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Center For Cancer Research National Cancer Institute
EIB Microscopy and Digital Imaging Facility

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The SPOT-2 is a cooled, color CCD (charge-coupled device) camera with 4 different imaging modes:

24-bit RGB color images for regular color images (i.e., brightfield histology, bright fluorescence)

8-bit monochrome images for increased sensitivity when RGB filters are removed, low intensity fluorescence

36-bit RGB color images for increased dynamic in color contrast

12-bit monochrome images for increased dynamic range coupled with high sensitivity, quantitative fluorescence imaging