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Materials required for completion of all steps:

  • Computer (ideally 16GB+ RAM, 5GB+ Hard-drive space, Quad-core processor, or above)
  • Scatter reference beads (spherical beads, known diameter & refractive index)
  • Fluorescence reference beads (MESF/ERF beads)
  • Flow Cytometer and Analysis software
  • FCMPASS Software

Overview of steps:

  1. Software Installation
  2. Software Usage
  3. Bead acquisition
  4. Light scatter calibration
  5. Fluorescence calibration
  6. Writing data to .fcs files
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