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Antibody Validation Table

Target Protein

Company

Recommended Dilution

Source

Weight

Cross Reactivity

Positive Control

Cross Reactivity Validated by Western Blotting (Canine Samples)

Akt

COP:Cell Signaling

1:1000

Rabbit

60kDa

H,M,R

K7M2

Protocol for Normal Tissues Western Blot
AKT Normal Tissues.jpg
Protocol For Tumor Tissues Western Blot
Western Blot-Tumor tissue

pAKT

COP:Cell Signaling

1:1000

Rabbit

60kDa

H,M,R,C,Hm

K7M2
NIH 3T3

Protocol for Normal Tissue Western Blot
pAKT Normal Tissues Blot
Protocol for Tumor Tissue Western Blot
Western Blot-Tumor tissue

elF4E

COP:Cell Signaling

1:1000

Rabbit

25kDa

H,M,R,Z

K7M2

Protocol for Normal Tissue Western Blot 
eIF4E Normal Tissue Blot
Protocol for Tumor Tissue Western Blot
eIF4E Tumor Tissue Blot

P-elF4E 

COP:Cell Signaling

1:1000

Rabbit

25 kDa

H,M,R,Z

K7M2

Protocol for Normal Tissue Western Blot
peIF4E Normal Tissue Blot
Protocol for Tumor Tissue Western Blot
peIF4E Tumor Tissue Blot

Ezrin

COP:Sigma

1:1000

Mouse

80 kDa

H

K7M2

Protocol for Normal Tissue Western Blot
Ezrin Normal Tissue Blot
Protocol for Tumor Tissue Western Blot
Ezrin Tumor Tissue Blot

p-Ezrin

COP:Cell Signaling

1:1000

Rabbit

80 kDa

 

K7M2
NIH 3T3

Protocol for Normal Tissue Western Blot
pEzrin Normal Tissue Blot
Protocol for Tumor Tissue Western Blot
pEzrin Tissue Blot

ERM

COP:Cell Signaling

1:1000

Rabbit

75,80 kDa

H,M,R,MK

K7M2
A431

Protocol for Normal Tissue Western Blot
ERM Normal Tissue Blot
Protocol for Tumor Tissue Western Blot
ERM Tumor Tissue Blot

P-ERM

COP:Cell Signaling

1:1000

Rabbit

75, 80 kDa

H,M,R,Mk

K7M2

Protocol for Normal Tissue Western Blot
pERM Normal Tissue Blot
Protocol for Tumor Tissue Western Blot
pERM Tumor Tissue Blot

P-4E-BP1

COP:Cell Signaling

1:1000

Rabbit

15-20 kDa

H,M,R,Z

K7M2

COP:Protocol
COP:Western Blot-Normal tissue
COP:Protocol-Tumor
COP:Western Blot-Tumor tissue

p44/42 Map Kinase

COP:Cell Signaling

1:1000

Rabbit

42,44 kDa

H,M,R,Hm,Z

K7M2

COP:Protocol
COP:Western Blot-Normal tissue
 COP:Protocol-Tumor
COP:Western Blot-Tumor tissue

P-P44/42 Map Kinase

COP: Cell Signaling

1:2000

Mouse

42,44 kDa

H,M,R,Hm,Z

K7M2

COP:Protocol
COP:Western Blot-Normal tissue
COP:Protocol-Tumor
COP:Western Blot-Tumor tissue

p70 S6 Kinase

COP:Cell Signaling

1:1000

Mouse

70,85 kDa

H,M,R,Mk

K7M2
NIH 3T3

COP:Protocol
COP:Western Blot-Normal tissue
COP:Protocol-Tumor
COP:Western Blot-Tumor tissue

P-p70 S6 Kinase

COP:Cell Signaling

1:1000

Mouse

70,85 kDa

H,M,R,Mk

K7M2
NIH 3T3

COP:Protocol  
COP:Western Blot-Normal tissue
COP:Protocol-Tumor
COP:Western Blot-Tumor tissue

For any questions please contact Christina Mazcko at mazckoc@mail.nih.gov

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