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Center For Cancer Research National Cancer Institute
Confocal Microscopy Core Facility

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The Confocal Microscopy Core Facility provides "open access" confocal laser scanning microscopy (LSM) services to all CCR investigators. The Facility's equipment includes a:

  • Zeiss LSM 510 META NLO for 2-photon imaging system
  • Zeiss LSM 510 UV for imaging UV and VIS fluorophores
  • Zeiss LSM 710 NLO for 2-photon imaging system
  • Zeiss LSM 780 for higher sensitivity imaging

Confocal software  simultaneous or sequential 3-channel (24-bit) acquisition of two-dimensional (2D), 3D, and 4D data. Five additional processing workstations are available for post-acquisition analysis, and producing publication-quality images.

Imaging of both fixed specimens and live cells is provided. For live cell imaging, cells are placed in a temperature-controlled, perfusable enclosed chamber or an open chamber allowing acquisition over several minutes to several hours. Fluorescent recovery after photobleaching (FRAP), fluorescence resonance energy transfer (FRET), and co-localization studies can be conducted as well as imaging of UV excitable dyes. The infrared laser of the 2-photon system facilitates imaging of thick specimens and minimizes tissue damage while the META detector provides multi-spectral imaging. Several software packages are available to users including:

  • Zeiss LSM 4.2
  • Zeiss ZEN
  • Bitplane Imaris for volume/surface rendering, tracking, and measurements
  • Media Cybernetics Image Pro Plus/Premier and 3D constructor
  • NIH ImageJ
  • Adobe Photoshop