CCR COP Antibody Validation Project : Antibody Validation Table

Antibody Validation Table

Target Protein	Company	Recommended Dilution	Source	Weight	Cross Reactivity	Positive Control	Cross Reactivity Validated by Western Blotting (Canine Samples)
Akt	COP:Cell Signaling	1:1000	Rabbit	60kDa	H,M,R	K7M2	Protocol for Normal Tissues Western Blot AKT Normal Tissues.jpg Protocol For Tumor Tissues Western Blot Western Blot-Tumor tissue
pAKT	COP:Cell Signaling	1:1000	Rabbit	60kDa	H,M,R,C,Hm	K7M2 NIH 3T3	Protocol for Normal Tissue Western Blot pAKT Normal Tissues Blot Protocol for Tumor Tissue Western Blot Western Blot-Tumor tissue
elF4E	COP:Cell Signaling	1:1000	Rabbit	25kDa	H,M,R,Z	K7M2	Protocol for Normal Tissue Western Blot eIF4E Normal Tissue Blot Protocol for Tumor Tissue Western Blot eIF4E Tumor Tissue Blot
P-elF4E	COP:Cell Signaling	1:1000	Rabbit	25 kDa	H,M,R,Z	K7M2	Protocol for Normal Tissue Western Blot peIF4E Normal Tissue Blot Protocol for Tumor Tissue Western Blot peIF4E Tumor Tissue Blot
Ezrin	COP:Sigma	1:1000	Mouse	80 kDa	H	K7M2	Protocol for Normal Tissue Western Blot Ezrin Normal Tissue Blot Protocol for Tumor Tissue Western Blot Ezrin Tumor Tissue Blot
p-Ezrin	COP:Cell Signaling	1:1000	Rabbit	80 kDa		K7M2 NIH 3T3	Protocol for Normal Tissue Western Blot pEzrin Normal Tissue Blot Protocol for Tumor Tissue Western Blot pEzrin Tissue Blot
ERM	COP:Cell Signaling	1:1000	Rabbit	75,80 kDa	H,M,R,MK	K7M2 A431	Protocol for Normal Tissue Western Blot ERM Normal Tissue Blot Protocol for Tumor Tissue Western Blot ERM Tumor Tissue Blot
P-ERM	COP:Cell Signaling	1:1000	Rabbit	75, 80 kDa	H,M,R,Mk	K7M2	Protocol for Normal Tissue Western Blot pERM Normal Tissue Blot Protocol for Tumor Tissue Western Blot pERM Tumor Tissue Blot
P-4E-BP1	COP:Cell Signaling	1:1000	Rabbit	15-20 kDa	H,M,R,Z	K7M2	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue
p44/42 Map Kinase	COP:Cell Signaling	1:1000	Rabbit	42,44 kDa	H,M,R,Hm,Z	K7M2	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue
P-P44/42 Map Kinase	COP: Cell Signaling	1:2000	Mouse	42,44 kDa	H,M,R,Hm,Z	K7M2	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue
p70 S6 Kinase	COP:Cell Signaling	1:1000	Mouse	70,85 kDa	H,M,R,Mk	K7M2 NIH 3T3	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue
P-p70 S6 Kinase	COP:Cell Signaling	1:1000	Mouse	70,85 kDa	H,M,R,Mk	K7M2 NIH 3T3	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue

For any questions please contact Christina Mazcko at mazckoc@mail.nih.gov

CCR COP Antibody Validation Project : Antibody Validation Table

Antibody Validation Table

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