.E., and Costanzo F. Analysis of three polymorphic markers (RFLPs) in the apolipoprotein AI-CIII gene cluster. The Italian Journal of Biochemistry, 42:134-135 (1993).

4. Perrotti, N., De Amicis, F., Zingone, A., Dominijanni, A., Veneziani B.M. and Tramontano, D.
Expression of genes related to cell cycle in thyroid cells in culture. Acta Medica Romana 30:12 (1992).

5. Zingone, A., Dominijanni, A., Mele, E., Marasco, O., Melina, F., Minchella, P., Quaresima, B., Tiano, M.T., Gnasso, A., Pujia A., and Perrotti, N. Deletion plymorphism in the gene for angiotensin converting enzyme is associated to elevated fasting blood glucose levels. Human Genetics 94: 207-209 (1994).

6. Pujia, A., Gnasso, A., Irace, C., Dominijanni, A., Zingone, A., Perrotti, N., Colonna, A., and P.L.Mattioli. Association between ACE-D/D polymorphism and hypertension in type II diabetic subjects. Journal of Human Hypertension 8: 687-691 (1994).

7. Zingone, A., Dominijanni, A., Pujia, A., Irace, C., Tramontano, D., Colonna, A., Perrotti N.
Deletion polymorphism in the gene for angiotensin converting enzyme is associated to insulin resistance-hypertension syndrome. The Italian Journal of Biochemistry 4:179-180 (1994).

8. Dominijanni, A., Zingone, A.,Villone, G., De Amicis, F., Perrotti, N., and Tramontano D. The expression of cdc 25 in rat thyroid cells. Journal of Endocrinological Investigation 18(S5):147(1995).

9. Guadagnino, V., Caroleo, B., Zingone, A., Loiacono, L., Perrotti, N., and Tramontano, D. Identification of HCV genotypes in Calabria Region. Pathologie Biologie 44:464-467 (1996).

10. Perticone, F., Ceravolo, R., Cosco, C., Trapasso, M.P., Zingone, A., Malatesta P., Perrotti, N., Mattioli, P.L. Deletion Polimorphism of angiotension-converting-enzyme gene and left ventricular hypertrophy in a south italian population. The Journal of American College of Cardiologo 29:365-369 (1997).

11. Perticone, F., Ceravolo, R., Cosco, C., Maio, R., Ventura, G., Zingone, A., Perrotti, N., Mattioli, P.L. Angiotensin-Converting Enzyme gene polymorphism is associated with endothelium-dependent vasodilation in never treated hypertensive patients. Hypertension 31:900-905 (1998).

12. Zingone, A., Hiraiwa, H., Pan, C.J., Lin, B., Chen, H., Ward J.M. and Chou, J.Y. Correction of Glycogen Storage Disease type 1a in a mouse model by gene therapy. J Biol Chem 275:828-832 (2000).

13. Zingone, A., Seidel, J., Aloi, L., Jadoga, E.M., Chou, JY., Green, M.V., Eckelman W.C. Monitoring the Correction of Glycogen Storage Disease Type 1a in a Mouse Model using 18F-FDG and a Dedicated Animal Scanner. Life Sciences 71:1293-1301 (2002).

14. Chou, J.Y., Zingone, A., Pan, C.J. Adenovirus-mediated gene therapy in a mouse model of Glycogen Storage Disease type 1a. Eur J Pediatr 161:S56-S61 (2002).

15. Salani, B., Damonte, P., Zingone, A., Barbieri, O., Chou, J.Y., D'Costa, J., Arya, S.K., Eva, A., and L Varesio. Newborn liver gene transfer by an HIV-2- based lentiviral vector. Gene Therapy 12:803-814 (2005).

16. Dib, A., Peterhson, T.R., Raduca-Grace, L., Zingone, A., Zhan, F., Hanamura, I., Barlogie, B., Shaughenessy, Jr, J., and Kuehl, W.M. Paradoxical expression of INK4c in proliferative multiple myeloma tumors: bi-allelic deletion vs increased expression. Cell Div 18;1-23 (2006).
17. Zingone, A., Cultraro C. M.,Bean C.,Morse H., Janz S.,and Kuehl, W.M.
Ectopic lymphoid expression of wild-type FGFR3 cooperates with c-MYC to increase tumorogenesis in transgenic mice. In preparation.

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